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Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN

Transposable elements are present in a wide variety of organisms; however, our understanding of the diversity of mechanisms involved in their activation is incomplete. In this study, we analyzed the transcriptional activation of the ONSEN retrotransposon, which is activated by high-temperature stres...

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Autores principales: Nozawa, Kosuke, Masuda, Seiji, Saze, Hidetoshi, Ikeda, Yoko, Suzuki, Takamasa, Takagi, Hiroki, Tanaka, Keisuke, Ohama, Naohiko, Niu, Xiaoying, Kato, Atsushi, Ito, Hidetaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340270/
https://www.ncbi.nlm.nih.gov/pubmed/35923888
http://dx.doi.org/10.3389/fpls.2022.899105
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author Nozawa, Kosuke
Masuda, Seiji
Saze, Hidetoshi
Ikeda, Yoko
Suzuki, Takamasa
Takagi, Hiroki
Tanaka, Keisuke
Ohama, Naohiko
Niu, Xiaoying
Kato, Atsushi
Ito, Hidetaka
author_facet Nozawa, Kosuke
Masuda, Seiji
Saze, Hidetoshi
Ikeda, Yoko
Suzuki, Takamasa
Takagi, Hiroki
Tanaka, Keisuke
Ohama, Naohiko
Niu, Xiaoying
Kato, Atsushi
Ito, Hidetaka
author_sort Nozawa, Kosuke
collection PubMed
description Transposable elements are present in a wide variety of organisms; however, our understanding of the diversity of mechanisms involved in their activation is incomplete. In this study, we analyzed the transcriptional activation of the ONSEN retrotransposon, which is activated by high-temperature stress in Arabidopsis thaliana. We found that its transcription is significantly higher in the Japanese ecotype Kyoto. Considering that transposons are epigenetically regulated, DNA methylation levels were analyzed, revealing that CHH methylation was reduced in Kyoto compared to the standard ecotype, Col-0. A mutation was also detected in the Kyoto CMT2 gene, encoding a CHH methyltransferase, suggesting that it may be responsible for increased expression of ONSEN. CHH methylation is controlled by histone modifications through a self-reinforcing loop between DNA methyltransferase and histone methyltransferase. Analysis of these modifications revealed that the level of H3K9me2, a repressive histone marker for gene expression, was lower in Kyoto than in Col-0. The level of another repressive histone marker, H3K27me1, was decreased in Kyoto; however, it was not impacted in a Col-0 cmt2 mutant. Therefore, in addition to the CMT2 mutation, other factors may reduce repressive histone modifications in Kyoto.
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spelling pubmed-93402702022-08-02 Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN Nozawa, Kosuke Masuda, Seiji Saze, Hidetoshi Ikeda, Yoko Suzuki, Takamasa Takagi, Hiroki Tanaka, Keisuke Ohama, Naohiko Niu, Xiaoying Kato, Atsushi Ito, Hidetaka Front Plant Sci Plant Science Transposable elements are present in a wide variety of organisms; however, our understanding of the diversity of mechanisms involved in their activation is incomplete. In this study, we analyzed the transcriptional activation of the ONSEN retrotransposon, which is activated by high-temperature stress in Arabidopsis thaliana. We found that its transcription is significantly higher in the Japanese ecotype Kyoto. Considering that transposons are epigenetically regulated, DNA methylation levels were analyzed, revealing that CHH methylation was reduced in Kyoto compared to the standard ecotype, Col-0. A mutation was also detected in the Kyoto CMT2 gene, encoding a CHH methyltransferase, suggesting that it may be responsible for increased expression of ONSEN. CHH methylation is controlled by histone modifications through a self-reinforcing loop between DNA methyltransferase and histone methyltransferase. Analysis of these modifications revealed that the level of H3K9me2, a repressive histone marker for gene expression, was lower in Kyoto than in Col-0. The level of another repressive histone marker, H3K27me1, was decreased in Kyoto; however, it was not impacted in a Col-0 cmt2 mutant. Therefore, in addition to the CMT2 mutation, other factors may reduce repressive histone modifications in Kyoto. Frontiers Media S.A. 2022-07-18 /pmc/articles/PMC9340270/ /pubmed/35923888 http://dx.doi.org/10.3389/fpls.2022.899105 Text en Copyright © 2022 Nozawa, Masuda, Saze, Ikeda, Suzuki, Takagi, Tanaka, Ohama, Niu, Kato and Ito. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Nozawa, Kosuke
Masuda, Seiji
Saze, Hidetoshi
Ikeda, Yoko
Suzuki, Takamasa
Takagi, Hiroki
Tanaka, Keisuke
Ohama, Naohiko
Niu, Xiaoying
Kato, Atsushi
Ito, Hidetaka
Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN
title Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN
title_full Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN
title_fullStr Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN
title_full_unstemmed Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN
title_short Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN
title_sort epigenetic regulation of ecotype-specific expression of the heat-activated transposon onsen
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340270/
https://www.ncbi.nlm.nih.gov/pubmed/35923888
http://dx.doi.org/10.3389/fpls.2022.899105
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