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Direct pulp capping with autologous bone marrow derived stem cells in dogs
Bone-marrow derived stem cells (BMSCs) can differentiate into several mesenchymal cell lines that are suitable for bone and dental tissue engineering. This study was aimed to assess the efficacy of cell therapy in direct pulp capping (DPC) of canine teeth using autologous BMSCs along with collagen/h...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Urmia University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340288/ https://www.ncbi.nlm.nih.gov/pubmed/35919843 http://dx.doi.org/10.30466/vrf.2020.122172.2874 |
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author | Farzad-Mohajeri, Saeed Pedram, Mir Sepehr Saeedifar, Nasrin Mashhadi-Abbas, Fatemeh Dehghan, Mohammad Mehdi Bahrami, Naghmeh Hashemikamangar, Sedighe Sadat |
author_facet | Farzad-Mohajeri, Saeed Pedram, Mir Sepehr Saeedifar, Nasrin Mashhadi-Abbas, Fatemeh Dehghan, Mohammad Mehdi Bahrami, Naghmeh Hashemikamangar, Sedighe Sadat |
author_sort | Farzad-Mohajeri, Saeed |
collection | PubMed |
description | Bone-marrow derived stem cells (BMSCs) can differentiate into several mesenchymal cell lines that are suitable for bone and dental tissue engineering. This study was aimed to assess the efficacy of cell therapy in direct pulp capping (DPC) of canine teeth using autologous BMSCs along with collagen/hydroxyapatite hybrid scaffold in terms of the quantity and quality of calcified bridge formation. The teeth were randomly divided into three groups of DPC with mineral trioxide aggregate (MTA), hydroxyapatite/collagen hybrid scaffold alone and BMSCs with hydroxyapatite/collagen hybrid scaffold. DPC was performed under general anesthesia in cavities prepared on the buccal surfaces of mandibular and maxillary premolars of the same dogs from which, stem cells had been isolated. All cavities were then restored with light-cure resin modified glass ionomer cement. Histomorphometric assessments after 12 weeks showed formation of dentinal bridge following DPC with BMSCs and MTA. The efficacy of MTA for calcified bridge formation following DPC was significantly higher than that of BMSCs plus hybrid scaffold. According to the present study, we concluded DPC using BMSCs and hybrid scaffold did not provide clinically noticeable results in canine patients. |
format | Online Article Text |
id | pubmed-9340288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Urmia University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-93402882022-08-01 Direct pulp capping with autologous bone marrow derived stem cells in dogs Farzad-Mohajeri, Saeed Pedram, Mir Sepehr Saeedifar, Nasrin Mashhadi-Abbas, Fatemeh Dehghan, Mohammad Mehdi Bahrami, Naghmeh Hashemikamangar, Sedighe Sadat Vet Res Forum Original Article Bone-marrow derived stem cells (BMSCs) can differentiate into several mesenchymal cell lines that are suitable for bone and dental tissue engineering. This study was aimed to assess the efficacy of cell therapy in direct pulp capping (DPC) of canine teeth using autologous BMSCs along with collagen/hydroxyapatite hybrid scaffold in terms of the quantity and quality of calcified bridge formation. The teeth were randomly divided into three groups of DPC with mineral trioxide aggregate (MTA), hydroxyapatite/collagen hybrid scaffold alone and BMSCs with hydroxyapatite/collagen hybrid scaffold. DPC was performed under general anesthesia in cavities prepared on the buccal surfaces of mandibular and maxillary premolars of the same dogs from which, stem cells had been isolated. All cavities were then restored with light-cure resin modified glass ionomer cement. Histomorphometric assessments after 12 weeks showed formation of dentinal bridge following DPC with BMSCs and MTA. The efficacy of MTA for calcified bridge formation following DPC was significantly higher than that of BMSCs plus hybrid scaffold. According to the present study, we concluded DPC using BMSCs and hybrid scaffold did not provide clinically noticeable results in canine patients. Urmia University Press 2022-06 2022-06-15 /pmc/articles/PMC9340288/ /pubmed/35919843 http://dx.doi.org/10.30466/vrf.2020.122172.2874 Text en © 2022 Urmia University. All rights reserved https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Farzad-Mohajeri, Saeed Pedram, Mir Sepehr Saeedifar, Nasrin Mashhadi-Abbas, Fatemeh Dehghan, Mohammad Mehdi Bahrami, Naghmeh Hashemikamangar, Sedighe Sadat Direct pulp capping with autologous bone marrow derived stem cells in dogs |
title | Direct pulp capping with autologous bone marrow derived stem cells in dogs |
title_full | Direct pulp capping with autologous bone marrow derived stem cells in dogs |
title_fullStr | Direct pulp capping with autologous bone marrow derived stem cells in dogs |
title_full_unstemmed | Direct pulp capping with autologous bone marrow derived stem cells in dogs |
title_short | Direct pulp capping with autologous bone marrow derived stem cells in dogs |
title_sort | direct pulp capping with autologous bone marrow derived stem cells in dogs |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340288/ https://www.ncbi.nlm.nih.gov/pubmed/35919843 http://dx.doi.org/10.30466/vrf.2020.122172.2874 |
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