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Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats

Skin flap necrosis has been remained as an unsolved problem in plastic and reconstructive surgeries. Here, we explored the effects of metformin post-treatment on random skin flap survival in rats. An 8.00 × 2.00 cm dorsal skin flap was created in 24 rats and they were then divided into three groups...

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Autores principales: Karimipour, Mojtaba, Farjah, Gholam hossein, Hassanzadeh, Morteza, Zirak Javanmard, Masoumeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340296/
https://www.ncbi.nlm.nih.gov/pubmed/35919859
http://dx.doi.org/10.30466/vrf.2020.124592.2917
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author Karimipour, Mojtaba
Farjah, Gholam hossein
Hassanzadeh, Morteza
Zirak Javanmard, Masoumeh
author_facet Karimipour, Mojtaba
Farjah, Gholam hossein
Hassanzadeh, Morteza
Zirak Javanmard, Masoumeh
author_sort Karimipour, Mojtaba
collection PubMed
description Skin flap necrosis has been remained as an unsolved problem in plastic and reconstructive surgeries. Here, we explored the effects of metformin post-treatment on random skin flap survival in rats. An 8.00 × 2.00 cm dorsal skin flap was created in 24 rats and they were then divided into three groups (n = 8) including Control, metformin (Met) 50.00 mg kg(-1) and Met 100 mg kg(-1). All animals were administrated orally until seven days after flap surgery. Flap survival, the number of blood vessels and mast cells in the flap tissues were analyzed. Vascular endothelial growth factor (VEGF) expression levels in flap tissues was also determined using immunohistochemical methods. The percentage of survival area in Met 50.00 mg kg(-1 )and Met 100 mg kg(-1 )groups were significantly higher compared to control. The blood vessel density and the VEGF positive cells in the viable areas of flaps showed a significant increase in Met 50.00 mg kg(-1) group compared to control group. The results of this study suggested that treatment with metformin, especially with low dose following skin flap surgery was effective in improving the flap survival and increasing the neovascularization in the flaps tissues of rats.
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spelling pubmed-93402962022-08-01 Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats Karimipour, Mojtaba Farjah, Gholam hossein Hassanzadeh, Morteza Zirak Javanmard, Masoumeh Vet Res Forum Original Article Skin flap necrosis has been remained as an unsolved problem in plastic and reconstructive surgeries. Here, we explored the effects of metformin post-treatment on random skin flap survival in rats. An 8.00 × 2.00 cm dorsal skin flap was created in 24 rats and they were then divided into three groups (n = 8) including Control, metformin (Met) 50.00 mg kg(-1) and Met 100 mg kg(-1). All animals were administrated orally until seven days after flap surgery. Flap survival, the number of blood vessels and mast cells in the flap tissues were analyzed. Vascular endothelial growth factor (VEGF) expression levels in flap tissues was also determined using immunohistochemical methods. The percentage of survival area in Met 50.00 mg kg(-1 )and Met 100 mg kg(-1 )groups were significantly higher compared to control. The blood vessel density and the VEGF positive cells in the viable areas of flaps showed a significant increase in Met 50.00 mg kg(-1) group compared to control group. The results of this study suggested that treatment with metformin, especially with low dose following skin flap surgery was effective in improving the flap survival and increasing the neovascularization in the flaps tissues of rats. Urmia University Press 2022-06 2022-06-15 /pmc/articles/PMC9340296/ /pubmed/35919859 http://dx.doi.org/10.30466/vrf.2020.124592.2917 Text en © 2022 Urmia University. All rights reserved https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Karimipour, Mojtaba
Farjah, Gholam hossein
Hassanzadeh, Morteza
Zirak Javanmard, Masoumeh
Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats
title Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats
title_full Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats
title_fullStr Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats
title_full_unstemmed Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats
title_short Post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats
title_sort post-treatment with metformin improves random skin flap survival through promoting angiogenesis in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340296/
https://www.ncbi.nlm.nih.gov/pubmed/35919859
http://dx.doi.org/10.30466/vrf.2020.124592.2917
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