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PILS proteins provide a homeostatic feedback on auxin signaling output

Multiple internal and external signals modulate the metabolism, intercellular transport and signaling of the phytohormone auxin. Considering this complexity, it remains largely unknown how plant cells monitor and ensure the homeostasis of auxin responses. PIN-LIKES (PILS) intracellular auxin transpo...

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Autores principales: Feraru, Elena, Feraru, Mugurel I., Moulinier-Anzola, Jeanette, Schwihla, Maximilian, Ferreira Da Silva Santos, Jonathan, Sun, Lin, Waidmann, Sascha, Korbei, Barbara, Kleine-Vehn, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340555/
https://www.ncbi.nlm.nih.gov/pubmed/35819066
http://dx.doi.org/10.1242/dev.200929
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author Feraru, Elena
Feraru, Mugurel I.
Moulinier-Anzola, Jeanette
Schwihla, Maximilian
Ferreira Da Silva Santos, Jonathan
Sun, Lin
Waidmann, Sascha
Korbei, Barbara
Kleine-Vehn, Jürgen
author_facet Feraru, Elena
Feraru, Mugurel I.
Moulinier-Anzola, Jeanette
Schwihla, Maximilian
Ferreira Da Silva Santos, Jonathan
Sun, Lin
Waidmann, Sascha
Korbei, Barbara
Kleine-Vehn, Jürgen
author_sort Feraru, Elena
collection PubMed
description Multiple internal and external signals modulate the metabolism, intercellular transport and signaling of the phytohormone auxin. Considering this complexity, it remains largely unknown how plant cells monitor and ensure the homeostasis of auxin responses. PIN-LIKES (PILS) intracellular auxin transport facilitators at the endoplasmic reticulum are suitable candidates to buffer cellular auxin responses because they limit nuclear abundance and signaling of auxin. We used forward genetics to identify gloomy and shiny pils (gasp) mutants that define the PILS6 protein abundance in a post-translational manner. Here, we show that GASP1 encodes an uncharacterized RING/U-box superfamily protein that impacts on auxin signaling output. The low auxin signaling in gasp1 mutants correlates with reduced abundance of PILS5 and PILS6 proteins. Mechanistically, we show that high and low auxin conditions increase and reduce PILS6 protein levels, respectively. Accordingly, non-optimum auxin concentrations are buffered by alterations in PILS6 abundance, consequently leading to homeostatic auxin output regulation. We envision that this feedback mechanism provides robustness to auxin-dependent plant development.
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spelling pubmed-93405552022-08-03 PILS proteins provide a homeostatic feedback on auxin signaling output Feraru, Elena Feraru, Mugurel I. Moulinier-Anzola, Jeanette Schwihla, Maximilian Ferreira Da Silva Santos, Jonathan Sun, Lin Waidmann, Sascha Korbei, Barbara Kleine-Vehn, Jürgen Development Research Article Multiple internal and external signals modulate the metabolism, intercellular transport and signaling of the phytohormone auxin. Considering this complexity, it remains largely unknown how plant cells monitor and ensure the homeostasis of auxin responses. PIN-LIKES (PILS) intracellular auxin transport facilitators at the endoplasmic reticulum are suitable candidates to buffer cellular auxin responses because they limit nuclear abundance and signaling of auxin. We used forward genetics to identify gloomy and shiny pils (gasp) mutants that define the PILS6 protein abundance in a post-translational manner. Here, we show that GASP1 encodes an uncharacterized RING/U-box superfamily protein that impacts on auxin signaling output. The low auxin signaling in gasp1 mutants correlates with reduced abundance of PILS5 and PILS6 proteins. Mechanistically, we show that high and low auxin conditions increase and reduce PILS6 protein levels, respectively. Accordingly, non-optimum auxin concentrations are buffered by alterations in PILS6 abundance, consequently leading to homeostatic auxin output regulation. We envision that this feedback mechanism provides robustness to auxin-dependent plant development. The Company of Biologists Ltd 2022-07-12 /pmc/articles/PMC9340555/ /pubmed/35819066 http://dx.doi.org/10.1242/dev.200929 Text en © 2022. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Feraru, Elena
Feraru, Mugurel I.
Moulinier-Anzola, Jeanette
Schwihla, Maximilian
Ferreira Da Silva Santos, Jonathan
Sun, Lin
Waidmann, Sascha
Korbei, Barbara
Kleine-Vehn, Jürgen
PILS proteins provide a homeostatic feedback on auxin signaling output
title PILS proteins provide a homeostatic feedback on auxin signaling output
title_full PILS proteins provide a homeostatic feedback on auxin signaling output
title_fullStr PILS proteins provide a homeostatic feedback on auxin signaling output
title_full_unstemmed PILS proteins provide a homeostatic feedback on auxin signaling output
title_short PILS proteins provide a homeostatic feedback on auxin signaling output
title_sort pils proteins provide a homeostatic feedback on auxin signaling output
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340555/
https://www.ncbi.nlm.nih.gov/pubmed/35819066
http://dx.doi.org/10.1242/dev.200929
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