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ARL2 is required for homologous recombination repair and colon cancer stem cell survival

ARL2 regulates the dynamics of cytological components and is highly expressed in colon cancer tissues. Here, we report novel roles of ARL2 in the cell nucleus and colon cancer stem cells (CSCs). ARL2 is expressed at relatively low levels in K‐RAS active colon cancer cells, but its expression is indu...

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Autores principales: Lee, Hani, Choi, SeokGyeong, Ha, Sojung, Yoon, Sukjoon, Kim, Woo‐Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340879/
https://www.ncbi.nlm.nih.gov/pubmed/35567502
http://dx.doi.org/10.1002/2211-5463.13438
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author Lee, Hani
Choi, SeokGyeong
Ha, Sojung
Yoon, Sukjoon
Kim, Woo‐Young
author_facet Lee, Hani
Choi, SeokGyeong
Ha, Sojung
Yoon, Sukjoon
Kim, Woo‐Young
author_sort Lee, Hani
collection PubMed
description ARL2 regulates the dynamics of cytological components and is highly expressed in colon cancer tissues. Here, we report novel roles of ARL2 in the cell nucleus and colon cancer stem cells (CSCs). ARL2 is expressed at relatively low levels in K‐RAS active colon cancer cells, but its expression is induced in CSCs. Depletion of ARL2 results in M phase arrest exclusively in non‐CSC cultured cells; in addition, DNA break stress accumulates in CSCs leading to apoptosis. ARL2 expression is positively associated with the expression of all six RAD51 family genes, which are essential for homologous recombination repair (HRR). Furthermore, ARL2 is required for HRR and detected within chromatin compartments. These results demonstrate the requirement of ARL2 in colon CSC maintenance, which possibly occurs through mediating double‐strand break DNA repair in the nucleus.
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spelling pubmed-93408792022-08-02 ARL2 is required for homologous recombination repair and colon cancer stem cell survival Lee, Hani Choi, SeokGyeong Ha, Sojung Yoon, Sukjoon Kim, Woo‐Young FEBS Open Bio Research Articles ARL2 regulates the dynamics of cytological components and is highly expressed in colon cancer tissues. Here, we report novel roles of ARL2 in the cell nucleus and colon cancer stem cells (CSCs). ARL2 is expressed at relatively low levels in K‐RAS active colon cancer cells, but its expression is induced in CSCs. Depletion of ARL2 results in M phase arrest exclusively in non‐CSC cultured cells; in addition, DNA break stress accumulates in CSCs leading to apoptosis. ARL2 expression is positively associated with the expression of all six RAD51 family genes, which are essential for homologous recombination repair (HRR). Furthermore, ARL2 is required for HRR and detected within chromatin compartments. These results demonstrate the requirement of ARL2 in colon CSC maintenance, which possibly occurs through mediating double‐strand break DNA repair in the nucleus. John Wiley and Sons Inc. 2022-05-24 /pmc/articles/PMC9340879/ /pubmed/35567502 http://dx.doi.org/10.1002/2211-5463.13438 Text en © 2022 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Lee, Hani
Choi, SeokGyeong
Ha, Sojung
Yoon, Sukjoon
Kim, Woo‐Young
ARL2 is required for homologous recombination repair and colon cancer stem cell survival
title ARL2 is required for homologous recombination repair and colon cancer stem cell survival
title_full ARL2 is required for homologous recombination repair and colon cancer stem cell survival
title_fullStr ARL2 is required for homologous recombination repair and colon cancer stem cell survival
title_full_unstemmed ARL2 is required for homologous recombination repair and colon cancer stem cell survival
title_short ARL2 is required for homologous recombination repair and colon cancer stem cell survival
title_sort arl2 is required for homologous recombination repair and colon cancer stem cell survival
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340879/
https://www.ncbi.nlm.nih.gov/pubmed/35567502
http://dx.doi.org/10.1002/2211-5463.13438
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