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Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers
Lymphatic metastasis influences clinical treatment and prognosis of patients with non-small-cell lung cancer (NSCLC). There is an urgency to understand the molecular features and mechanisms of lymph node metastasis. We analyzed the molecular features on pairs of the primary tumor and lymphatic metas...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341247/ https://www.ncbi.nlm.nih.gov/pubmed/35923575 http://dx.doi.org/10.3389/fbioe.2022.909388 |
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author | Guo, Nannan Chen, Yuanyuan Jing, Zhongying Liu, Siyao Su, Junyan Li, Ruilin Duan, Xiaohong Chen, Zhigong Chen, Ping Yin, Rongjiang Li, Shaojun Tang, Jian |
author_facet | Guo, Nannan Chen, Yuanyuan Jing, Zhongying Liu, Siyao Su, Junyan Li, Ruilin Duan, Xiaohong Chen, Zhigong Chen, Ping Yin, Rongjiang Li, Shaojun Tang, Jian |
author_sort | Guo, Nannan |
collection | PubMed |
description | Lymphatic metastasis influences clinical treatment and prognosis of patients with non-small-cell lung cancer (NSCLC). There is an urgency to understand the molecular features and mechanisms of lymph node metastasis. We analyzed the molecular features on pairs of the primary tumor and lymphatic metastasis tissue samples from 15 NSCLC patients using targeted next-generation sequencing. The potential metastasis-related genes were screened from our cohort based on cancer cell fraction. After filtering with gene functions, candidate metastasis-related events were validated in the MSK cohort with Fisher’s exact test. The molecular signature and tumor mutational burden were similar in paired samples, and the average mutational concordance was 42.0% ± 28.9%. Its metastatic mechanism is potentially a linear progression based on the metastatic seeding theory. Furthermore, mutated ataxia telangiectasia mutated and Rad3-related (ATR) and tet methylcytosine dioxygenase 2 (TET2) genes were significantly enriched in lymphatic metastases (p ≤ 0.05). Alterations in these two genes could be considered metastasis-related driving events. Mutated ATR and TET2 might play an active role in the metastasis of lymph nodes with NSCLC. More case enrollment and long-term follow-up will further verify the clinical significance of these two genes. |
format | Online Article Text |
id | pubmed-9341247 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93412472022-08-02 Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers Guo, Nannan Chen, Yuanyuan Jing, Zhongying Liu, Siyao Su, Junyan Li, Ruilin Duan, Xiaohong Chen, Zhigong Chen, Ping Yin, Rongjiang Li, Shaojun Tang, Jian Front Bioeng Biotechnol Bioengineering and Biotechnology Lymphatic metastasis influences clinical treatment and prognosis of patients with non-small-cell lung cancer (NSCLC). There is an urgency to understand the molecular features and mechanisms of lymph node metastasis. We analyzed the molecular features on pairs of the primary tumor and lymphatic metastasis tissue samples from 15 NSCLC patients using targeted next-generation sequencing. The potential metastasis-related genes were screened from our cohort based on cancer cell fraction. After filtering with gene functions, candidate metastasis-related events were validated in the MSK cohort with Fisher’s exact test. The molecular signature and tumor mutational burden were similar in paired samples, and the average mutational concordance was 42.0% ± 28.9%. Its metastatic mechanism is potentially a linear progression based on the metastatic seeding theory. Furthermore, mutated ataxia telangiectasia mutated and Rad3-related (ATR) and tet methylcytosine dioxygenase 2 (TET2) genes were significantly enriched in lymphatic metastases (p ≤ 0.05). Alterations in these two genes could be considered metastasis-related driving events. Mutated ATR and TET2 might play an active role in the metastasis of lymph nodes with NSCLC. More case enrollment and long-term follow-up will further verify the clinical significance of these two genes. Frontiers Media S.A. 2022-07-18 /pmc/articles/PMC9341247/ /pubmed/35923575 http://dx.doi.org/10.3389/fbioe.2022.909388 Text en Copyright © 2022 Guo, Chen, Jing, Liu, Su, Li, Duan, Chen, Chen, Yin, Li and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Guo, Nannan Chen, Yuanyuan Jing, Zhongying Liu, Siyao Su, Junyan Li, Ruilin Duan, Xiaohong Chen, Zhigong Chen, Ping Yin, Rongjiang Li, Shaojun Tang, Jian Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers |
title | Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers |
title_full | Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers |
title_fullStr | Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers |
title_full_unstemmed | Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers |
title_short | Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers |
title_sort | molecular features in lymphatic metastases reflect the metastasis mechanism of lymph nodes with non-small-cell lung cancers |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341247/ https://www.ncbi.nlm.nih.gov/pubmed/35923575 http://dx.doi.org/10.3389/fbioe.2022.909388 |
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