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Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers

Lymphatic metastasis influences clinical treatment and prognosis of patients with non-small-cell lung cancer (NSCLC). There is an urgency to understand the molecular features and mechanisms of lymph node metastasis. We analyzed the molecular features on pairs of the primary tumor and lymphatic metas...

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Autores principales: Guo, Nannan, Chen, Yuanyuan, Jing, Zhongying, Liu, Siyao, Su, Junyan, Li, Ruilin, Duan, Xiaohong, Chen, Zhigong, Chen, Ping, Yin, Rongjiang, Li, Shaojun, Tang, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341247/
https://www.ncbi.nlm.nih.gov/pubmed/35923575
http://dx.doi.org/10.3389/fbioe.2022.909388
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author Guo, Nannan
Chen, Yuanyuan
Jing, Zhongying
Liu, Siyao
Su, Junyan
Li, Ruilin
Duan, Xiaohong
Chen, Zhigong
Chen, Ping
Yin, Rongjiang
Li, Shaojun
Tang, Jian
author_facet Guo, Nannan
Chen, Yuanyuan
Jing, Zhongying
Liu, Siyao
Su, Junyan
Li, Ruilin
Duan, Xiaohong
Chen, Zhigong
Chen, Ping
Yin, Rongjiang
Li, Shaojun
Tang, Jian
author_sort Guo, Nannan
collection PubMed
description Lymphatic metastasis influences clinical treatment and prognosis of patients with non-small-cell lung cancer (NSCLC). There is an urgency to understand the molecular features and mechanisms of lymph node metastasis. We analyzed the molecular features on pairs of the primary tumor and lymphatic metastasis tissue samples from 15 NSCLC patients using targeted next-generation sequencing. The potential metastasis-related genes were screened from our cohort based on cancer cell fraction. After filtering with gene functions, candidate metastasis-related events were validated in the MSK cohort with Fisher’s exact test. The molecular signature and tumor mutational burden were similar in paired samples, and the average mutational concordance was 42.0% ± 28.9%. Its metastatic mechanism is potentially a linear progression based on the metastatic seeding theory. Furthermore, mutated ataxia telangiectasia mutated and Rad3-related (ATR) and tet methylcytosine dioxygenase 2 (TET2) genes were significantly enriched in lymphatic metastases (p ≤ 0.05). Alterations in these two genes could be considered metastasis-related driving events. Mutated ATR and TET2 might play an active role in the metastasis of lymph nodes with NSCLC. More case enrollment and long-term follow-up will further verify the clinical significance of these two genes.
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spelling pubmed-93412472022-08-02 Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers Guo, Nannan Chen, Yuanyuan Jing, Zhongying Liu, Siyao Su, Junyan Li, Ruilin Duan, Xiaohong Chen, Zhigong Chen, Ping Yin, Rongjiang Li, Shaojun Tang, Jian Front Bioeng Biotechnol Bioengineering and Biotechnology Lymphatic metastasis influences clinical treatment and prognosis of patients with non-small-cell lung cancer (NSCLC). There is an urgency to understand the molecular features and mechanisms of lymph node metastasis. We analyzed the molecular features on pairs of the primary tumor and lymphatic metastasis tissue samples from 15 NSCLC patients using targeted next-generation sequencing. The potential metastasis-related genes were screened from our cohort based on cancer cell fraction. After filtering with gene functions, candidate metastasis-related events were validated in the MSK cohort with Fisher’s exact test. The molecular signature and tumor mutational burden were similar in paired samples, and the average mutational concordance was 42.0% ± 28.9%. Its metastatic mechanism is potentially a linear progression based on the metastatic seeding theory. Furthermore, mutated ataxia telangiectasia mutated and Rad3-related (ATR) and tet methylcytosine dioxygenase 2 (TET2) genes were significantly enriched in lymphatic metastases (p ≤ 0.05). Alterations in these two genes could be considered metastasis-related driving events. Mutated ATR and TET2 might play an active role in the metastasis of lymph nodes with NSCLC. More case enrollment and long-term follow-up will further verify the clinical significance of these two genes. Frontiers Media S.A. 2022-07-18 /pmc/articles/PMC9341247/ /pubmed/35923575 http://dx.doi.org/10.3389/fbioe.2022.909388 Text en Copyright © 2022 Guo, Chen, Jing, Liu, Su, Li, Duan, Chen, Chen, Yin, Li and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Guo, Nannan
Chen, Yuanyuan
Jing, Zhongying
Liu, Siyao
Su, Junyan
Li, Ruilin
Duan, Xiaohong
Chen, Zhigong
Chen, Ping
Yin, Rongjiang
Li, Shaojun
Tang, Jian
Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers
title Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers
title_full Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers
title_fullStr Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers
title_full_unstemmed Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers
title_short Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers
title_sort molecular features in lymphatic metastases reflect the metastasis mechanism of lymph nodes with non-small-cell lung cancers
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341247/
https://www.ncbi.nlm.nih.gov/pubmed/35923575
http://dx.doi.org/10.3389/fbioe.2022.909388
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