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Standardization of the FAO/IAEA Flight Test for Quality Control of Sterile Mosquitoes
Successful implementation of the sterile insect technique (SIT) against Aedes aegypti and Aedes albopictus relies on maintaining a consistent release of high-quality sterile males. Affordable, rapid, practical quality control tools based on the male’s flight ability (ability to escape from a flight...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341283/ https://www.ncbi.nlm.nih.gov/pubmed/35923573 http://dx.doi.org/10.3389/fbioe.2022.876675 |
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author | Maïga, Hamidou Lu, Deng Mamai, Wadaka Bimbilé Somda, Nanwintoum Séverin Wallner, Thomas Bakhoum, Mame Thierno Bueno Masso, Odet Martina, Claudia Kotla, Simran Singh Yamada, Hanano Salvador Herranz, Gustavo Argiles Herrero, Rafael Chong, Chee Seng Tan, Cheong Huat Bouyer, Jeremy |
author_facet | Maïga, Hamidou Lu, Deng Mamai, Wadaka Bimbilé Somda, Nanwintoum Séverin Wallner, Thomas Bakhoum, Mame Thierno Bueno Masso, Odet Martina, Claudia Kotla, Simran Singh Yamada, Hanano Salvador Herranz, Gustavo Argiles Herrero, Rafael Chong, Chee Seng Tan, Cheong Huat Bouyer, Jeremy |
author_sort | Maïga, Hamidou |
collection | PubMed |
description | Successful implementation of the sterile insect technique (SIT) against Aedes aegypti and Aedes albopictus relies on maintaining a consistent release of high-quality sterile males. Affordable, rapid, practical quality control tools based on the male’s flight ability (ability to escape from a flight device) may contribute to meeting this requirement. Therefore, this study aims to standardize the use of the original FAO/IAEA rapid quality control flight test device (FTD) (version 1.0), while improving handling conditions and reducing the device’s overall cost by assessing factors that could impact the subsequent flight ability of Aedes mosquitoes. The new FTD (version 1.1) is easier to use. The most important factors affecting escape rates were found to be tube color (or “shade”), the combined use of a lure and fan, mosquito species, and mosquito age and density (25; 50; 75; 100 males). Other factors measured but found to be less important were the duration of the test (30, 60, 90, 120 min), fan speed (normal 3000 rpm vs. high 6000 rpm), and mosquito strain origin. In addition, a cheaper version of the FTD (version 2.0) that holds eight individual tubes instead of 40 was designed and successfully validated against the new FTD (version 1.1). It was sensitive enough to distinguish between the effects of cold stress and high irradiation dose. Therefore, the eight-tube FTD may be used to assess Aedes’ flight ability. This study demonstrated that the new designs (versions 1.1 and 2.0) of the FTD could be used for standard routine quality assessments of Aedes mosquitoes required for an SIT and other male release-based programs. |
format | Online Article Text |
id | pubmed-9341283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93412832022-08-02 Standardization of the FAO/IAEA Flight Test for Quality Control of Sterile Mosquitoes Maïga, Hamidou Lu, Deng Mamai, Wadaka Bimbilé Somda, Nanwintoum Séverin Wallner, Thomas Bakhoum, Mame Thierno Bueno Masso, Odet Martina, Claudia Kotla, Simran Singh Yamada, Hanano Salvador Herranz, Gustavo Argiles Herrero, Rafael Chong, Chee Seng Tan, Cheong Huat Bouyer, Jeremy Front Bioeng Biotechnol Bioengineering and Biotechnology Successful implementation of the sterile insect technique (SIT) against Aedes aegypti and Aedes albopictus relies on maintaining a consistent release of high-quality sterile males. Affordable, rapid, practical quality control tools based on the male’s flight ability (ability to escape from a flight device) may contribute to meeting this requirement. Therefore, this study aims to standardize the use of the original FAO/IAEA rapid quality control flight test device (FTD) (version 1.0), while improving handling conditions and reducing the device’s overall cost by assessing factors that could impact the subsequent flight ability of Aedes mosquitoes. The new FTD (version 1.1) is easier to use. The most important factors affecting escape rates were found to be tube color (or “shade”), the combined use of a lure and fan, mosquito species, and mosquito age and density (25; 50; 75; 100 males). Other factors measured but found to be less important were the duration of the test (30, 60, 90, 120 min), fan speed (normal 3000 rpm vs. high 6000 rpm), and mosquito strain origin. In addition, a cheaper version of the FTD (version 2.0) that holds eight individual tubes instead of 40 was designed and successfully validated against the new FTD (version 1.1). It was sensitive enough to distinguish between the effects of cold stress and high irradiation dose. Therefore, the eight-tube FTD may be used to assess Aedes’ flight ability. This study demonstrated that the new designs (versions 1.1 and 2.0) of the FTD could be used for standard routine quality assessments of Aedes mosquitoes required for an SIT and other male release-based programs. Frontiers Media S.A. 2022-07-18 /pmc/articles/PMC9341283/ /pubmed/35923573 http://dx.doi.org/10.3389/fbioe.2022.876675 Text en Copyright © 2022 Maïga, Lu, Mamai, Bimbilé Somda, Wallner, Bakhoum, Bueno Masso, Martina, Kotla, Yamada, Salvador Herranz, Argiles Herrero, Chong, Tan and Bouyer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Maïga, Hamidou Lu, Deng Mamai, Wadaka Bimbilé Somda, Nanwintoum Séverin Wallner, Thomas Bakhoum, Mame Thierno Bueno Masso, Odet Martina, Claudia Kotla, Simran Singh Yamada, Hanano Salvador Herranz, Gustavo Argiles Herrero, Rafael Chong, Chee Seng Tan, Cheong Huat Bouyer, Jeremy Standardization of the FAO/IAEA Flight Test for Quality Control of Sterile Mosquitoes |
title | Standardization of the FAO/IAEA Flight Test for Quality Control of Sterile Mosquitoes |
title_full | Standardization of the FAO/IAEA Flight Test for Quality Control of Sterile Mosquitoes |
title_fullStr | Standardization of the FAO/IAEA Flight Test for Quality Control of Sterile Mosquitoes |
title_full_unstemmed | Standardization of the FAO/IAEA Flight Test for Quality Control of Sterile Mosquitoes |
title_short | Standardization of the FAO/IAEA Flight Test for Quality Control of Sterile Mosquitoes |
title_sort | standardization of the fao/iaea flight test for quality control of sterile mosquitoes |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341283/ https://www.ncbi.nlm.nih.gov/pubmed/35923573 http://dx.doi.org/10.3389/fbioe.2022.876675 |
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