Cargando…

Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees

Paenibacillus larvae is the etiological agent of American Foulbrood (AFB), a highly contagious and worldwide spread bacterial disease that affects honeybee brood. In this study, all complete P. larvae genomes available on the NCBI database were analyzed in order to detect presence of prophages using...

Descripción completa

Detalles Bibliográficos
Autores principales: Ribeiro, Henrique G., Nilsson, Anna, Melo, Luís D. R., Oliveira, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341999/
https://www.ncbi.nlm.nih.gov/pubmed/35923395
http://dx.doi.org/10.3389/fmicb.2022.903861
_version_ 1784760731062763520
author Ribeiro, Henrique G.
Nilsson, Anna
Melo, Luís D. R.
Oliveira, Ana
author_facet Ribeiro, Henrique G.
Nilsson, Anna
Melo, Luís D. R.
Oliveira, Ana
author_sort Ribeiro, Henrique G.
collection PubMed
description Paenibacillus larvae is the etiological agent of American Foulbrood (AFB), a highly contagious and worldwide spread bacterial disease that affects honeybee brood. In this study, all complete P. larvae genomes available on the NCBI database were analyzed in order to detect presence of prophages using the PHASTER software. A total of 55 intact prophages were identified in 11 P. larvae genomes (5.0 ± 2.3 per genome) and were further investigated for the presence of genes encoding relevant traits related to P. larvae. A closer look at the prophage genomes revealed the presence of several putative genes such as metabolic and antimicrobial resistance genes, toxins or bacteriocins, potentially influencing host performance. Some of the coding DNA sequences (CDS) were present in all ERIC-genotypes, while others were only found in a specific genotype. While CDS encoding toxins and antitoxins such as HicB and MazE were found in prophages of all bacterial genotypes, others, from the same category, were provided by prophages particularly to ERIC I (enhancin-like toxin), ERIC II (antitoxin SocA) and ERIC V strains (subunit of Panton-Valentine leukocidin system (PVL) LukF-PV). This is the first in-depth analysis of P. larvae prophages. It provides better knowledge on their impact in the evolution of virulence and fitness of P. larvae, by discovering new features assigned by the viruses.
format Online
Article
Text
id pubmed-9341999
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93419992022-08-02 Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees Ribeiro, Henrique G. Nilsson, Anna Melo, Luís D. R. Oliveira, Ana Front Microbiol Microbiology Paenibacillus larvae is the etiological agent of American Foulbrood (AFB), a highly contagious and worldwide spread bacterial disease that affects honeybee brood. In this study, all complete P. larvae genomes available on the NCBI database were analyzed in order to detect presence of prophages using the PHASTER software. A total of 55 intact prophages were identified in 11 P. larvae genomes (5.0 ± 2.3 per genome) and were further investigated for the presence of genes encoding relevant traits related to P. larvae. A closer look at the prophage genomes revealed the presence of several putative genes such as metabolic and antimicrobial resistance genes, toxins or bacteriocins, potentially influencing host performance. Some of the coding DNA sequences (CDS) were present in all ERIC-genotypes, while others were only found in a specific genotype. While CDS encoding toxins and antitoxins such as HicB and MazE were found in prophages of all bacterial genotypes, others, from the same category, were provided by prophages particularly to ERIC I (enhancin-like toxin), ERIC II (antitoxin SocA) and ERIC V strains (subunit of Panton-Valentine leukocidin system (PVL) LukF-PV). This is the first in-depth analysis of P. larvae prophages. It provides better knowledge on their impact in the evolution of virulence and fitness of P. larvae, by discovering new features assigned by the viruses. Frontiers Media S.A. 2022-07-15 /pmc/articles/PMC9341999/ /pubmed/35923395 http://dx.doi.org/10.3389/fmicb.2022.903861 Text en Copyright © 2022 Ribeiro, Nilsson, Melo and Oliveira. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ribeiro, Henrique G.
Nilsson, Anna
Melo, Luís D. R.
Oliveira, Ana
Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees
title Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees
title_full Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees
title_fullStr Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees
title_full_unstemmed Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees
title_short Analysis of intact prophages in genomes of Paenibacillus larvae: An important pathogen for bees
title_sort analysis of intact prophages in genomes of paenibacillus larvae: an important pathogen for bees
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341999/
https://www.ncbi.nlm.nih.gov/pubmed/35923395
http://dx.doi.org/10.3389/fmicb.2022.903861
work_keys_str_mv AT ribeirohenriqueg analysisofintactprophagesingenomesofpaenibacilluslarvaeanimportantpathogenforbees
AT nilssonanna analysisofintactprophagesingenomesofpaenibacilluslarvaeanimportantpathogenforbees
AT meloluisdr analysisofintactprophagesingenomesofpaenibacilluslarvaeanimportantpathogenforbees
AT oliveiraana analysisofintactprophagesingenomesofpaenibacilluslarvaeanimportantpathogenforbees