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Application of developmental regulators to improve in planta or in vitro transformation in plants

Plant genetic transformation is a crucial step for applying biotechnology such as genome editing to basic and applied plant science research. Its success primarily relies on the efficiency of gene delivery into plant cells and the ability to regenerate transgenic plants. In this study, we have exami...

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Autores principales: Lian, Zhaoyuan, Nguyen, Chi Dinh, Liu, Li, Wang, Guiluan, Chen, Jianjun, Wang, Songhu, Yi, Ganjun, Wilson, Sandra, Ozias‐Akins, Peggy, Gong, Haijun, Huo, Heqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342618/
https://www.ncbi.nlm.nih.gov/pubmed/35524453
http://dx.doi.org/10.1111/pbi.13837
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author Lian, Zhaoyuan
Nguyen, Chi Dinh
Liu, Li
Wang, Guiluan
Chen, Jianjun
Wang, Songhu
Yi, Ganjun
Wilson, Sandra
Ozias‐Akins, Peggy
Gong, Haijun
Huo, Heqiang
author_facet Lian, Zhaoyuan
Nguyen, Chi Dinh
Liu, Li
Wang, Guiluan
Chen, Jianjun
Wang, Songhu
Yi, Ganjun
Wilson, Sandra
Ozias‐Akins, Peggy
Gong, Haijun
Huo, Heqiang
author_sort Lian, Zhaoyuan
collection PubMed
description Plant genetic transformation is a crucial step for applying biotechnology such as genome editing to basic and applied plant science research. Its success primarily relies on the efficiency of gene delivery into plant cells and the ability to regenerate transgenic plants. In this study, we have examined the effect of several developmental regulators (DRs), including PLETHORA (PLT5), WOUND INDUCED DEDIFFERENTIATION 1 (WIND1), ENHANCED SHOOT REGENERATION (ESR1), WUSHEL (WUS) and a fusion of WUS and BABY‐BOOM (WUS‐P2A‐BBM), on in planta transformation through injection of Agrobacterium tumefaciens in snapdragons (Antirrhinum majus). The results showed that PLT5, WIND1 and WUS promoted in planta transformation of snapdragons. An additional test of these three DRs on tomato (Solanum lycopersicum) further demonstrated that the highest in planta transformation efficiency was observed from PLT5. PLT5 promoted calli formation and regeneration of transformed shoots at the wound positions of aerial stems, and the transgene was stably inherited to the next generation in snapdragons. Additionally, PLT5 significantly improved the shoot regeneration and transformation in two Brassica cabbage varieties (Brassica rapa) and promoted the formation of transgenic calli and somatic embryos in sweet pepper (Capsicum annum) through in vitro tissue culture. Despite some morphological alternations, viable seeds were produced from the transgenic Bok choy and snapdragons. Our results have demonstrated that manipulation of PLT5 could be an effective approach for improving in planta and in vitro transformation efficiency, and such a transformation system could be used to facilitate the application of genome editing or other plant biotechnology application in modern agriculture.
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spelling pubmed-93426182022-08-03 Application of developmental regulators to improve in planta or in vitro transformation in plants Lian, Zhaoyuan Nguyen, Chi Dinh Liu, Li Wang, Guiluan Chen, Jianjun Wang, Songhu Yi, Ganjun Wilson, Sandra Ozias‐Akins, Peggy Gong, Haijun Huo, Heqiang Plant Biotechnol J Research Articles Plant genetic transformation is a crucial step for applying biotechnology such as genome editing to basic and applied plant science research. Its success primarily relies on the efficiency of gene delivery into plant cells and the ability to regenerate transgenic plants. In this study, we have examined the effect of several developmental regulators (DRs), including PLETHORA (PLT5), WOUND INDUCED DEDIFFERENTIATION 1 (WIND1), ENHANCED SHOOT REGENERATION (ESR1), WUSHEL (WUS) and a fusion of WUS and BABY‐BOOM (WUS‐P2A‐BBM), on in planta transformation through injection of Agrobacterium tumefaciens in snapdragons (Antirrhinum majus). The results showed that PLT5, WIND1 and WUS promoted in planta transformation of snapdragons. An additional test of these three DRs on tomato (Solanum lycopersicum) further demonstrated that the highest in planta transformation efficiency was observed from PLT5. PLT5 promoted calli formation and regeneration of transformed shoots at the wound positions of aerial stems, and the transgene was stably inherited to the next generation in snapdragons. Additionally, PLT5 significantly improved the shoot regeneration and transformation in two Brassica cabbage varieties (Brassica rapa) and promoted the formation of transgenic calli and somatic embryos in sweet pepper (Capsicum annum) through in vitro tissue culture. Despite some morphological alternations, viable seeds were produced from the transgenic Bok choy and snapdragons. Our results have demonstrated that manipulation of PLT5 could be an effective approach for improving in planta and in vitro transformation efficiency, and such a transformation system could be used to facilitate the application of genome editing or other plant biotechnology application in modern agriculture. John Wiley and Sons Inc. 2022-05-25 2022-08 /pmc/articles/PMC9342618/ /pubmed/35524453 http://dx.doi.org/10.1111/pbi.13837 Text en © 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Lian, Zhaoyuan
Nguyen, Chi Dinh
Liu, Li
Wang, Guiluan
Chen, Jianjun
Wang, Songhu
Yi, Ganjun
Wilson, Sandra
Ozias‐Akins, Peggy
Gong, Haijun
Huo, Heqiang
Application of developmental regulators to improve in planta or in vitro transformation in plants
title Application of developmental regulators to improve in planta or in vitro transformation in plants
title_full Application of developmental regulators to improve in planta or in vitro transformation in plants
title_fullStr Application of developmental regulators to improve in planta or in vitro transformation in plants
title_full_unstemmed Application of developmental regulators to improve in planta or in vitro transformation in plants
title_short Application of developmental regulators to improve in planta or in vitro transformation in plants
title_sort application of developmental regulators to improve in planta or in vitro transformation in plants
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342618/
https://www.ncbi.nlm.nih.gov/pubmed/35524453
http://dx.doi.org/10.1111/pbi.13837
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