Cargando…
SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato
MiRNAs are important posttranscriptional regulators of plant development. Many miRNAs, such as the conserved miR164 species, are encoded by families of MIRNA genes, but the specific roles of individual MIRNA genes are largely undefined. Here, we characterize the functions and regulatory mechanisms o...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342619/ https://www.ncbi.nlm.nih.gov/pubmed/35403821 http://dx.doi.org/10.1111/pbi.13824 |
_version_ | 1784760867590504448 |
---|---|
author | Lin, Dongbo Zhu, Xiaoen Qi, Binglin Gao, Zhong Tian, Peng Li, Ziwei Lin, Zeteng Zhang, Yongxia Huang, Tengbo |
author_facet | Lin, Dongbo Zhu, Xiaoen Qi, Binglin Gao, Zhong Tian, Peng Li, Ziwei Lin, Zeteng Zhang, Yongxia Huang, Tengbo |
author_sort | Lin, Dongbo |
collection | PubMed |
description | MiRNAs are important posttranscriptional regulators of plant development. Many miRNAs, such as the conserved miR164 species, are encoded by families of MIRNA genes, but the specific roles of individual MIRNA genes are largely undefined. Here, we characterize the functions and regulatory mechanisms of SlMIR164A, one of the primary genes of Sly‐miR164, in tomato. We show that SlMIR164A is preferentially expressed at late stages of fruit development and plays a vital role in controlling fruit ripening and quality. Loss of function of SlMIR164A by CRISPR/Cas9‐mediated mutagenesis results in accelerated fruit ripening and enhanced chloroplast development, which leads to altered sugar and organic acid contents and affects the nutritional quality of fruits. We also show that SlMIR164A modulates fruit ripening and quality through specific target genes, SlNAM2 and SlNAM3, which control key regulators of chloroplast function and fruit ripening processes. MIR164 genes have been shown to play conserved roles in regulating organ ageing, such as leaf senescence and fruit ripening, in a variety of plants, but whether and how their family members in tomato exert the same function remain to be elucidated. Our results reveal a previously undiscovered role of SlMIR164A in ripening control, which will further our understanding of the actions of MIR164 family, as well as the mechanisms of fruit ripening and quality control in tomato. Moreover, as loss of SlMIR164A exhibits minor impacts on organ morphology, our results can be leveraged in tomato breeding for specific manipulation of fruit ripening and quality to facilitate tomato improvement in agriculture. |
format | Online Article Text |
id | pubmed-9342619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93426192022-08-03 SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato Lin, Dongbo Zhu, Xiaoen Qi, Binglin Gao, Zhong Tian, Peng Li, Ziwei Lin, Zeteng Zhang, Yongxia Huang, Tengbo Plant Biotechnol J Research Articles MiRNAs are important posttranscriptional regulators of plant development. Many miRNAs, such as the conserved miR164 species, are encoded by families of MIRNA genes, but the specific roles of individual MIRNA genes are largely undefined. Here, we characterize the functions and regulatory mechanisms of SlMIR164A, one of the primary genes of Sly‐miR164, in tomato. We show that SlMIR164A is preferentially expressed at late stages of fruit development and plays a vital role in controlling fruit ripening and quality. Loss of function of SlMIR164A by CRISPR/Cas9‐mediated mutagenesis results in accelerated fruit ripening and enhanced chloroplast development, which leads to altered sugar and organic acid contents and affects the nutritional quality of fruits. We also show that SlMIR164A modulates fruit ripening and quality through specific target genes, SlNAM2 and SlNAM3, which control key regulators of chloroplast function and fruit ripening processes. MIR164 genes have been shown to play conserved roles in regulating organ ageing, such as leaf senescence and fruit ripening, in a variety of plants, but whether and how their family members in tomato exert the same function remain to be elucidated. Our results reveal a previously undiscovered role of SlMIR164A in ripening control, which will further our understanding of the actions of MIR164 family, as well as the mechanisms of fruit ripening and quality control in tomato. Moreover, as loss of SlMIR164A exhibits minor impacts on organ morphology, our results can be leveraged in tomato breeding for specific manipulation of fruit ripening and quality to facilitate tomato improvement in agriculture. John Wiley and Sons Inc. 2022-04-28 2022-08 /pmc/articles/PMC9342619/ /pubmed/35403821 http://dx.doi.org/10.1111/pbi.13824 Text en © 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Lin, Dongbo Zhu, Xiaoen Qi, Binglin Gao, Zhong Tian, Peng Li, Ziwei Lin, Zeteng Zhang, Yongxia Huang, Tengbo SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato |
title |
SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato |
title_full |
SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato |
title_fullStr |
SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato |
title_full_unstemmed |
SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato |
title_short |
SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato |
title_sort | slmir164a regulates fruit ripening and quality by controlling slnam2 and slnam3 in tomato |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342619/ https://www.ncbi.nlm.nih.gov/pubmed/35403821 http://dx.doi.org/10.1111/pbi.13824 |
work_keys_str_mv | AT lindongbo slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato AT zhuxiaoen slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato AT qibinglin slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato AT gaozhong slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato AT tianpeng slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato AT liziwei slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato AT linzeteng slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato AT zhangyongxia slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato AT huangtengbo slmir164aregulatesfruitripeningandqualitybycontrollingslnam2andslnam3intomato |