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SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato

MiRNAs are important posttranscriptional regulators of plant development. Many miRNAs, such as the conserved miR164 species, are encoded by families of MIRNA genes, but the specific roles of individual MIRNA genes are largely undefined. Here, we characterize the functions and regulatory mechanisms o...

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Autores principales: Lin, Dongbo, Zhu, Xiaoen, Qi, Binglin, Gao, Zhong, Tian, Peng, Li, Ziwei, Lin, Zeteng, Zhang, Yongxia, Huang, Tengbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342619/
https://www.ncbi.nlm.nih.gov/pubmed/35403821
http://dx.doi.org/10.1111/pbi.13824
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author Lin, Dongbo
Zhu, Xiaoen
Qi, Binglin
Gao, Zhong
Tian, Peng
Li, Ziwei
Lin, Zeteng
Zhang, Yongxia
Huang, Tengbo
author_facet Lin, Dongbo
Zhu, Xiaoen
Qi, Binglin
Gao, Zhong
Tian, Peng
Li, Ziwei
Lin, Zeteng
Zhang, Yongxia
Huang, Tengbo
author_sort Lin, Dongbo
collection PubMed
description MiRNAs are important posttranscriptional regulators of plant development. Many miRNAs, such as the conserved miR164 species, are encoded by families of MIRNA genes, but the specific roles of individual MIRNA genes are largely undefined. Here, we characterize the functions and regulatory mechanisms of SlMIR164A, one of the primary genes of Sly‐miR164, in tomato. We show that SlMIR164A is preferentially expressed at late stages of fruit development and plays a vital role in controlling fruit ripening and quality. Loss of function of SlMIR164A by CRISPR/Cas9‐mediated mutagenesis results in accelerated fruit ripening and enhanced chloroplast development, which leads to altered sugar and organic acid contents and affects the nutritional quality of fruits. We also show that SlMIR164A modulates fruit ripening and quality through specific target genes, SlNAM2 and SlNAM3, which control key regulators of chloroplast function and fruit ripening processes. MIR164 genes have been shown to play conserved roles in regulating organ ageing, such as leaf senescence and fruit ripening, in a variety of plants, but whether and how their family members in tomato exert the same function remain to be elucidated. Our results reveal a previously undiscovered role of SlMIR164A in ripening control, which will further our understanding of the actions of MIR164 family, as well as the mechanisms of fruit ripening and quality control in tomato. Moreover, as loss of SlMIR164A exhibits minor impacts on organ morphology, our results can be leveraged in tomato breeding for specific manipulation of fruit ripening and quality to facilitate tomato improvement in agriculture.
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spelling pubmed-93426192022-08-03 SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato Lin, Dongbo Zhu, Xiaoen Qi, Binglin Gao, Zhong Tian, Peng Li, Ziwei Lin, Zeteng Zhang, Yongxia Huang, Tengbo Plant Biotechnol J Research Articles MiRNAs are important posttranscriptional regulators of plant development. Many miRNAs, such as the conserved miR164 species, are encoded by families of MIRNA genes, but the specific roles of individual MIRNA genes are largely undefined. Here, we characterize the functions and regulatory mechanisms of SlMIR164A, one of the primary genes of Sly‐miR164, in tomato. We show that SlMIR164A is preferentially expressed at late stages of fruit development and plays a vital role in controlling fruit ripening and quality. Loss of function of SlMIR164A by CRISPR/Cas9‐mediated mutagenesis results in accelerated fruit ripening and enhanced chloroplast development, which leads to altered sugar and organic acid contents and affects the nutritional quality of fruits. We also show that SlMIR164A modulates fruit ripening and quality through specific target genes, SlNAM2 and SlNAM3, which control key regulators of chloroplast function and fruit ripening processes. MIR164 genes have been shown to play conserved roles in regulating organ ageing, such as leaf senescence and fruit ripening, in a variety of plants, but whether and how their family members in tomato exert the same function remain to be elucidated. Our results reveal a previously undiscovered role of SlMIR164A in ripening control, which will further our understanding of the actions of MIR164 family, as well as the mechanisms of fruit ripening and quality control in tomato. Moreover, as loss of SlMIR164A exhibits minor impacts on organ morphology, our results can be leveraged in tomato breeding for specific manipulation of fruit ripening and quality to facilitate tomato improvement in agriculture. John Wiley and Sons Inc. 2022-04-28 2022-08 /pmc/articles/PMC9342619/ /pubmed/35403821 http://dx.doi.org/10.1111/pbi.13824 Text en © 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Lin, Dongbo
Zhu, Xiaoen
Qi, Binglin
Gao, Zhong
Tian, Peng
Li, Ziwei
Lin, Zeteng
Zhang, Yongxia
Huang, Tengbo
SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato
title SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato
title_full SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato
title_fullStr SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato
title_full_unstemmed SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato
title_short SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato
title_sort slmir164a regulates fruit ripening and quality by controlling slnam2 and slnam3 in tomato
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342619/
https://www.ncbi.nlm.nih.gov/pubmed/35403821
http://dx.doi.org/10.1111/pbi.13824
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