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PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers

Stretching individual living cells with light is a standard method to assess their mechanical properties. Yet, heat introduced by the laser light of optical stretchers may unwittingly change the mechanical properties of cells therein. To estimate the temperature induced by an optical trap, we introd...

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Autores principales: Hauck, Nicolas, Beck, Timon, Cojoc, Gheorghe, Schlüßler, Raimund, Ahmed, Saeed, Raguzin, Ivan, Mayer, Martin, Schubert, Jonas, Müller, Paul, Guck, Jochen, Thiele, Julian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342673/
https://www.ncbi.nlm.nih.gov/pubmed/35979502
http://dx.doi.org/10.1039/d2ma00296e
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author Hauck, Nicolas
Beck, Timon
Cojoc, Gheorghe
Schlüßler, Raimund
Ahmed, Saeed
Raguzin, Ivan
Mayer, Martin
Schubert, Jonas
Müller, Paul
Guck, Jochen
Thiele, Julian
author_facet Hauck, Nicolas
Beck, Timon
Cojoc, Gheorghe
Schlüßler, Raimund
Ahmed, Saeed
Raguzin, Ivan
Mayer, Martin
Schubert, Jonas
Müller, Paul
Guck, Jochen
Thiele, Julian
author_sort Hauck, Nicolas
collection PubMed
description Stretching individual living cells with light is a standard method to assess their mechanical properties. Yet, heat introduced by the laser light of optical stretchers may unwittingly change the mechanical properties of cells therein. To estimate the temperature induced by an optical trap, we introduce cell-sized, elastic poly(N-isopropylacrylamide) (PNIPAAm) microgels that relate temperature changes to hydrogel swelling. For their usage as a standardized calibration tool, we analyze the effect of free-radical chain-growth gelation (FCG) and polymer-analogous photogelation (PAG) on hydrogel network heterogeneity, micromechanics, and temperature response by Brillouin microscopy and optical diffraction tomography. Using a combination of tailor-made PNIPAAm macromers, PAG, and microfluidic processing, we obtain microgels with homogeneous network architecture. With that, we expand the capability of standardized microgels in calibrating and validating cell mechanics analysis, not only considering cell and microgel elasticity but also providing stimuli-responsiveness to consider dynamic changes that cells may undergo during characterization.
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spelling pubmed-93426732022-08-15 PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers Hauck, Nicolas Beck, Timon Cojoc, Gheorghe Schlüßler, Raimund Ahmed, Saeed Raguzin, Ivan Mayer, Martin Schubert, Jonas Müller, Paul Guck, Jochen Thiele, Julian Mater Adv Chemistry Stretching individual living cells with light is a standard method to assess their mechanical properties. Yet, heat introduced by the laser light of optical stretchers may unwittingly change the mechanical properties of cells therein. To estimate the temperature induced by an optical trap, we introduce cell-sized, elastic poly(N-isopropylacrylamide) (PNIPAAm) microgels that relate temperature changes to hydrogel swelling. For their usage as a standardized calibration tool, we analyze the effect of free-radical chain-growth gelation (FCG) and polymer-analogous photogelation (PAG) on hydrogel network heterogeneity, micromechanics, and temperature response by Brillouin microscopy and optical diffraction tomography. Using a combination of tailor-made PNIPAAm macromers, PAG, and microfluidic processing, we obtain microgels with homogeneous network architecture. With that, we expand the capability of standardized microgels in calibrating and validating cell mechanics analysis, not only considering cell and microgel elasticity but also providing stimuli-responsiveness to consider dynamic changes that cells may undergo during characterization. RSC 2022-07-05 /pmc/articles/PMC9342673/ /pubmed/35979502 http://dx.doi.org/10.1039/d2ma00296e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Hauck, Nicolas
Beck, Timon
Cojoc, Gheorghe
Schlüßler, Raimund
Ahmed, Saeed
Raguzin, Ivan
Mayer, Martin
Schubert, Jonas
Müller, Paul
Guck, Jochen
Thiele, Julian
PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers
title PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers
title_full PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers
title_fullStr PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers
title_full_unstemmed PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers
title_short PNIPAAm microgels with defined network architecture as temperature sensors in optical stretchers
title_sort pnipaam microgels with defined network architecture as temperature sensors in optical stretchers
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342673/
https://www.ncbi.nlm.nih.gov/pubmed/35979502
http://dx.doi.org/10.1039/d2ma00296e
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