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The bending rigidity of the red blood cell cytoplasmic membrane
An important mechanical property of cells is the membrane bending modulus, κ. In the case of red blood cells (RBCs) there is a composite membrane consisting of a cytoplasmic membrane and an underlying spectrin network. Literature values of κ are puzzling, as they are reported over a wide range, from...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342732/ https://www.ncbi.nlm.nih.gov/pubmed/35913930 http://dx.doi.org/10.1371/journal.pone.0269619 |
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author | Himbert, Sebastian D’Alessandro, Angelo Qadri, Syed M. Majcher, Michael J. Hoare, Todd Sheffield, William P. Nagao, Michihiro Nagle, John F. Rheinstädter, Maikel C. |
author_facet | Himbert, Sebastian D’Alessandro, Angelo Qadri, Syed M. Majcher, Michael J. Hoare, Todd Sheffield, William P. Nagao, Michihiro Nagle, John F. Rheinstädter, Maikel C. |
author_sort | Himbert, Sebastian |
collection | PubMed |
description | An important mechanical property of cells is the membrane bending modulus, κ. In the case of red blood cells (RBCs) there is a composite membrane consisting of a cytoplasmic membrane and an underlying spectrin network. Literature values of κ are puzzling, as they are reported over a wide range, from 5 k(B)T to 230 k(B)T. To disentangle the contribution of the cytoplasmic membrane from the spectrin network, we investigated the bending of red blood cell cytoplasmic membranes (RBC(cm)) in the absence of spectrin and adenosine triphosphate (ATP). We used a combination of X-ray diffuse scattering (XDS), neutron spin-echo (NSE) spectrometry and Molecular Dynamics (MD) simulations. Our results indicate values of κ of order 4 k(B)T to 6 k(B)T, relatively small compared to literature values for most single component lipid bilayers. We suggest two ways this relative softness might confer biological advantage. |
format | Online Article Text |
id | pubmed-9342732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-93427322022-08-02 The bending rigidity of the red blood cell cytoplasmic membrane Himbert, Sebastian D’Alessandro, Angelo Qadri, Syed M. Majcher, Michael J. Hoare, Todd Sheffield, William P. Nagao, Michihiro Nagle, John F. Rheinstädter, Maikel C. PLoS One Research Article An important mechanical property of cells is the membrane bending modulus, κ. In the case of red blood cells (RBCs) there is a composite membrane consisting of a cytoplasmic membrane and an underlying spectrin network. Literature values of κ are puzzling, as they are reported over a wide range, from 5 k(B)T to 230 k(B)T. To disentangle the contribution of the cytoplasmic membrane from the spectrin network, we investigated the bending of red blood cell cytoplasmic membranes (RBC(cm)) in the absence of spectrin and adenosine triphosphate (ATP). We used a combination of X-ray diffuse scattering (XDS), neutron spin-echo (NSE) spectrometry and Molecular Dynamics (MD) simulations. Our results indicate values of κ of order 4 k(B)T to 6 k(B)T, relatively small compared to literature values for most single component lipid bilayers. We suggest two ways this relative softness might confer biological advantage. Public Library of Science 2022-08-01 /pmc/articles/PMC9342732/ /pubmed/35913930 http://dx.doi.org/10.1371/journal.pone.0269619 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Himbert, Sebastian D’Alessandro, Angelo Qadri, Syed M. Majcher, Michael J. Hoare, Todd Sheffield, William P. Nagao, Michihiro Nagle, John F. Rheinstädter, Maikel C. The bending rigidity of the red blood cell cytoplasmic membrane |
title | The bending rigidity of the red blood cell cytoplasmic membrane |
title_full | The bending rigidity of the red blood cell cytoplasmic membrane |
title_fullStr | The bending rigidity of the red blood cell cytoplasmic membrane |
title_full_unstemmed | The bending rigidity of the red blood cell cytoplasmic membrane |
title_short | The bending rigidity of the red blood cell cytoplasmic membrane |
title_sort | bending rigidity of the red blood cell cytoplasmic membrane |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342732/ https://www.ncbi.nlm.nih.gov/pubmed/35913930 http://dx.doi.org/10.1371/journal.pone.0269619 |
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