Cargando…
Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium
OBJECTIVE: To investigate the plasma proteomic profiling in identifying biomarkers related to return to sport (RTS) following a sport-related concussion (SRC). METHODS: This multicenter, prospective, case-control study was part of a larger cohort study conducted by the NCAA-DoD Concussion Assessment...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343581/ https://www.ncbi.nlm.nih.gov/pubmed/35928129 http://dx.doi.org/10.3389/fneur.2022.901238 |
_version_ | 1784761021471129600 |
---|---|
author | Vorn, Rany Mithani, Sara Devoto, Christina Meier, Timothy B. Lai, Chen Yun, Sijung Broglio, Steven P. McAllister, Thomas W. Giza, Christopher C. Kim, Hyung-Suk Huber, Daniel Harezlak, Jaroslaw Cameron, Kenneth L. McGinty, Gerald Jackson, Jonathan Guskiewicz, Kevin M. Mihalik, Jason P. Brooks, Alison Duma, Stefan Rowson, Steven Nelson, Lindsay D. Pasquina, Paul McCrea, Michael A. Gill, Jessica M. |
author_facet | Vorn, Rany Mithani, Sara Devoto, Christina Meier, Timothy B. Lai, Chen Yun, Sijung Broglio, Steven P. McAllister, Thomas W. Giza, Christopher C. Kim, Hyung-Suk Huber, Daniel Harezlak, Jaroslaw Cameron, Kenneth L. McGinty, Gerald Jackson, Jonathan Guskiewicz, Kevin M. Mihalik, Jason P. Brooks, Alison Duma, Stefan Rowson, Steven Nelson, Lindsay D. Pasquina, Paul McCrea, Michael A. Gill, Jessica M. |
author_sort | Vorn, Rany |
collection | PubMed |
description | OBJECTIVE: To investigate the plasma proteomic profiling in identifying biomarkers related to return to sport (RTS) following a sport-related concussion (SRC). METHODS: This multicenter, prospective, case-control study was part of a larger cohort study conducted by the NCAA-DoD Concussion Assessment, Research, and Education (CARE) Consortium, athletes (n = 140) with blood collected within 48 h of injury and reported day to asymptomatic were included in this study, divided into two groups: (1) recovery <14-days (n = 99) and (2) recovery ≥14-days (n = 41). We applied a highly multiplexed proteomic technique that uses DNA aptamers assay to target 1,305 proteins in plasma samples from concussed athletes with <14-days and ≥14-days. RESULTS: We identified 87 plasma proteins significantly dysregulated (32 upregulated and 55 downregulated) in concussed athletes with recovery ≥14-days relative to recovery <14-days groups. The significantly dysregulated proteins were uploaded to Ingenuity Pathway Analysis (IPA) software for analysis. Pathway analysis showed that significantly dysregulated proteins were associated with STAT3 pathway, regulation of the epithelial mesenchymal transition by growth factors pathway, and acute phase response signaling. CONCLUSION: Our data showed the feasibility of large-scale plasma proteomic profiling in concussed athletes with a <14-days and ≥ 14-days recovery. These findings provide a possible understanding of the pathophysiological mechanism in neurobiological recovery. Further study is required to determine whether these proteins can aid clinicians in RTS decisions. |
format | Online Article Text |
id | pubmed-9343581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93435812022-08-03 Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium Vorn, Rany Mithani, Sara Devoto, Christina Meier, Timothy B. Lai, Chen Yun, Sijung Broglio, Steven P. McAllister, Thomas W. Giza, Christopher C. Kim, Hyung-Suk Huber, Daniel Harezlak, Jaroslaw Cameron, Kenneth L. McGinty, Gerald Jackson, Jonathan Guskiewicz, Kevin M. Mihalik, Jason P. Brooks, Alison Duma, Stefan Rowson, Steven Nelson, Lindsay D. Pasquina, Paul McCrea, Michael A. Gill, Jessica M. Front Neurol Neurology OBJECTIVE: To investigate the plasma proteomic profiling in identifying biomarkers related to return to sport (RTS) following a sport-related concussion (SRC). METHODS: This multicenter, prospective, case-control study was part of a larger cohort study conducted by the NCAA-DoD Concussion Assessment, Research, and Education (CARE) Consortium, athletes (n = 140) with blood collected within 48 h of injury and reported day to asymptomatic were included in this study, divided into two groups: (1) recovery <14-days (n = 99) and (2) recovery ≥14-days (n = 41). We applied a highly multiplexed proteomic technique that uses DNA aptamers assay to target 1,305 proteins in plasma samples from concussed athletes with <14-days and ≥14-days. RESULTS: We identified 87 plasma proteins significantly dysregulated (32 upregulated and 55 downregulated) in concussed athletes with recovery ≥14-days relative to recovery <14-days groups. The significantly dysregulated proteins were uploaded to Ingenuity Pathway Analysis (IPA) software for analysis. Pathway analysis showed that significantly dysregulated proteins were associated with STAT3 pathway, regulation of the epithelial mesenchymal transition by growth factors pathway, and acute phase response signaling. CONCLUSION: Our data showed the feasibility of large-scale plasma proteomic profiling in concussed athletes with a <14-days and ≥ 14-days recovery. These findings provide a possible understanding of the pathophysiological mechanism in neurobiological recovery. Further study is required to determine whether these proteins can aid clinicians in RTS decisions. Frontiers Media S.A. 2022-07-19 /pmc/articles/PMC9343581/ /pubmed/35928129 http://dx.doi.org/10.3389/fneur.2022.901238 Text en Copyright © 2022 Vorn, Mithani, Devoto, Meier, Lai, Yun, Broglio, McAllister, Giza, Kim, Huber, Harezlak, Cameron, McGinty, Jackson, Guskiewicz, Mihalik, Brooks, Duma, Rowson, Nelson, Pasquina, McCrea and Gill. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neurology Vorn, Rany Mithani, Sara Devoto, Christina Meier, Timothy B. Lai, Chen Yun, Sijung Broglio, Steven P. McAllister, Thomas W. Giza, Christopher C. Kim, Hyung-Suk Huber, Daniel Harezlak, Jaroslaw Cameron, Kenneth L. McGinty, Gerald Jackson, Jonathan Guskiewicz, Kevin M. Mihalik, Jason P. Brooks, Alison Duma, Stefan Rowson, Steven Nelson, Lindsay D. Pasquina, Paul McCrea, Michael A. Gill, Jessica M. Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium |
title | Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium |
title_full | Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium |
title_fullStr | Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium |
title_full_unstemmed | Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium |
title_short | Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium |
title_sort | proteomic profiling of plasma biomarkers associated with return to sport following concussion: findings from the ncaa and department of defense care consortium |
topic | Neurology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343581/ https://www.ncbi.nlm.nih.gov/pubmed/35928129 http://dx.doi.org/10.3389/fneur.2022.901238 |
work_keys_str_mv | AT vornrany proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT mithanisara proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT devotochristina proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT meiertimothyb proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT laichen proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT yunsijung proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT brogliostevenp proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT mcallisterthomasw proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT gizachristopherc proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT kimhyungsuk proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT huberdaniel proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT harezlakjaroslaw proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT cameronkennethl proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT mcgintygerald proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT jacksonjonathan proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT guskiewiczkevinm proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT mihalikjasonp proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT brooksalison proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT dumastefan proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT rowsonsteven proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT nelsonlindsayd proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT pasquinapaul proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT mccreamichaela proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium AT gilljessicam proteomicprofilingofplasmabiomarkersassociatedwithreturntosportfollowingconcussionfindingsfromthencaaanddepartmentofdefensecareconsortium |