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Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium

OBJECTIVE: To investigate the plasma proteomic profiling in identifying biomarkers related to return to sport (RTS) following a sport-related concussion (SRC). METHODS: This multicenter, prospective, case-control study was part of a larger cohort study conducted by the NCAA-DoD Concussion Assessment...

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Autores principales: Vorn, Rany, Mithani, Sara, Devoto, Christina, Meier, Timothy B., Lai, Chen, Yun, Sijung, Broglio, Steven P., McAllister, Thomas W., Giza, Christopher C., Kim, Hyung-Suk, Huber, Daniel, Harezlak, Jaroslaw, Cameron, Kenneth L., McGinty, Gerald, Jackson, Jonathan, Guskiewicz, Kevin M., Mihalik, Jason P., Brooks, Alison, Duma, Stefan, Rowson, Steven, Nelson, Lindsay D., Pasquina, Paul, McCrea, Michael A., Gill, Jessica M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343581/
https://www.ncbi.nlm.nih.gov/pubmed/35928129
http://dx.doi.org/10.3389/fneur.2022.901238
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author Vorn, Rany
Mithani, Sara
Devoto, Christina
Meier, Timothy B.
Lai, Chen
Yun, Sijung
Broglio, Steven P.
McAllister, Thomas W.
Giza, Christopher C.
Kim, Hyung-Suk
Huber, Daniel
Harezlak, Jaroslaw
Cameron, Kenneth L.
McGinty, Gerald
Jackson, Jonathan
Guskiewicz, Kevin M.
Mihalik, Jason P.
Brooks, Alison
Duma, Stefan
Rowson, Steven
Nelson, Lindsay D.
Pasquina, Paul
McCrea, Michael A.
Gill, Jessica M.
author_facet Vorn, Rany
Mithani, Sara
Devoto, Christina
Meier, Timothy B.
Lai, Chen
Yun, Sijung
Broglio, Steven P.
McAllister, Thomas W.
Giza, Christopher C.
Kim, Hyung-Suk
Huber, Daniel
Harezlak, Jaroslaw
Cameron, Kenneth L.
McGinty, Gerald
Jackson, Jonathan
Guskiewicz, Kevin M.
Mihalik, Jason P.
Brooks, Alison
Duma, Stefan
Rowson, Steven
Nelson, Lindsay D.
Pasquina, Paul
McCrea, Michael A.
Gill, Jessica M.
author_sort Vorn, Rany
collection PubMed
description OBJECTIVE: To investigate the plasma proteomic profiling in identifying biomarkers related to return to sport (RTS) following a sport-related concussion (SRC). METHODS: This multicenter, prospective, case-control study was part of a larger cohort study conducted by the NCAA-DoD Concussion Assessment, Research, and Education (CARE) Consortium, athletes (n = 140) with blood collected within 48 h of injury and reported day to asymptomatic were included in this study, divided into two groups: (1) recovery <14-days (n = 99) and (2) recovery ≥14-days (n = 41). We applied a highly multiplexed proteomic technique that uses DNA aptamers assay to target 1,305 proteins in plasma samples from concussed athletes with <14-days and ≥14-days. RESULTS: We identified 87 plasma proteins significantly dysregulated (32 upregulated and 55 downregulated) in concussed athletes with recovery ≥14-days relative to recovery <14-days groups. The significantly dysregulated proteins were uploaded to Ingenuity Pathway Analysis (IPA) software for analysis. Pathway analysis showed that significantly dysregulated proteins were associated with STAT3 pathway, regulation of the epithelial mesenchymal transition by growth factors pathway, and acute phase response signaling. CONCLUSION: Our data showed the feasibility of large-scale plasma proteomic profiling in concussed athletes with a <14-days and ≥ 14-days recovery. These findings provide a possible understanding of the pathophysiological mechanism in neurobiological recovery. Further study is required to determine whether these proteins can aid clinicians in RTS decisions.
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spelling pubmed-93435812022-08-03 Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium Vorn, Rany Mithani, Sara Devoto, Christina Meier, Timothy B. Lai, Chen Yun, Sijung Broglio, Steven P. McAllister, Thomas W. Giza, Christopher C. Kim, Hyung-Suk Huber, Daniel Harezlak, Jaroslaw Cameron, Kenneth L. McGinty, Gerald Jackson, Jonathan Guskiewicz, Kevin M. Mihalik, Jason P. Brooks, Alison Duma, Stefan Rowson, Steven Nelson, Lindsay D. Pasquina, Paul McCrea, Michael A. Gill, Jessica M. Front Neurol Neurology OBJECTIVE: To investigate the plasma proteomic profiling in identifying biomarkers related to return to sport (RTS) following a sport-related concussion (SRC). METHODS: This multicenter, prospective, case-control study was part of a larger cohort study conducted by the NCAA-DoD Concussion Assessment, Research, and Education (CARE) Consortium, athletes (n = 140) with blood collected within 48 h of injury and reported day to asymptomatic were included in this study, divided into two groups: (1) recovery <14-days (n = 99) and (2) recovery ≥14-days (n = 41). We applied a highly multiplexed proteomic technique that uses DNA aptamers assay to target 1,305 proteins in plasma samples from concussed athletes with <14-days and ≥14-days. RESULTS: We identified 87 plasma proteins significantly dysregulated (32 upregulated and 55 downregulated) in concussed athletes with recovery ≥14-days relative to recovery <14-days groups. The significantly dysregulated proteins were uploaded to Ingenuity Pathway Analysis (IPA) software for analysis. Pathway analysis showed that significantly dysregulated proteins were associated with STAT3 pathway, regulation of the epithelial mesenchymal transition by growth factors pathway, and acute phase response signaling. CONCLUSION: Our data showed the feasibility of large-scale plasma proteomic profiling in concussed athletes with a <14-days and ≥ 14-days recovery. These findings provide a possible understanding of the pathophysiological mechanism in neurobiological recovery. Further study is required to determine whether these proteins can aid clinicians in RTS decisions. Frontiers Media S.A. 2022-07-19 /pmc/articles/PMC9343581/ /pubmed/35928129 http://dx.doi.org/10.3389/fneur.2022.901238 Text en Copyright © 2022 Vorn, Mithani, Devoto, Meier, Lai, Yun, Broglio, McAllister, Giza, Kim, Huber, Harezlak, Cameron, McGinty, Jackson, Guskiewicz, Mihalik, Brooks, Duma, Rowson, Nelson, Pasquina, McCrea and Gill. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neurology
Vorn, Rany
Mithani, Sara
Devoto, Christina
Meier, Timothy B.
Lai, Chen
Yun, Sijung
Broglio, Steven P.
McAllister, Thomas W.
Giza, Christopher C.
Kim, Hyung-Suk
Huber, Daniel
Harezlak, Jaroslaw
Cameron, Kenneth L.
McGinty, Gerald
Jackson, Jonathan
Guskiewicz, Kevin M.
Mihalik, Jason P.
Brooks, Alison
Duma, Stefan
Rowson, Steven
Nelson, Lindsay D.
Pasquina, Paul
McCrea, Michael A.
Gill, Jessica M.
Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium
title Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium
title_full Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium
title_fullStr Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium
title_full_unstemmed Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium
title_short Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium
title_sort proteomic profiling of plasma biomarkers associated with return to sport following concussion: findings from the ncaa and department of defense care consortium
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343581/
https://www.ncbi.nlm.nih.gov/pubmed/35928129
http://dx.doi.org/10.3389/fneur.2022.901238
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