Cargando…

Invadopodia enable cooperative invasion and metastasis of breast cancer cells

Invasive and non-invasive cancer cells can invade together during cooperative invasion. However, the events leading to it, role of the epithelial-mesenchymal transition and the consequences this may have on metastasis are unknown. In this study, we demonstrate that the isogenic 4T1 and 67NR breast c...

Descripción completa

Detalles Bibliográficos
Autores principales: Perrin, Louisiane, Belova, Elizaveta, Bayarmagnai, Battuya, Tüzel, Erkan, Gligorijevic, Bojana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343607/
https://www.ncbi.nlm.nih.gov/pubmed/35915226
http://dx.doi.org/10.1038/s42003-022-03642-z
_version_ 1784761027133440000
author Perrin, Louisiane
Belova, Elizaveta
Bayarmagnai, Battuya
Tüzel, Erkan
Gligorijevic, Bojana
author_facet Perrin, Louisiane
Belova, Elizaveta
Bayarmagnai, Battuya
Tüzel, Erkan
Gligorijevic, Bojana
author_sort Perrin, Louisiane
collection PubMed
description Invasive and non-invasive cancer cells can invade together during cooperative invasion. However, the events leading to it, role of the epithelial-mesenchymal transition and the consequences this may have on metastasis are unknown. In this study, we demonstrate that the isogenic 4T1 and 67NR breast cancer cells sort from each other in 3D spheroids, followed by cooperative invasion. By time-lapse microscopy, we show that the invasive 4T1 cells move more persistently compared to non-invasive 67NR, sorting and accumulating at the spheroid-matrix interface, a process dependent on cell-matrix adhesions and independent from E-cadherin cell-cell adhesions. Elimination of invadopodia in 4T1 cells blocks invasion, demonstrating that invadopodia requirement is limited to leader cells. Importantly, we demonstrate that cells with and without invadopodia can also engage in cooperative metastasis in preclinical mouse models. Altogether, our results suggest that a small number of cells with invadopodia can drive the metastasis of heterogeneous cell clusters.
format Online
Article
Text
id pubmed-9343607
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-93436072022-08-03 Invadopodia enable cooperative invasion and metastasis of breast cancer cells Perrin, Louisiane Belova, Elizaveta Bayarmagnai, Battuya Tüzel, Erkan Gligorijevic, Bojana Commun Biol Article Invasive and non-invasive cancer cells can invade together during cooperative invasion. However, the events leading to it, role of the epithelial-mesenchymal transition and the consequences this may have on metastasis are unknown. In this study, we demonstrate that the isogenic 4T1 and 67NR breast cancer cells sort from each other in 3D spheroids, followed by cooperative invasion. By time-lapse microscopy, we show that the invasive 4T1 cells move more persistently compared to non-invasive 67NR, sorting and accumulating at the spheroid-matrix interface, a process dependent on cell-matrix adhesions and independent from E-cadherin cell-cell adhesions. Elimination of invadopodia in 4T1 cells blocks invasion, demonstrating that invadopodia requirement is limited to leader cells. Importantly, we demonstrate that cells with and without invadopodia can also engage in cooperative metastasis in preclinical mouse models. Altogether, our results suggest that a small number of cells with invadopodia can drive the metastasis of heterogeneous cell clusters. Nature Publishing Group UK 2022-08-01 /pmc/articles/PMC9343607/ /pubmed/35915226 http://dx.doi.org/10.1038/s42003-022-03642-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Perrin, Louisiane
Belova, Elizaveta
Bayarmagnai, Battuya
Tüzel, Erkan
Gligorijevic, Bojana
Invadopodia enable cooperative invasion and metastasis of breast cancer cells
title Invadopodia enable cooperative invasion and metastasis of breast cancer cells
title_full Invadopodia enable cooperative invasion and metastasis of breast cancer cells
title_fullStr Invadopodia enable cooperative invasion and metastasis of breast cancer cells
title_full_unstemmed Invadopodia enable cooperative invasion and metastasis of breast cancer cells
title_short Invadopodia enable cooperative invasion and metastasis of breast cancer cells
title_sort invadopodia enable cooperative invasion and metastasis of breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343607/
https://www.ncbi.nlm.nih.gov/pubmed/35915226
http://dx.doi.org/10.1038/s42003-022-03642-z
work_keys_str_mv AT perrinlouisiane invadopodiaenablecooperativeinvasionandmetastasisofbreastcancercells
AT belovaelizaveta invadopodiaenablecooperativeinvasionandmetastasisofbreastcancercells
AT bayarmagnaibattuya invadopodiaenablecooperativeinvasionandmetastasisofbreastcancercells
AT tuzelerkan invadopodiaenablecooperativeinvasionandmetastasisofbreastcancercells
AT gligorijevicbojana invadopodiaenablecooperativeinvasionandmetastasisofbreastcancercells