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Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration
Sericin, a waste product of the silk textile industry, has favorable physicochemical and biological properties. In this study, we extracted a low molecular weight (MW) sericin (LMW-sericin; below 10 kDa) by a performing high-temperature and high-pressure method and confirmed the MW using matrix-assi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343859/ https://www.ncbi.nlm.nih.gov/pubmed/35928949 http://dx.doi.org/10.3389/fbioe.2022.925197 |
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author | Cherng, Juin-Hong Chang, Shu-Jen Chiu, Yaw-Kwan Chiu, Yu-Hsiang Fang, Tong-Jing Chen, Hsiang-Cheng |
author_facet | Cherng, Juin-Hong Chang, Shu-Jen Chiu, Yaw-Kwan Chiu, Yu-Hsiang Fang, Tong-Jing Chen, Hsiang-Cheng |
author_sort | Cherng, Juin-Hong |
collection | PubMed |
description | Sericin, a waste product of the silk textile industry, has favorable physicochemical and biological properties. In this study, we extracted a low molecular weight (MW) sericin (LMW-sericin; below 10 kDa) by a performing high-temperature and high-pressure method and confirmed the MW using matrix-assisted laser desorption ionization-time of flight and liquid chromatography–mass spectrometry. Furthermore, we determined its biological effects on macrophages and human adipose stem cells (hASCs) as cell models to investigate the biocompatibility, immunomodulation behavior, and potential signaling pathway-related wound healing via analyses of gene expression of focal adhesion and human cytokines and chemokines using quantitative real-time polymerase chain reaction and cytokine assay. LMW-sericin showed good biocompatibility both in macrophages and hASCs. Macrophages cultured with 0.1 mg/ml LMW-sericin displayed an improved inflammatory response shown by the upregulation of CXCL9, IL12A, BMP7, and IL10, which developed Th1 and Th2 balance. LMW-sericin also improved the differentiation of macrophages toward the M2 phenotype by significantly enhancing the expression of Arg-1, which is conducive to the repair of the inflammatory environment. Moreover, the gene expression of hASCs showed that LMW-sericin promoted the secretion of beneficial adhesion molecules that potentially activate the gene transcription of differentiation and migration in hASCs, as well as significantly enhanced the levels of PKCβ1, RhoA, and RasGFR1 as fruitful molecules in wound healing. These findings provide insights into LMW-sericin application as a potential biomaterial for wound management. |
format | Online Article Text |
id | pubmed-9343859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93438592022-08-03 Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration Cherng, Juin-Hong Chang, Shu-Jen Chiu, Yaw-Kwan Chiu, Yu-Hsiang Fang, Tong-Jing Chen, Hsiang-Cheng Front Bioeng Biotechnol Bioengineering and Biotechnology Sericin, a waste product of the silk textile industry, has favorable physicochemical and biological properties. In this study, we extracted a low molecular weight (MW) sericin (LMW-sericin; below 10 kDa) by a performing high-temperature and high-pressure method and confirmed the MW using matrix-assisted laser desorption ionization-time of flight and liquid chromatography–mass spectrometry. Furthermore, we determined its biological effects on macrophages and human adipose stem cells (hASCs) as cell models to investigate the biocompatibility, immunomodulation behavior, and potential signaling pathway-related wound healing via analyses of gene expression of focal adhesion and human cytokines and chemokines using quantitative real-time polymerase chain reaction and cytokine assay. LMW-sericin showed good biocompatibility both in macrophages and hASCs. Macrophages cultured with 0.1 mg/ml LMW-sericin displayed an improved inflammatory response shown by the upregulation of CXCL9, IL12A, BMP7, and IL10, which developed Th1 and Th2 balance. LMW-sericin also improved the differentiation of macrophages toward the M2 phenotype by significantly enhancing the expression of Arg-1, which is conducive to the repair of the inflammatory environment. Moreover, the gene expression of hASCs showed that LMW-sericin promoted the secretion of beneficial adhesion molecules that potentially activate the gene transcription of differentiation and migration in hASCs, as well as significantly enhanced the levels of PKCβ1, RhoA, and RasGFR1 as fruitful molecules in wound healing. These findings provide insights into LMW-sericin application as a potential biomaterial for wound management. Frontiers Media S.A. 2022-07-19 /pmc/articles/PMC9343859/ /pubmed/35928949 http://dx.doi.org/10.3389/fbioe.2022.925197 Text en Copyright © 2022 Cherng, Chang, Chiu, Chiu, Fang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Cherng, Juin-Hong Chang, Shu-Jen Chiu, Yaw-Kwan Chiu, Yu-Hsiang Fang, Tong-Jing Chen, Hsiang-Cheng Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration |
title | Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration |
title_full | Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration |
title_fullStr | Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration |
title_full_unstemmed | Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration |
title_short | Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration |
title_sort | low molecular weight sericin enhances the in vitro of immunological modulation and cell migration |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343859/ https://www.ncbi.nlm.nih.gov/pubmed/35928949 http://dx.doi.org/10.3389/fbioe.2022.925197 |
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