Cargando…

Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration

Sericin, a waste product of the silk textile industry, has favorable physicochemical and biological properties. In this study, we extracted a low molecular weight (MW) sericin (LMW-sericin; below 10 kDa) by a performing high-temperature and high-pressure method and confirmed the MW using matrix-assi...

Descripción completa

Detalles Bibliográficos
Autores principales: Cherng, Juin-Hong, Chang, Shu-Jen, Chiu, Yaw-Kwan, Chiu, Yu-Hsiang, Fang, Tong-Jing, Chen, Hsiang-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343859/
https://www.ncbi.nlm.nih.gov/pubmed/35928949
http://dx.doi.org/10.3389/fbioe.2022.925197
_version_ 1784761085777149952
author Cherng, Juin-Hong
Chang, Shu-Jen
Chiu, Yaw-Kwan
Chiu, Yu-Hsiang
Fang, Tong-Jing
Chen, Hsiang-Cheng
author_facet Cherng, Juin-Hong
Chang, Shu-Jen
Chiu, Yaw-Kwan
Chiu, Yu-Hsiang
Fang, Tong-Jing
Chen, Hsiang-Cheng
author_sort Cherng, Juin-Hong
collection PubMed
description Sericin, a waste product of the silk textile industry, has favorable physicochemical and biological properties. In this study, we extracted a low molecular weight (MW) sericin (LMW-sericin; below 10 kDa) by a performing high-temperature and high-pressure method and confirmed the MW using matrix-assisted laser desorption ionization-time of flight and liquid chromatography–mass spectrometry. Furthermore, we determined its biological effects on macrophages and human adipose stem cells (hASCs) as cell models to investigate the biocompatibility, immunomodulation behavior, and potential signaling pathway-related wound healing via analyses of gene expression of focal adhesion and human cytokines and chemokines using quantitative real-time polymerase chain reaction and cytokine assay. LMW-sericin showed good biocompatibility both in macrophages and hASCs. Macrophages cultured with 0.1 mg/ml LMW-sericin displayed an improved inflammatory response shown by the upregulation of CXCL9, IL12A, BMP7, and IL10, which developed Th1 and Th2 balance. LMW-sericin also improved the differentiation of macrophages toward the M2 phenotype by significantly enhancing the expression of Arg-1, which is conducive to the repair of the inflammatory environment. Moreover, the gene expression of hASCs showed that LMW-sericin promoted the secretion of beneficial adhesion molecules that potentially activate the gene transcription of differentiation and migration in hASCs, as well as significantly enhanced the levels of PKCβ1, RhoA, and RasGFR1 as fruitful molecules in wound healing. These findings provide insights into LMW-sericin application as a potential biomaterial for wound management.
format Online
Article
Text
id pubmed-9343859
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93438592022-08-03 Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration Cherng, Juin-Hong Chang, Shu-Jen Chiu, Yaw-Kwan Chiu, Yu-Hsiang Fang, Tong-Jing Chen, Hsiang-Cheng Front Bioeng Biotechnol Bioengineering and Biotechnology Sericin, a waste product of the silk textile industry, has favorable physicochemical and biological properties. In this study, we extracted a low molecular weight (MW) sericin (LMW-sericin; below 10 kDa) by a performing high-temperature and high-pressure method and confirmed the MW using matrix-assisted laser desorption ionization-time of flight and liquid chromatography–mass spectrometry. Furthermore, we determined its biological effects on macrophages and human adipose stem cells (hASCs) as cell models to investigate the biocompatibility, immunomodulation behavior, and potential signaling pathway-related wound healing via analyses of gene expression of focal adhesion and human cytokines and chemokines using quantitative real-time polymerase chain reaction and cytokine assay. LMW-sericin showed good biocompatibility both in macrophages and hASCs. Macrophages cultured with 0.1 mg/ml LMW-sericin displayed an improved inflammatory response shown by the upregulation of CXCL9, IL12A, BMP7, and IL10, which developed Th1 and Th2 balance. LMW-sericin also improved the differentiation of macrophages toward the M2 phenotype by significantly enhancing the expression of Arg-1, which is conducive to the repair of the inflammatory environment. Moreover, the gene expression of hASCs showed that LMW-sericin promoted the secretion of beneficial adhesion molecules that potentially activate the gene transcription of differentiation and migration in hASCs, as well as significantly enhanced the levels of PKCβ1, RhoA, and RasGFR1 as fruitful molecules in wound healing. These findings provide insights into LMW-sericin application as a potential biomaterial for wound management. Frontiers Media S.A. 2022-07-19 /pmc/articles/PMC9343859/ /pubmed/35928949 http://dx.doi.org/10.3389/fbioe.2022.925197 Text en Copyright © 2022 Cherng, Chang, Chiu, Chiu, Fang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Cherng, Juin-Hong
Chang, Shu-Jen
Chiu, Yaw-Kwan
Chiu, Yu-Hsiang
Fang, Tong-Jing
Chen, Hsiang-Cheng
Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration
title Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration
title_full Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration
title_fullStr Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration
title_full_unstemmed Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration
title_short Low Molecular Weight Sericin Enhances the In Vitro of Immunological Modulation and Cell Migration
title_sort low molecular weight sericin enhances the in vitro of immunological modulation and cell migration
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343859/
https://www.ncbi.nlm.nih.gov/pubmed/35928949
http://dx.doi.org/10.3389/fbioe.2022.925197
work_keys_str_mv AT cherngjuinhong lowmolecularweightsericinenhancestheinvitroofimmunologicalmodulationandcellmigration
AT changshujen lowmolecularweightsericinenhancestheinvitroofimmunologicalmodulationandcellmigration
AT chiuyawkwan lowmolecularweightsericinenhancestheinvitroofimmunologicalmodulationandcellmigration
AT chiuyuhsiang lowmolecularweightsericinenhancestheinvitroofimmunologicalmodulationandcellmigration
AT fangtongjing lowmolecularweightsericinenhancestheinvitroofimmunologicalmodulationandcellmigration
AT chenhsiangcheng lowmolecularweightsericinenhancestheinvitroofimmunologicalmodulationandcellmigration