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Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae

Replicative lifespan, a measure of the number of times that a yeast cell can divide before senescence, is one model for aging. Here, we provide a protocol for enrichment of yeast as a function of replicative age using a miniature chemostat aging device (mCAD). This protocol allows for isolation of q...

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Detalles Bibliográficos
Autores principales: Yang, Emily J., Pon, Liza A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344026/
https://www.ncbi.nlm.nih.gov/pubmed/35928001
http://dx.doi.org/10.1016/j.xpro.2022.101599