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Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae
Replicative lifespan, a measure of the number of times that a yeast cell can divide before senescence, is one model for aging. Here, we provide a protocol for enrichment of yeast as a function of replicative age using a miniature chemostat aging device (mCAD). This protocol allows for isolation of q...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344026/ https://www.ncbi.nlm.nih.gov/pubmed/35928001 http://dx.doi.org/10.1016/j.xpro.2022.101599 |