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Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching

This protocol describes the use of fluorescence recovery after photobleaching (FRAP) to investigate the dynamics of Matrin-3 (MATR3) condensates in live budding yeast. We detail how to generate yeast strains containing MATR3 with an enhanced green fluorescent protein (eGFP) tag and induce MATR3-eGFP...

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Detalles Bibliográficos
Autores principales:	Sprunger, Macy L., Jackrel, Meredith E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344031/ https://www.ncbi.nlm.nih.gov/pubmed/35928002 http://dx.doi.org/10.1016/j.xpro.2022.101592

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