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Upregulation of CCNB2 and Its Perspective Mechanisms in Cerebral Ischemic Stroke and All Subtypes of Lung Cancer: A Comprehensive Study

Cyclin B2 (CCNB2) belongs to type B cell cycle family protein, which is located on chromosome 15q22, and it binds to cyclin-dependent kinases (CDKs) to regulate their activities. In this study, 103 high-throughput datasets related to all subtypes of lung cancer (LC) and cerebral ischemic stroke (CIS...

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Autores principales: Li, Ming-Jie, Yan, Shi-Bai, Chen, Gang, Li, Guo-Sheng, Yang, Yue, Wei, Tao, He, De-Shen, Yang, Zhen, Cen, Geng-Yu, Wang, Jun, Liu, Liu-Yu, Liang, Zhi-Jian, Chen, Li, Yin, Bin-Tong, Xu, Ruo-Xiang, Huang, Zhi-Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344069/
https://www.ncbi.nlm.nih.gov/pubmed/35928585
http://dx.doi.org/10.3389/fnint.2022.854540
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author Li, Ming-Jie
Yan, Shi-Bai
Chen, Gang
Li, Guo-Sheng
Yang, Yue
Wei, Tao
He, De-Shen
Yang, Zhen
Cen, Geng-Yu
Wang, Jun
Liu, Liu-Yu
Liang, Zhi-Jian
Chen, Li
Yin, Bin-Tong
Xu, Ruo-Xiang
Huang, Zhi-Guang
author_facet Li, Ming-Jie
Yan, Shi-Bai
Chen, Gang
Li, Guo-Sheng
Yang, Yue
Wei, Tao
He, De-Shen
Yang, Zhen
Cen, Geng-Yu
Wang, Jun
Liu, Liu-Yu
Liang, Zhi-Jian
Chen, Li
Yin, Bin-Tong
Xu, Ruo-Xiang
Huang, Zhi-Guang
author_sort Li, Ming-Jie
collection PubMed
description Cyclin B2 (CCNB2) belongs to type B cell cycle family protein, which is located on chromosome 15q22, and it binds to cyclin-dependent kinases (CDKs) to regulate their activities. In this study, 103 high-throughput datasets related to all subtypes of lung cancer (LC) and cerebral ischemic stroke (CIS) with the data of CCNB2 expression were collected. The analysis of standard mean deviation (SMD) and summary receiver operating characteristic (SROC) reflecting expression status demonstrated significant up-regulation of CCNB2 in LC and CIS (Lung adenocarcinoma: SMD = 1.40, 95%CI [0.98–1.83], SROC = 0.92, 95%CI [0.89–0.94]. Lung squamous cell carcinoma: SMD = 2.56, 95%CI [1.64–3.48]. SROC = 0.97, 95%CI [0.95–0.98]. Lung small cell carcinoma: SMD = 3.01, 95%CI [2.01–4.01]. SROC = 0.98, 95%CI [0.97–0.99]. CIS: SMD = 0.29, 95%CI [0.05–0.53], SROC = 0.68, 95%CI [0.63–0.71]). Simultaneously, protein-protein interaction (PPI) analysis indicated that CCNB2 is the hub molecule of crossed high-expressed genes in CIS and LC. Through Multiscale embedded gene co-expression network analysis (MEGENA), a gene module of CIS including 76 genes was obtained and function enrichment analysis of the CCNB2 module genes implied that CCNB2 may participate in the processes in the formation of CIS and tissue damage caused by CIS, such as “cell cycle,” “protein kinase activity,” and “glycosphingolipid biosynthesis.” Afterward, via single-cell RNA-seq analysis, CCNB2 was found up-regulated on GABAergic neurons in brain organoids as well as T cells expressing proliferative molecules in LUAD. Concurrently, the expression of CCNB2 distributed similarly to TOP2A as a module marker of cell proliferation in cell cluster. These findings can help in the field of the pathogenesis of LC-related CIS and neuron repair after CIS damage.
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spelling pubmed-93440692022-08-03 Upregulation of CCNB2 and Its Perspective Mechanisms in Cerebral Ischemic Stroke and All Subtypes of Lung Cancer: A Comprehensive Study Li, Ming-Jie Yan, Shi-Bai Chen, Gang Li, Guo-Sheng Yang, Yue Wei, Tao He, De-Shen Yang, Zhen Cen, Geng-Yu Wang, Jun Liu, Liu-Yu Liang, Zhi-Jian Chen, Li Yin, Bin-Tong Xu, Ruo-Xiang Huang, Zhi-Guang Front Integr Neurosci Neuroscience Cyclin B2 (CCNB2) belongs to type B cell cycle family protein, which is located on chromosome 15q22, and it binds to cyclin-dependent kinases (CDKs) to regulate their activities. In this study, 103 high-throughput datasets related to all subtypes of lung cancer (LC) and cerebral ischemic stroke (CIS) with the data of CCNB2 expression were collected. The analysis of standard mean deviation (SMD) and summary receiver operating characteristic (SROC) reflecting expression status demonstrated significant up-regulation of CCNB2 in LC and CIS (Lung adenocarcinoma: SMD = 1.40, 95%CI [0.98–1.83], SROC = 0.92, 95%CI [0.89–0.94]. Lung squamous cell carcinoma: SMD = 2.56, 95%CI [1.64–3.48]. SROC = 0.97, 95%CI [0.95–0.98]. Lung small cell carcinoma: SMD = 3.01, 95%CI [2.01–4.01]. SROC = 0.98, 95%CI [0.97–0.99]. CIS: SMD = 0.29, 95%CI [0.05–0.53], SROC = 0.68, 95%CI [0.63–0.71]). Simultaneously, protein-protein interaction (PPI) analysis indicated that CCNB2 is the hub molecule of crossed high-expressed genes in CIS and LC. Through Multiscale embedded gene co-expression network analysis (MEGENA), a gene module of CIS including 76 genes was obtained and function enrichment analysis of the CCNB2 module genes implied that CCNB2 may participate in the processes in the formation of CIS and tissue damage caused by CIS, such as “cell cycle,” “protein kinase activity,” and “glycosphingolipid biosynthesis.” Afterward, via single-cell RNA-seq analysis, CCNB2 was found up-regulated on GABAergic neurons in brain organoids as well as T cells expressing proliferative molecules in LUAD. Concurrently, the expression of CCNB2 distributed similarly to TOP2A as a module marker of cell proliferation in cell cluster. These findings can help in the field of the pathogenesis of LC-related CIS and neuron repair after CIS damage. Frontiers Media S.A. 2022-07-19 /pmc/articles/PMC9344069/ /pubmed/35928585 http://dx.doi.org/10.3389/fnint.2022.854540 Text en Copyright © 2022 Li, Yan, Chen, Li, Yang, Wei, He, Yang, Cen, Wang, Liu, Liang, Chen, Yin, Xu and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Li, Ming-Jie
Yan, Shi-Bai
Chen, Gang
Li, Guo-Sheng
Yang, Yue
Wei, Tao
He, De-Shen
Yang, Zhen
Cen, Geng-Yu
Wang, Jun
Liu, Liu-Yu
Liang, Zhi-Jian
Chen, Li
Yin, Bin-Tong
Xu, Ruo-Xiang
Huang, Zhi-Guang
Upregulation of CCNB2 and Its Perspective Mechanisms in Cerebral Ischemic Stroke and All Subtypes of Lung Cancer: A Comprehensive Study
title Upregulation of CCNB2 and Its Perspective Mechanisms in Cerebral Ischemic Stroke and All Subtypes of Lung Cancer: A Comprehensive Study
title_full Upregulation of CCNB2 and Its Perspective Mechanisms in Cerebral Ischemic Stroke and All Subtypes of Lung Cancer: A Comprehensive Study
title_fullStr Upregulation of CCNB2 and Its Perspective Mechanisms in Cerebral Ischemic Stroke and All Subtypes of Lung Cancer: A Comprehensive Study
title_full_unstemmed Upregulation of CCNB2 and Its Perspective Mechanisms in Cerebral Ischemic Stroke and All Subtypes of Lung Cancer: A Comprehensive Study
title_short Upregulation of CCNB2 and Its Perspective Mechanisms in Cerebral Ischemic Stroke and All Subtypes of Lung Cancer: A Comprehensive Study
title_sort upregulation of ccnb2 and its perspective mechanisms in cerebral ischemic stroke and all subtypes of lung cancer: a comprehensive study
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344069/
https://www.ncbi.nlm.nih.gov/pubmed/35928585
http://dx.doi.org/10.3389/fnint.2022.854540
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