Cargando…

Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs

This experiment evaluated the impacts of essential oils (EO) and protease (PRO), independently or in combination, on growth performance, antioxidation, inflammation and intestinal function of weaned pigs. One hundred and sixty weaned pigs (21 d of age, BW of 6.74 ± 0.20 kg) were randomly divided int...

Descripción completa

Detalles Bibliográficos
Autores principales: Peng, Xie, Zhou, Qiang, Wu, Cheng, Zhao, Jian, Tan, Quan, He, Ying, Hu, Liang, Fang, Zhengfeng, Lin, Yan, Xu, Shengyu, Feng, Bin, Li, Jian, Zhuo, Yong, Van Ginneken, Chris, Jiang, Xuemei, Wu, De, Che, Lianqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344314/
https://www.ncbi.nlm.nih.gov/pubmed/35949988
http://dx.doi.org/10.1016/j.aninu.2021.12.003
_version_ 1784761194842685440
author Peng, Xie
Zhou, Qiang
Wu, Cheng
Zhao, Jian
Tan, Quan
He, Ying
Hu, Liang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Li, Jian
Zhuo, Yong
Van Ginneken, Chris
Jiang, Xuemei
Wu, De
Che, Lianqiang
author_facet Peng, Xie
Zhou, Qiang
Wu, Cheng
Zhao, Jian
Tan, Quan
He, Ying
Hu, Liang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Li, Jian
Zhuo, Yong
Van Ginneken, Chris
Jiang, Xuemei
Wu, De
Che, Lianqiang
author_sort Peng, Xie
collection PubMed
description This experiment evaluated the impacts of essential oils (EO) and protease (PRO), independently or in combination, on growth performance, antioxidation, inflammation and intestinal function of weaned pigs. One hundred and sixty weaned pigs (21 d of age, BW of 6.74 ± 0.20 kg) were randomly divided into 4 treatments with 8 replicate pens of 5 pigs per pen. Dietary treatments included the following: 1) control diet (CON), 2) CON with 300 mg/kg essential oils (EO), 3) CON with 500 mg/kg protease (PRO), 4) CON with 300 mg/kg essential oil and 500 mg/kg protease (EO + PRO). On d 8, one pig from each pen was selected for sampling. The remaining pigs were fed for an additional week and growth performance was monitored during this period. Dietary treatments had no marked effects (P > 0.05) on the growth performance of pigs. However, pigs receiving EO diet had higher (P < 0.05) serum glutathione peroxidase (GSH-Px) activity, and tended to decrease (P = 0.063) serum concentration of tumor necrosis factor-α (TNF-α). In addition, pigs receiving EO diet had higher (P < 0.05) abundances of phylum Actinobacteria, and genera Bifidobacterium, and lower (P < 0.05) phylum Bacteroidetes and genera Alloprevotella in colonic digesta. Pigs receiving PRO diet decreased (P < 0.05) the serum concentration of malondialdehyde (MDA) and diamine oxidase activity, increased (P < 0.05) the villus height and the ratio of villus height to crypt depth in duodenum, increased sucrase activity in jejunal mucosa, and also increased the abundance of phylum Actinobacteria in colonic digesta. Furthermore, the synergistic effects of EO and PRO was observed (P < 0.05) for pigs with decreasing serum TNF-α concentration and increasing serum GSH-Px activity. Collectively, the results indicated that dietary supplementation of EO and PRO had no significant effects on growth performance of weaned pigs. EO diet appeared to improve antioxidant activity and intestinal microbiota, while PRO diet improved intestinal morphology and digestive enzyme activity, and there was a synergistic effect of EO and PRO on reducing inflammatory parameters in weaned pigs.
format Online
Article
Text
id pubmed-9344314
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher KeAi Publishing
record_format MEDLINE/PubMed
spelling pubmed-93443142022-08-09 Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs Peng, Xie Zhou, Qiang Wu, Cheng Zhao, Jian Tan, Quan He, Ying Hu, Liang Fang, Zhengfeng Lin, Yan Xu, Shengyu Feng, Bin Li, Jian Zhuo, Yong Van Ginneken, Chris Jiang, Xuemei Wu, De Che, Lianqiang Anim Nutr Original Research Article This experiment evaluated the impacts of essential oils (EO) and protease (PRO), independently or in combination, on growth performance, antioxidation, inflammation and intestinal function of weaned pigs. One hundred and sixty weaned pigs (21 d of age, BW of 6.74 ± 0.20 kg) were randomly divided into 4 treatments with 8 replicate pens of 5 pigs per pen. Dietary treatments included the following: 1) control diet (CON), 2) CON with 300 mg/kg essential oils (EO), 3) CON with 500 mg/kg protease (PRO), 4) CON with 300 mg/kg essential oil and 500 mg/kg protease (EO + PRO). On d 8, one pig from each pen was selected for sampling. The remaining pigs were fed for an additional week and growth performance was monitored during this period. Dietary treatments had no marked effects (P > 0.05) on the growth performance of pigs. However, pigs receiving EO diet had higher (P < 0.05) serum glutathione peroxidase (GSH-Px) activity, and tended to decrease (P = 0.063) serum concentration of tumor necrosis factor-α (TNF-α). In addition, pigs receiving EO diet had higher (P < 0.05) abundances of phylum Actinobacteria, and genera Bifidobacterium, and lower (P < 0.05) phylum Bacteroidetes and genera Alloprevotella in colonic digesta. Pigs receiving PRO diet decreased (P < 0.05) the serum concentration of malondialdehyde (MDA) and diamine oxidase activity, increased (P < 0.05) the villus height and the ratio of villus height to crypt depth in duodenum, increased sucrase activity in jejunal mucosa, and also increased the abundance of phylum Actinobacteria in colonic digesta. Furthermore, the synergistic effects of EO and PRO was observed (P < 0.05) for pigs with decreasing serum TNF-α concentration and increasing serum GSH-Px activity. Collectively, the results indicated that dietary supplementation of EO and PRO had no significant effects on growth performance of weaned pigs. EO diet appeared to improve antioxidant activity and intestinal microbiota, while PRO diet improved intestinal morphology and digestive enzyme activity, and there was a synergistic effect of EO and PRO on reducing inflammatory parameters in weaned pigs. KeAi Publishing 2021-12-29 /pmc/articles/PMC9344314/ /pubmed/35949988 http://dx.doi.org/10.1016/j.aninu.2021.12.003 Text en © 2022 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
Peng, Xie
Zhou, Qiang
Wu, Cheng
Zhao, Jian
Tan, Quan
He, Ying
Hu, Liang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Li, Jian
Zhuo, Yong
Van Ginneken, Chris
Jiang, Xuemei
Wu, De
Che, Lianqiang
Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs
title Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs
title_full Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs
title_fullStr Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs
title_full_unstemmed Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs
title_short Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs
title_sort effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344314/
https://www.ncbi.nlm.nih.gov/pubmed/35949988
http://dx.doi.org/10.1016/j.aninu.2021.12.003
work_keys_str_mv AT pengxie effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT zhouqiang effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT wucheng effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT zhaojian effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT tanquan effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT heying effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT huliang effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT fangzhengfeng effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT linyan effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT xushengyu effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT fengbin effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT lijian effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT zhuoyong effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT vanginnekenchris effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT jiangxuemei effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT wude effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs
AT chelianqiang effectsofdietarysupplementationwithessentialoilsandproteaseongrowthperformanceantioxidationinflammationandintestinalfunctionofweanedpigs