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CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters

Acute flaccid paralysis (AFP) surveillance has been used to identify polio cases and target vaccination campaigns since the inception of the Global Poliovirus Eradication Initiative (GPEI) in 1988. To date, only Afghanistan and Pakistan have failed to interrupt wild poliovirus transmission. Circulat...

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Autores principales: Belgasmi, Hanen, Miles, Stacey Jeffries, Sayyad, Leanna, Wong, Kimberly, Harrington, Chelsea, Gerloff, Nancy, Coulliette-Salmond, Angela D, Guntapong, Ratigorn, Tacharoenmuang, Ratana, Ayutthaya, Apiradee Isarangkul Na, Apostol, Lea Necitas G., Valencia, Ma.Anne-Lesley D., Burns, Cara C., Benito, Gloria-Rey, Vega, Everardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344547/
https://www.ncbi.nlm.nih.gov/pubmed/35928599
http://dx.doi.org/10.3389/fenvs.2022.914387
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author Belgasmi, Hanen
Miles, Stacey Jeffries
Sayyad, Leanna
Wong, Kimberly
Harrington, Chelsea
Gerloff, Nancy
Coulliette-Salmond, Angela D
Guntapong, Ratigorn
Tacharoenmuang, Ratana
Ayutthaya, Apiradee Isarangkul Na
Apostol, Lea Necitas G.
Valencia, Ma.Anne-Lesley D.
Burns, Cara C.
Benito, Gloria-Rey
Vega, Everardo
author_facet Belgasmi, Hanen
Miles, Stacey Jeffries
Sayyad, Leanna
Wong, Kimberly
Harrington, Chelsea
Gerloff, Nancy
Coulliette-Salmond, Angela D
Guntapong, Ratigorn
Tacharoenmuang, Ratana
Ayutthaya, Apiradee Isarangkul Na
Apostol, Lea Necitas G.
Valencia, Ma.Anne-Lesley D.
Burns, Cara C.
Benito, Gloria-Rey
Vega, Everardo
author_sort Belgasmi, Hanen
collection PubMed
description Acute flaccid paralysis (AFP) surveillance has been used to identify polio cases and target vaccination campaigns since the inception of the Global Poliovirus Eradication Initiative (GPEI) in 1988. To date, only Afghanistan and Pakistan have failed to interrupt wild poliovirus transmission. Circulation of vaccine-derived polioviruses (VDPV) continues to be a problem in high-risk areas of the Eastern Mediterranean, African, and Southeast Asian regions. Environmental surveillance (ES) is an important adjunct to AFP surveillance, helping to identify circulating polioviruses in problematic areas. Stools from AFP cases and contacts (>200,000 specimens/year) and ES samples (>642 sites) are referred to 146 laboratories in the Global Polio Laboratory Network (GPLN) for testing. Although most World Health Organization supported laboratories use the two-phase separation method due to its simplicity and effectiveness, alternative simple, widely available, and cost-effective methods are needed. The CAFÉ (Concentration and Filtration Elution) method was developed from existing filtration methods to handle any type of sewage or residual waters. At $10–20 US per sample for consumable materials, CAFÉ is cost effective, and all equipment and reagents are readily available from markets and suppliers globally. The report describes the results from a parallel study of CAFÉ method with the standard two-phase separation method. The study was performed with samples collected from five countries (Guatemala, Haïti, Thailand, Papua New Guinea, and the Philippines), run in three laboratories–(United States, Thailand and in the Philippines) to account for regional and sample-to-sample variability. Samples from each site were divided into two 500 ml aliquots and processed by both methods, with no other additional concentration or manipulation. The results of 338 parallel-tested samples show that the CAFÉ method is more sensitive than the two-phase separation method for detection of non-polio enteroviruses (p-value < 0.0001) and performed as well as the two-phase separation method for polioviruses detection with no significant difference (p-value > 0.05). The CAFÉ method is a robust, sensitive, and cost-effective method for isolating enteroviruses from residual waters.
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spelling pubmed-93445472023-07-04 CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters Belgasmi, Hanen Miles, Stacey Jeffries Sayyad, Leanna Wong, Kimberly Harrington, Chelsea Gerloff, Nancy Coulliette-Salmond, Angela D Guntapong, Ratigorn Tacharoenmuang, Ratana Ayutthaya, Apiradee Isarangkul Na Apostol, Lea Necitas G. Valencia, Ma.Anne-Lesley D. Burns, Cara C. Benito, Gloria-Rey Vega, Everardo Front Environ Sci Article Acute flaccid paralysis (AFP) surveillance has been used to identify polio cases and target vaccination campaigns since the inception of the Global Poliovirus Eradication Initiative (GPEI) in 1988. To date, only Afghanistan and Pakistan have failed to interrupt wild poliovirus transmission. Circulation of vaccine-derived polioviruses (VDPV) continues to be a problem in high-risk areas of the Eastern Mediterranean, African, and Southeast Asian regions. Environmental surveillance (ES) is an important adjunct to AFP surveillance, helping to identify circulating polioviruses in problematic areas. Stools from AFP cases and contacts (>200,000 specimens/year) and ES samples (>642 sites) are referred to 146 laboratories in the Global Polio Laboratory Network (GPLN) for testing. Although most World Health Organization supported laboratories use the two-phase separation method due to its simplicity and effectiveness, alternative simple, widely available, and cost-effective methods are needed. The CAFÉ (Concentration and Filtration Elution) method was developed from existing filtration methods to handle any type of sewage or residual waters. At $10–20 US per sample for consumable materials, CAFÉ is cost effective, and all equipment and reagents are readily available from markets and suppliers globally. The report describes the results from a parallel study of CAFÉ method with the standard two-phase separation method. The study was performed with samples collected from five countries (Guatemala, Haïti, Thailand, Papua New Guinea, and the Philippines), run in three laboratories–(United States, Thailand and in the Philippines) to account for regional and sample-to-sample variability. Samples from each site were divided into two 500 ml aliquots and processed by both methods, with no other additional concentration or manipulation. The results of 338 parallel-tested samples show that the CAFÉ method is more sensitive than the two-phase separation method for detection of non-polio enteroviruses (p-value < 0.0001) and performed as well as the two-phase separation method for polioviruses detection with no significant difference (p-value > 0.05). The CAFÉ method is a robust, sensitive, and cost-effective method for isolating enteroviruses from residual waters. 2022-07-04 /pmc/articles/PMC9344547/ /pubmed/35928599 http://dx.doi.org/10.3389/fenvs.2022.914387 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Article
Belgasmi, Hanen
Miles, Stacey Jeffries
Sayyad, Leanna
Wong, Kimberly
Harrington, Chelsea
Gerloff, Nancy
Coulliette-Salmond, Angela D
Guntapong, Ratigorn
Tacharoenmuang, Ratana
Ayutthaya, Apiradee Isarangkul Na
Apostol, Lea Necitas G.
Valencia, Ma.Anne-Lesley D.
Burns, Cara C.
Benito, Gloria-Rey
Vega, Everardo
CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters
title CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters
title_full CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters
title_fullStr CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters
title_full_unstemmed CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters
title_short CaFÉ: A Sensitive, Low-Cost Filtration Method for Detecting Polioviruses and Other Enteroviruses in Residual Waters
title_sort café: a sensitive, low-cost filtration method for detecting polioviruses and other enteroviruses in residual waters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344547/
https://www.ncbi.nlm.nih.gov/pubmed/35928599
http://dx.doi.org/10.3389/fenvs.2022.914387
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