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Utility of serum indices in a particular case of serum protein electrophoresis

Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been large...

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Autores principales: Daves, Massimo, Piccin, Andrea, Vicidomini, Cristina, De Luisi, Antonia, Mega, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Croatian Society of Medical Biochemistry and Laboratory Medicine 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344864/
https://www.ncbi.nlm.nih.gov/pubmed/35966258
http://dx.doi.org/10.11613/BM.2022.030802
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author Daves, Massimo
Piccin, Andrea
Vicidomini, Cristina
De Luisi, Antonia
Mega, Andrea
author_facet Daves, Massimo
Piccin, Andrea
Vicidomini, Cristina
De Luisi, Antonia
Mega, Andrea
author_sort Daves, Massimo
collection PubMed
description Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been largely improved by CZE. Capillary electrophoresis estimates CM more accurately, because absence of variation due to different dye binding affinities of proteins as instead seen with agarose gel electrophoresis. However, interferences can be present in CZE. This occurs because all substances absorbing at 200 nm can be identified. Recognition and handling of specimens exhibiting such interferences is essential to ensure accurate diagnostic and patient safety. We herein report on an unusual case of serum protein electrophoresis, to highlight that laboratory staff must be aware of and familiarise with the information provided by laboratory instruments. For example, in the case of serum indices, about specimen quality.
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spelling pubmed-93448642022-08-11 Utility of serum indices in a particular case of serum protein electrophoresis Daves, Massimo Piccin, Andrea Vicidomini, Cristina De Luisi, Antonia Mega, Andrea Biochem Med (Zagreb) Case Reports Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been largely improved by CZE. Capillary electrophoresis estimates CM more accurately, because absence of variation due to different dye binding affinities of proteins as instead seen with agarose gel electrophoresis. However, interferences can be present in CZE. This occurs because all substances absorbing at 200 nm can be identified. Recognition and handling of specimens exhibiting such interferences is essential to ensure accurate diagnostic and patient safety. We herein report on an unusual case of serum protein electrophoresis, to highlight that laboratory staff must be aware of and familiarise with the information provided by laboratory instruments. For example, in the case of serum indices, about specimen quality. Croatian Society of Medical Biochemistry and Laboratory Medicine 2022-08-05 2022-10-01 /pmc/articles/PMC9344864/ /pubmed/35966258 http://dx.doi.org/10.11613/BM.2022.030802 Text en Croatian Society of Medical Biochemistry and Laboratory Medicine. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Reports
Daves, Massimo
Piccin, Andrea
Vicidomini, Cristina
De Luisi, Antonia
Mega, Andrea
Utility of serum indices in a particular case of serum protein electrophoresis
title Utility of serum indices in a particular case of serum protein electrophoresis
title_full Utility of serum indices in a particular case of serum protein electrophoresis
title_fullStr Utility of serum indices in a particular case of serum protein electrophoresis
title_full_unstemmed Utility of serum indices in a particular case of serum protein electrophoresis
title_short Utility of serum indices in a particular case of serum protein electrophoresis
title_sort utility of serum indices in a particular case of serum protein electrophoresis
topic Case Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344864/
https://www.ncbi.nlm.nih.gov/pubmed/35966258
http://dx.doi.org/10.11613/BM.2022.030802
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