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Utility of serum indices in a particular case of serum protein electrophoresis
Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been large...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Croatian Society of Medical Biochemistry and Laboratory Medicine
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344864/ https://www.ncbi.nlm.nih.gov/pubmed/35966258 http://dx.doi.org/10.11613/BM.2022.030802 |
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author | Daves, Massimo Piccin, Andrea Vicidomini, Cristina De Luisi, Antonia Mega, Andrea |
author_facet | Daves, Massimo Piccin, Andrea Vicidomini, Cristina De Luisi, Antonia Mega, Andrea |
author_sort | Daves, Massimo |
collection | PubMed |
description | Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been largely improved by CZE. Capillary electrophoresis estimates CM more accurately, because absence of variation due to different dye binding affinities of proteins as instead seen with agarose gel electrophoresis. However, interferences can be present in CZE. This occurs because all substances absorbing at 200 nm can be identified. Recognition and handling of specimens exhibiting such interferences is essential to ensure accurate diagnostic and patient safety. We herein report on an unusual case of serum protein electrophoresis, to highlight that laboratory staff must be aware of and familiarise with the information provided by laboratory instruments. For example, in the case of serum indices, about specimen quality. |
format | Online Article Text |
id | pubmed-9344864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Croatian Society of Medical Biochemistry and Laboratory Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-93448642022-08-11 Utility of serum indices in a particular case of serum protein electrophoresis Daves, Massimo Piccin, Andrea Vicidomini, Cristina De Luisi, Antonia Mega, Andrea Biochem Med (Zagreb) Case Reports Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been largely improved by CZE. Capillary electrophoresis estimates CM more accurately, because absence of variation due to different dye binding affinities of proteins as instead seen with agarose gel electrophoresis. However, interferences can be present in CZE. This occurs because all substances absorbing at 200 nm can be identified. Recognition and handling of specimens exhibiting such interferences is essential to ensure accurate diagnostic and patient safety. We herein report on an unusual case of serum protein electrophoresis, to highlight that laboratory staff must be aware of and familiarise with the information provided by laboratory instruments. For example, in the case of serum indices, about specimen quality. Croatian Society of Medical Biochemistry and Laboratory Medicine 2022-08-05 2022-10-01 /pmc/articles/PMC9344864/ /pubmed/35966258 http://dx.doi.org/10.11613/BM.2022.030802 Text en Croatian Society of Medical Biochemistry and Laboratory Medicine. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Case Reports Daves, Massimo Piccin, Andrea Vicidomini, Cristina De Luisi, Antonia Mega, Andrea Utility of serum indices in a particular case of serum protein electrophoresis |
title | Utility of serum indices in a particular case of serum protein electrophoresis |
title_full | Utility of serum indices in a particular case of serum protein electrophoresis |
title_fullStr | Utility of serum indices in a particular case of serum protein electrophoresis |
title_full_unstemmed | Utility of serum indices in a particular case of serum protein electrophoresis |
title_short | Utility of serum indices in a particular case of serum protein electrophoresis |
title_sort | utility of serum indices in a particular case of serum protein electrophoresis |
topic | Case Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344864/ https://www.ncbi.nlm.nih.gov/pubmed/35966258 http://dx.doi.org/10.11613/BM.2022.030802 |
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