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Drill-Hole Bone Defects in Animal Models of Bone Healing: Protocol for a Systematic Review

BACKGROUND: Bone fractures are common conditions of the musculoskeletal system. Several animal models of bone fractures have been established to help elucidate the complex process of bone healing. In the last decades, drill-hole bone defects have emerged as a method to study bone healing. Animal mod...

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Autores principales: Bromer, Frederik Duch, Brent, Mikkel Bo, Thomsen, Jesper Skovhus, Brüel, Annemarie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: JMIR Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345022/
https://www.ncbi.nlm.nih.gov/pubmed/35849443
http://dx.doi.org/10.2196/34887
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author Bromer, Frederik Duch
Brent, Mikkel Bo
Thomsen, Jesper Skovhus
Brüel, Annemarie
author_facet Bromer, Frederik Duch
Brent, Mikkel Bo
Thomsen, Jesper Skovhus
Brüel, Annemarie
author_sort Bromer, Frederik Duch
collection PubMed
description BACKGROUND: Bone fractures are common conditions of the musculoskeletal system. Several animal models of bone fractures have been established to help elucidate the complex process of bone healing. In the last decades, drill-hole bone defects have emerged as a method to study bone healing. Animal models of drill-hole defects are easy to standardize and do not require external fixation of the bone. However, current studies of drill-hole bone defects lack detailed descriptions of techniques and interstudy standardization. OBJECTIVE: This systematic review aims to present a detailed description of the different methods used to induce drill-hole bone defects in long bones of laboratory animals and to provide a comprehensive overview of their methodology and potential for investigation of bone healing. METHODS: A systematic search of PubMed and Embase will be performed of abstracts containing variations of the following four keywords: “long bone,” “drill-hole,” “regeneration,” and “animal model.” Abstract screening and full-text screening will be performed independently by 2 reviewers, and data will be extracted to a predesigned extraction protocol. The primary outcome of the included studies is the technique used to create the drill-hole bone defect, and secondary outcomes are any measurements or analyses of bone defect and regeneration. A narrative synthesis will be used to present the primary outcome, while information on secondary outcomes will be displayed graphically. The study protocol follows the PRISMA-P (Preferred Reporting Items for Systematic Review and Meta-analysis Protocols) guidelines. RESULTS: Abstract and full-text screening is ongoing and is expected to be completed by October 2022. Data extraction will commence immediately after, and the manuscript is expected to be completed by December 2023. The systematic review will follow the PRISMA statement. CONCLUSIONS: The strength of this systematic review is that it provides a comprehensive methodological overview of the different drill-hole methods and their advantages and disadvantages. This will assist researchers in choosing which model to use when studying different aspects of bone healing. TRIAL REGISTRATION: International Prospective Register of Systematic Reviews CRD42020213076; https://tinyurl.com/bp56wdwe INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): PRR1-10.2196/34887
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spelling pubmed-93450222022-08-03 Drill-Hole Bone Defects in Animal Models of Bone Healing: Protocol for a Systematic Review Bromer, Frederik Duch Brent, Mikkel Bo Thomsen, Jesper Skovhus Brüel, Annemarie JMIR Res Protoc Protocol BACKGROUND: Bone fractures are common conditions of the musculoskeletal system. Several animal models of bone fractures have been established to help elucidate the complex process of bone healing. In the last decades, drill-hole bone defects have emerged as a method to study bone healing. Animal models of drill-hole defects are easy to standardize and do not require external fixation of the bone. However, current studies of drill-hole bone defects lack detailed descriptions of techniques and interstudy standardization. OBJECTIVE: This systematic review aims to present a detailed description of the different methods used to induce drill-hole bone defects in long bones of laboratory animals and to provide a comprehensive overview of their methodology and potential for investigation of bone healing. METHODS: A systematic search of PubMed and Embase will be performed of abstracts containing variations of the following four keywords: “long bone,” “drill-hole,” “regeneration,” and “animal model.” Abstract screening and full-text screening will be performed independently by 2 reviewers, and data will be extracted to a predesigned extraction protocol. The primary outcome of the included studies is the technique used to create the drill-hole bone defect, and secondary outcomes are any measurements or analyses of bone defect and regeneration. A narrative synthesis will be used to present the primary outcome, while information on secondary outcomes will be displayed graphically. The study protocol follows the PRISMA-P (Preferred Reporting Items for Systematic Review and Meta-analysis Protocols) guidelines. RESULTS: Abstract and full-text screening is ongoing and is expected to be completed by October 2022. Data extraction will commence immediately after, and the manuscript is expected to be completed by December 2023. The systematic review will follow the PRISMA statement. CONCLUSIONS: The strength of this systematic review is that it provides a comprehensive methodological overview of the different drill-hole methods and their advantages and disadvantages. This will assist researchers in choosing which model to use when studying different aspects of bone healing. TRIAL REGISTRATION: International Prospective Register of Systematic Reviews CRD42020213076; https://tinyurl.com/bp56wdwe INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): PRR1-10.2196/34887 JMIR Publications 2022-07-18 /pmc/articles/PMC9345022/ /pubmed/35849443 http://dx.doi.org/10.2196/34887 Text en ©Frederik Duch Bromer, Mikkel Bo Brent, Jesper Skovhus Thomsen, Annemarie Brüel. Originally published in JMIR Research Protocols (https://www.researchprotocols.org), 18.07.2022. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Research Protocols, is properly cited. The complete bibliographic information, a link to the original publication on https://www.researchprotocols.org, as well as this copyright and license information must be included.
spellingShingle Protocol
Bromer, Frederik Duch
Brent, Mikkel Bo
Thomsen, Jesper Skovhus
Brüel, Annemarie
Drill-Hole Bone Defects in Animal Models of Bone Healing: Protocol for a Systematic Review
title Drill-Hole Bone Defects in Animal Models of Bone Healing: Protocol for a Systematic Review
title_full Drill-Hole Bone Defects in Animal Models of Bone Healing: Protocol for a Systematic Review
title_fullStr Drill-Hole Bone Defects in Animal Models of Bone Healing: Protocol for a Systematic Review
title_full_unstemmed Drill-Hole Bone Defects in Animal Models of Bone Healing: Protocol for a Systematic Review
title_short Drill-Hole Bone Defects in Animal Models of Bone Healing: Protocol for a Systematic Review
title_sort drill-hole bone defects in animal models of bone healing: protocol for a systematic review
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345022/
https://www.ncbi.nlm.nih.gov/pubmed/35849443
http://dx.doi.org/10.2196/34887
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