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The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats ( Rattus norvegicus)
Background: Prolonged activation of skeletal muscles causes a decrease in the production of fatigue. Exercise with strenuous intensity causes an increase in Reactive Oxygen Species (ROS). An increase in free radicals causes oxidative stress resulting in damage to cell function to mitochondrial dysfu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000 Research Limited
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345267/ https://www.ncbi.nlm.nih.gov/pubmed/35966961 http://dx.doi.org/10.12688/f1000research.54254.3 |
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author | Rusip, Gusbakti Ilyas, Syafrudin Lister, I. Nyoman Ehrich Ginting, Chrismis N. Mukti, Indra |
author_facet | Rusip, Gusbakti Ilyas, Syafrudin Lister, I. Nyoman Ehrich Ginting, Chrismis N. Mukti, Indra |
author_sort | Rusip, Gusbakti |
collection | PubMed |
description | Background: Prolonged activation of skeletal muscles causes a decrease in the production of fatigue. Exercise with strenuous intensity causes an increase in Reactive Oxygen Species (ROS). An increase in free radicals causes oxidative stress resulting in damage to cell function to mitochondrial dysfunction, and fatigue. This study aimed to determine the antioxidant potential of red dragon fruit (RDF) to delay fatigue due to oxidative stress, which improves cell function in mitochondria. Methods: 25 male rats ( Rattus norvegicus) aged three months were divided into five groups: Group K1 was N.A. (No Activity) but drinking and eating; Group K2 performed strenuous exercise without RDF treatment; Groups 3, 4, and 5 (P1, P2 and P3, respectively) performed strenuous exercise and were treated with 75 mg kg (-1).bw, 150 mg kg (-1).bw, and 300 mg kg (-1).bw of RDF extract, respectively. The exercise for the rats involved intense swimming for 20 minutes a day three time a week for fouweeks. Malondialdehyde (MDA) dan SOD was measured with the ELISA and histopathology for muscle soleus and lung tissue. Results: Strenuous exercise followed by RDF extract ingestion was compared for fatigue in terms of duration and time; before (24.55±1.38 minute) and after (95.31±7.82 minute) and led to a significant difference of 39% (p<0.01). The study also compared MDA before and after RDF extract ingestion in the K2 vs. the P1 group (p<0.05). At the same time, P2 differed more significantly (p<0.01). This indicated a spread of free radicals and featured histopathological damage of muscle cells. However, ingestion of RDF extract leads to improvement of soleus muscle cells; thus, repairs cell function, delaying fatigue. Conclusion: This study confirmed that strenuous exercise, which causes an increase in ROS, intensifies free radicals with RDF extract ingestion and declines oxidative stress, repairing cell function and delaying fatigue. |
format | Online Article Text |
id | pubmed-9345267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-93452672022-08-11 The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats ( Rattus norvegicus) Rusip, Gusbakti Ilyas, Syafrudin Lister, I. Nyoman Ehrich Ginting, Chrismis N. Mukti, Indra F1000Res Research Article Background: Prolonged activation of skeletal muscles causes a decrease in the production of fatigue. Exercise with strenuous intensity causes an increase in Reactive Oxygen Species (ROS). An increase in free radicals causes oxidative stress resulting in damage to cell function to mitochondrial dysfunction, and fatigue. This study aimed to determine the antioxidant potential of red dragon fruit (RDF) to delay fatigue due to oxidative stress, which improves cell function in mitochondria. Methods: 25 male rats ( Rattus norvegicus) aged three months were divided into five groups: Group K1 was N.A. (No Activity) but drinking and eating; Group K2 performed strenuous exercise without RDF treatment; Groups 3, 4, and 5 (P1, P2 and P3, respectively) performed strenuous exercise and were treated with 75 mg kg (-1).bw, 150 mg kg (-1).bw, and 300 mg kg (-1).bw of RDF extract, respectively. The exercise for the rats involved intense swimming for 20 minutes a day three time a week for fouweeks. Malondialdehyde (MDA) dan SOD was measured with the ELISA and histopathology for muscle soleus and lung tissue. Results: Strenuous exercise followed by RDF extract ingestion was compared for fatigue in terms of duration and time; before (24.55±1.38 minute) and after (95.31±7.82 minute) and led to a significant difference of 39% (p<0.01). The study also compared MDA before and after RDF extract ingestion in the K2 vs. the P1 group (p<0.05). At the same time, P2 differed more significantly (p<0.01). This indicated a spread of free radicals and featured histopathological damage of muscle cells. However, ingestion of RDF extract leads to improvement of soleus muscle cells; thus, repairs cell function, delaying fatigue. Conclusion: This study confirmed that strenuous exercise, which causes an increase in ROS, intensifies free radicals with RDF extract ingestion and declines oxidative stress, repairing cell function and delaying fatigue. F1000 Research Limited 2022-08-04 /pmc/articles/PMC9345267/ /pubmed/35966961 http://dx.doi.org/10.12688/f1000research.54254.3 Text en Copyright: © 2022 Rusip G et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Rusip, Gusbakti Ilyas, Syafrudin Lister, I. Nyoman Ehrich Ginting, Chrismis N. Mukti, Indra The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats ( Rattus norvegicus) |
title | The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats (
Rattus norvegicus) |
title_full | The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats (
Rattus norvegicus) |
title_fullStr | The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats (
Rattus norvegicus) |
title_full_unstemmed | The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats (
Rattus norvegicus) |
title_short | The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats (
Rattus norvegicus) |
title_sort | effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats (
rattus norvegicus) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345267/ https://www.ncbi.nlm.nih.gov/pubmed/35966961 http://dx.doi.org/10.12688/f1000research.54254.3 |
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