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Does Choosing Microfluidics for Sperm Sorting Offer an Advantage to Improve Clinical Pregnancies in Donor Egg Recipients?
BACKGROUND: Microfluidics (MF), an advanced sperm sorting technology results in the extraction of spermatozoa with higher DNA integrity and lower DNA damage compared to existing conventional sperm sorting methods. AIMS: The aim of the present study is to assess the efficiency of MF and to isolate th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345273/ https://www.ncbi.nlm.nih.gov/pubmed/35928460 http://dx.doi.org/10.4103/jhrs.jhrs_15_22 |
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author | Srinivas, Sapna Donthi, Suhasini Mettler, Anupama Deenadayal Tolani, Aarti Deenadayal Deenadayal, Mamata |
author_facet | Srinivas, Sapna Donthi, Suhasini Mettler, Anupama Deenadayal Tolani, Aarti Deenadayal Deenadayal, Mamata |
author_sort | Srinivas, Sapna |
collection | PubMed |
description | BACKGROUND: Microfluidics (MF), an advanced sperm sorting technology results in the extraction of spermatozoa with higher DNA integrity and lower DNA damage compared to existing conventional sperm sorting methods. AIMS: The aim of the present study is to assess the efficiency of MF and to isolate the best spermatozoa for intracytoplasmic sperm injection (ICSI) over the density gradient (DG) technique. STUDY SETTING AND DESIGN: We recruited couples who choose the oocyte donation programme for this study to eliminate confounding factors associated with oocyte quality. MATERIALS AND METHODS: Sperm was processed by MF (n = 180) and DG (n = 151). ICSI was performed and positive pregnancy, miscarriage and clinical pregnancy rates were compared. STATISTICAL ANALYSIS: All variables were analysed using Graph Pad Prism 5. The unpaired two-tailed t-test was used to assess the significance. A value of P < 0.05 was considered statistically significant. RESULTS: There was no significant difference in pregnancy rates between the groups. However, a clear demarcation is seen in terms of clinical pregnancy rates, where the DG group achieved higher clinical pregnancies (91.7%) compared to the MF group (80.7%). Further, we compared miscarriage rates and biochemical pregnancies, and found a significantly higher miscarriage and biochemical pregnancy rate in the MF group (14.5% and 4%, respectively) compared to the DG group (6% and 1%, respectively). CONCLUSIONS: Based on the available literature, we anticipated a higher clinical pregnancy rate with MF compared with conventional processing. Our results show MF does not have any add-on positive effect on clinical pregnancy rate. |
format | Online Article Text |
id | pubmed-9345273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-93452732022-08-03 Does Choosing Microfluidics for Sperm Sorting Offer an Advantage to Improve Clinical Pregnancies in Donor Egg Recipients? Srinivas, Sapna Donthi, Suhasini Mettler, Anupama Deenadayal Tolani, Aarti Deenadayal Deenadayal, Mamata J Hum Reprod Sci Original Article BACKGROUND: Microfluidics (MF), an advanced sperm sorting technology results in the extraction of spermatozoa with higher DNA integrity and lower DNA damage compared to existing conventional sperm sorting methods. AIMS: The aim of the present study is to assess the efficiency of MF and to isolate the best spermatozoa for intracytoplasmic sperm injection (ICSI) over the density gradient (DG) technique. STUDY SETTING AND DESIGN: We recruited couples who choose the oocyte donation programme for this study to eliminate confounding factors associated with oocyte quality. MATERIALS AND METHODS: Sperm was processed by MF (n = 180) and DG (n = 151). ICSI was performed and positive pregnancy, miscarriage and clinical pregnancy rates were compared. STATISTICAL ANALYSIS: All variables were analysed using Graph Pad Prism 5. The unpaired two-tailed t-test was used to assess the significance. A value of P < 0.05 was considered statistically significant. RESULTS: There was no significant difference in pregnancy rates between the groups. However, a clear demarcation is seen in terms of clinical pregnancy rates, where the DG group achieved higher clinical pregnancies (91.7%) compared to the MF group (80.7%). Further, we compared miscarriage rates and biochemical pregnancies, and found a significantly higher miscarriage and biochemical pregnancy rate in the MF group (14.5% and 4%, respectively) compared to the DG group (6% and 1%, respectively). CONCLUSIONS: Based on the available literature, we anticipated a higher clinical pregnancy rate with MF compared with conventional processing. Our results show MF does not have any add-on positive effect on clinical pregnancy rate. Wolters Kluwer - Medknow 2022 2022-06-30 /pmc/articles/PMC9345273/ /pubmed/35928460 http://dx.doi.org/10.4103/jhrs.jhrs_15_22 Text en Copyright: © 2022 Journal of Human Reproductive Sciences https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Srinivas, Sapna Donthi, Suhasini Mettler, Anupama Deenadayal Tolani, Aarti Deenadayal Deenadayal, Mamata Does Choosing Microfluidics for Sperm Sorting Offer an Advantage to Improve Clinical Pregnancies in Donor Egg Recipients? |
title | Does Choosing Microfluidics for Sperm Sorting Offer an Advantage to Improve Clinical Pregnancies in Donor Egg Recipients? |
title_full | Does Choosing Microfluidics for Sperm Sorting Offer an Advantage to Improve Clinical Pregnancies in Donor Egg Recipients? |
title_fullStr | Does Choosing Microfluidics for Sperm Sorting Offer an Advantage to Improve Clinical Pregnancies in Donor Egg Recipients? |
title_full_unstemmed | Does Choosing Microfluidics for Sperm Sorting Offer an Advantage to Improve Clinical Pregnancies in Donor Egg Recipients? |
title_short | Does Choosing Microfluidics for Sperm Sorting Offer an Advantage to Improve Clinical Pregnancies in Donor Egg Recipients? |
title_sort | does choosing microfluidics for sperm sorting offer an advantage to improve clinical pregnancies in donor egg recipients? |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345273/ https://www.ncbi.nlm.nih.gov/pubmed/35928460 http://dx.doi.org/10.4103/jhrs.jhrs_15_22 |
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