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Unveiling the Angiotensin-(1–7) Actions on the Urinary Bladder in Female Rats

Angiotensin-(1–7) is a peptide produced by different pathways, and regardless of the route, the angiotensin-converting enzyme 2 (ACE-2) is involved in one of the steps of its synthesis. Angiotensin-(1–7) binds to Mas receptors localized in different cells throughout the body. Whether angiotensin-(1–...

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Autores principales: Lamy, Gustavo B., Cafarchio, Eduardo M., do Vale, Bárbara, Antonio, Bruno B., Venancio, Daniel P., de Souza, Janaina S., Maciel, Rui M., Giannocco, Gisele, Silva Neto, Artur F., Oyama, Lila M., Aronsson, Patrik, Sato, Monica A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345415/
https://www.ncbi.nlm.nih.gov/pubmed/35928558
http://dx.doi.org/10.3389/fphys.2022.920636
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author Lamy, Gustavo B.
Cafarchio, Eduardo M.
do Vale, Bárbara
Antonio, Bruno B.
Venancio, Daniel P.
de Souza, Janaina S.
Maciel, Rui M.
Giannocco, Gisele
Silva Neto, Artur F.
Oyama, Lila M.
Aronsson, Patrik
Sato, Monica A.
author_facet Lamy, Gustavo B.
Cafarchio, Eduardo M.
do Vale, Bárbara
Antonio, Bruno B.
Venancio, Daniel P.
de Souza, Janaina S.
Maciel, Rui M.
Giannocco, Gisele
Silva Neto, Artur F.
Oyama, Lila M.
Aronsson, Patrik
Sato, Monica A.
author_sort Lamy, Gustavo B.
collection PubMed
description Angiotensin-(1–7) is a peptide produced by different pathways, and regardless of the route, the angiotensin-converting enzyme 2 (ACE-2) is involved in one of the steps of its synthesis. Angiotensin-(1–7) binds to Mas receptors localized in different cells throughout the body. Whether angiotensin-(1–7) exerts any action in the urinary bladder (UB) is still unknown. We investigated the effects of intravenous and topical (in situ) administration of angiotensin-(1–7) on intravesical pressure (IP) and cardiovascular variables. In addition, the Mas receptors and ACE-2 gene and protein expression were analyzed in the UB. Adult female Wistar rats were anesthetized with 2% isoflurane in 100% O(2) and submitted to the catheterization of the femoral artery and vein for mean arterial pressure (MAP) and heart rate (HR) recordings, and infusion of drugs, respectively. The renal blood flow was acquired using a Doppler flow probe placed around the left renal artery and the renal conductance (RC) was calculated as a ratio of Doppler shift (kHz) and MAP. The cannulation of the UB was performed for IP recording. We observed that angiotensin-(1–7) either administered intravenously [115.8 ± 28.6% angiotensin-(1–7) vs. −2.9 ± 1.3% saline] or topically [147.4 ± 18.9% angiotensin-(1–7) vs. 3.2 ± 2.8% saline] onto the UB evoked a significant (p < 0.05) increase in IP compared to saline and yielded no changes in MAP, HR, and RC. The marked response of angiotensin-(1–7) on the UB was also investigated using quantitative real-time polymerase chain reaction and western blotting assay, which demonstrated the mRNA and protein expression of Mas receptors in the bladder, respectively. ACE-2 mRNA and protein expression was also observed in the bladder. Therefore, the findings demonstrate that angiotensin-(1–7) acts in the UB to increase the IP and suggest that this peptide can be also locally synthesized in the UB.
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spelling pubmed-93454152022-08-03 Unveiling the Angiotensin-(1–7) Actions on the Urinary Bladder in Female Rats Lamy, Gustavo B. Cafarchio, Eduardo M. do Vale, Bárbara Antonio, Bruno B. Venancio, Daniel P. de Souza, Janaina S. Maciel, Rui M. Giannocco, Gisele Silva Neto, Artur F. Oyama, Lila M. Aronsson, Patrik Sato, Monica A. Front Physiol Physiology Angiotensin-(1–7) is a peptide produced by different pathways, and regardless of the route, the angiotensin-converting enzyme 2 (ACE-2) is involved in one of the steps of its synthesis. Angiotensin-(1–7) binds to Mas receptors localized in different cells throughout the body. Whether angiotensin-(1–7) exerts any action in the urinary bladder (UB) is still unknown. We investigated the effects of intravenous and topical (in situ) administration of angiotensin-(1–7) on intravesical pressure (IP) and cardiovascular variables. In addition, the Mas receptors and ACE-2 gene and protein expression were analyzed in the UB. Adult female Wistar rats were anesthetized with 2% isoflurane in 100% O(2) and submitted to the catheterization of the femoral artery and vein for mean arterial pressure (MAP) and heart rate (HR) recordings, and infusion of drugs, respectively. The renal blood flow was acquired using a Doppler flow probe placed around the left renal artery and the renal conductance (RC) was calculated as a ratio of Doppler shift (kHz) and MAP. The cannulation of the UB was performed for IP recording. We observed that angiotensin-(1–7) either administered intravenously [115.8 ± 28.6% angiotensin-(1–7) vs. −2.9 ± 1.3% saline] or topically [147.4 ± 18.9% angiotensin-(1–7) vs. 3.2 ± 2.8% saline] onto the UB evoked a significant (p < 0.05) increase in IP compared to saline and yielded no changes in MAP, HR, and RC. The marked response of angiotensin-(1–7) on the UB was also investigated using quantitative real-time polymerase chain reaction and western blotting assay, which demonstrated the mRNA and protein expression of Mas receptors in the bladder, respectively. ACE-2 mRNA and protein expression was also observed in the bladder. Therefore, the findings demonstrate that angiotensin-(1–7) acts in the UB to increase the IP and suggest that this peptide can be also locally synthesized in the UB. Frontiers Media S.A. 2022-07-19 /pmc/articles/PMC9345415/ /pubmed/35928558 http://dx.doi.org/10.3389/fphys.2022.920636 Text en Copyright © 2022 Lamy, Cafarchio, do Vale, Antonio, Venancio, de Souza, Maciel, Giannocco, Silva Neto, Oyama, Aronsson and Sato. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Lamy, Gustavo B.
Cafarchio, Eduardo M.
do Vale, Bárbara
Antonio, Bruno B.
Venancio, Daniel P.
de Souza, Janaina S.
Maciel, Rui M.
Giannocco, Gisele
Silva Neto, Artur F.
Oyama, Lila M.
Aronsson, Patrik
Sato, Monica A.
Unveiling the Angiotensin-(1–7) Actions on the Urinary Bladder in Female Rats
title Unveiling the Angiotensin-(1–7) Actions on the Urinary Bladder in Female Rats
title_full Unveiling the Angiotensin-(1–7) Actions on the Urinary Bladder in Female Rats
title_fullStr Unveiling the Angiotensin-(1–7) Actions on the Urinary Bladder in Female Rats
title_full_unstemmed Unveiling the Angiotensin-(1–7) Actions on the Urinary Bladder in Female Rats
title_short Unveiling the Angiotensin-(1–7) Actions on the Urinary Bladder in Female Rats
title_sort unveiling the angiotensin-(1–7) actions on the urinary bladder in female rats
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345415/
https://www.ncbi.nlm.nih.gov/pubmed/35928558
http://dx.doi.org/10.3389/fphys.2022.920636
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