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Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy
Oyster-derived polysaccharides (OPS) have been shown to modulate the T helper (Th)1/Th2 immunobalance toward the Th1-dominant direction in antigen-primed splenocytes. In the present study, we hypothesized that OPS might attenuate intestinal inflammation associated with food allergy, a Th2-dominant i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taiwan Food and Drug Administration
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345417/ https://www.ncbi.nlm.nih.gov/pubmed/28911394 http://dx.doi.org/10.1016/j.jfda.2015.08.006 |
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author | Cheng, Chiung-Hsiang Wu, Hsin-Ying Wu, Chi-Fang Jan, Tong-Rong |
author_facet | Cheng, Chiung-Hsiang Wu, Hsin-Ying Wu, Chi-Fang Jan, Tong-Rong |
author_sort | Cheng, Chiung-Hsiang |
collection | PubMed |
description | Oyster-derived polysaccharides (OPS) have been shown to modulate the T helper (Th)1/Th2 immunobalance toward the Th1-dominant direction in antigen-primed splenocytes. In the present study, we hypothesized that OPS might attenuate intestinal inflammation associated with food allergy, a Th2-dominant immune disorder. BALB/c mice were sensitized twice with ovalbumin (OVA) absorbed to alum and then repeatedly challenged with intragastric OVA to induce intestinal allergic responses. The mice were administered by gavage with OPS and/or vehicle (distilled water) once/d during the two sensitization phases, and once every other day during the challenge phase. Administration with OPS attenuated OVA challenge-elicited diarrhea, and the infiltration of mast cells in the intestine. OPS demonstrated a protective effect on the reduced ratio of villus length over crypt depth of the intestine in allergic mice. Furthermore, OPS administration markedly attenuated the intestinal expression of the Th2 signature cytokine interleukin-4 (IL-4). Collectively, these results demonstrated the in vivo antiallergic activity of OPS, which is associated with the suppression of allergen-induced intestinal Th2 responses and mast cell activation. |
format | Online Article Text |
id | pubmed-9345417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taiwan Food and Drug Administration |
record_format | MEDLINE/PubMed |
spelling | pubmed-93454172022-08-09 Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy Cheng, Chiung-Hsiang Wu, Hsin-Ying Wu, Chi-Fang Jan, Tong-Rong J Food Drug Anal Original Article Oyster-derived polysaccharides (OPS) have been shown to modulate the T helper (Th)1/Th2 immunobalance toward the Th1-dominant direction in antigen-primed splenocytes. In the present study, we hypothesized that OPS might attenuate intestinal inflammation associated with food allergy, a Th2-dominant immune disorder. BALB/c mice were sensitized twice with ovalbumin (OVA) absorbed to alum and then repeatedly challenged with intragastric OVA to induce intestinal allergic responses. The mice were administered by gavage with OPS and/or vehicle (distilled water) once/d during the two sensitization phases, and once every other day during the challenge phase. Administration with OPS attenuated OVA challenge-elicited diarrhea, and the infiltration of mast cells in the intestine. OPS demonstrated a protective effect on the reduced ratio of villus length over crypt depth of the intestine in allergic mice. Furthermore, OPS administration markedly attenuated the intestinal expression of the Th2 signature cytokine interleukin-4 (IL-4). Collectively, these results demonstrated the in vivo antiallergic activity of OPS, which is associated with the suppression of allergen-induced intestinal Th2 responses and mast cell activation. Taiwan Food and Drug Administration 2015-10-16 /pmc/articles/PMC9345417/ /pubmed/28911394 http://dx.doi.org/10.1016/j.jfda.2015.08.006 Text en © 2016 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Original Article Cheng, Chiung-Hsiang Wu, Hsin-Ying Wu, Chi-Fang Jan, Tong-Rong Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy |
title | Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy |
title_full | Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy |
title_fullStr | Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy |
title_full_unstemmed | Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy |
title_short | Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy |
title_sort | pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345417/ https://www.ncbi.nlm.nih.gov/pubmed/28911394 http://dx.doi.org/10.1016/j.jfda.2015.08.006 |
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