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Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography

This article presents a precolumn derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent to detect oligosaccharides and monosaccharides in Hakka rice wine. The subsequent separation of the derivatized glucose–PMP also was performed using a mobile phase consisting of the molar rat...

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Autores principales: Bai, Weidong, Fang, Xiaodi, Zhao, Wenhong, Huang, Shiqi, Zhang, Hongkang, Qian, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345461/
https://www.ncbi.nlm.nih.gov/pubmed/28911480
http://dx.doi.org/10.1016/j.jfda.2015.04.011
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author Bai, Weidong
Fang, Xiaodi
Zhao, Wenhong
Huang, Shiqi
Zhang, Hongkang
Qian, Min
author_facet Bai, Weidong
Fang, Xiaodi
Zhao, Wenhong
Huang, Shiqi
Zhang, Hongkang
Qian, Min
author_sort Bai, Weidong
collection PubMed
description This article presents a precolumn derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent to detect oligosaccharides and monosaccharides in Hakka rice wine. The subsequent separation of the derivatized glucose–PMP also was performed using a mobile phase consisting of the molar ratio of acetonitrile to ammonium acetate buffer (0.1M) of 22:78 at a flow rate of 1.0 mL/min with the column temperature of 35°C, and the pH of ammonium acetate buffer at 5.5. The optimum derivation conditions were as follows: reaction temperature, 70°C; reaction time, 30 minutes; molar ratio of PMP to glucose, 10:1 (v/v); molar ratio of sodium hydroxide to glucose, 3:1 (v/v). The recovery rates were between 93.13% and 102.08% with relative standard deviation of 0.96–2.48%. The established method provides sufficient sensitivity with values of limit of detection of 0.09–0.26 mg/L and limit of quantification of 0.27–0.87 mg/L for determination of oligosaccharides and monosaccharides.
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spelling pubmed-93454612022-08-09 Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography Bai, Weidong Fang, Xiaodi Zhao, Wenhong Huang, Shiqi Zhang, Hongkang Qian, Min J Food Drug Anal Original Article This article presents a precolumn derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent to detect oligosaccharides and monosaccharides in Hakka rice wine. The subsequent separation of the derivatized glucose–PMP also was performed using a mobile phase consisting of the molar ratio of acetonitrile to ammonium acetate buffer (0.1M) of 22:78 at a flow rate of 1.0 mL/min with the column temperature of 35°C, and the pH of ammonium acetate buffer at 5.5. The optimum derivation conditions were as follows: reaction temperature, 70°C; reaction time, 30 minutes; molar ratio of PMP to glucose, 10:1 (v/v); molar ratio of sodium hydroxide to glucose, 3:1 (v/v). The recovery rates were between 93.13% and 102.08% with relative standard deviation of 0.96–2.48%. The established method provides sufficient sensitivity with values of limit of detection of 0.09–0.26 mg/L and limit of quantification of 0.27–0.87 mg/L for determination of oligosaccharides and monosaccharides. Taiwan Food and Drug Administration 2015-07-23 /pmc/articles/PMC9345461/ /pubmed/28911480 http://dx.doi.org/10.1016/j.jfda.2015.04.011 Text en © 2015 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Bai, Weidong
Fang, Xiaodi
Zhao, Wenhong
Huang, Shiqi
Zhang, Hongkang
Qian, Min
Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography
title Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography
title_full Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography
title_fullStr Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography
title_full_unstemmed Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography
title_short Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography
title_sort determination of oligosaccharides and monosaccharides in hakka rice wine by precolumn derivation high-performance liquid chromatography
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345461/
https://www.ncbi.nlm.nih.gov/pubmed/28911480
http://dx.doi.org/10.1016/j.jfda.2015.04.011
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