Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)

We performed long-read sequencing of the Scapania undulata (L.) Dumort. chloroplast genome with Oxford Nanopore Technology using improved flow cells (10.3). S. undulata is a leafy liverwort living on rocks in or near water in mountains. The size of the complete plastome sequence is 117,651 bp long,...

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Autores principales: Ciborowski, Kamil, Skierkowski, Bartosz, Żukowska, Karolina, Krawczyk, Katarzyna, Sawicki, Jakub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Mitogenome Announcement
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347474/
https://www.ncbi.nlm.nih.gov/pubmed/35937902
http://dx.doi.org/10.1080/23802359.2022.2107458
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author Ciborowski, Kamil
Skierkowski, Bartosz
Żukowska, Karolina
Krawczyk, Katarzyna
Sawicki, Jakub
author_facet Ciborowski, Kamil
Skierkowski, Bartosz
Żukowska, Karolina
Krawczyk, Katarzyna
Sawicki, Jakub
author_sort Ciborowski, Kamil
collection PubMed
description We performed long-read sequencing of the Scapania undulata (L.) Dumort. chloroplast genome with Oxford Nanopore Technology using improved flow cells (10.3). S. undulata is a leafy liverwort living on rocks in or near water in mountains. The size of the complete plastome sequence is 117,651 bp long, the large single copy (LSC) region is 80,606 bp long, the small single copy (SSC) region is 19,401 bp long. The nanopore sequencing allowed for the distinction of inverted repeats A and B (IRA, IRB) which were not identical. Guanine cytosine (GC) ratio is 33.7% for the complete chloroplast genome, for LSC and SSC are 31.7% and 30.6%. The phylogeny reconstruction shows that Scapania undulata is the sister to S. ciliata and S. griffithii.
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spelling pubmed-93474742022-08-04 Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales) Ciborowski, Kamil Skierkowski, Bartosz Żukowska, Karolina Krawczyk, Katarzyna Sawicki, Jakub Mitochondrial DNA B Resour Mitogenome Announcement We performed long-read sequencing of the Scapania undulata (L.) Dumort. chloroplast genome with Oxford Nanopore Technology using improved flow cells (10.3). S. undulata is a leafy liverwort living on rocks in or near water in mountains. The size of the complete plastome sequence is 117,651 bp long, the large single copy (LSC) region is 80,606 bp long, the small single copy (SSC) region is 19,401 bp long. The nanopore sequencing allowed for the distinction of inverted repeats A and B (IRA, IRB) which were not identical. Guanine cytosine (GC) ratio is 33.7% for the complete chloroplast genome, for LSC and SSC are 31.7% and 30.6%. The phylogeny reconstruction shows that Scapania undulata is the sister to S. ciliata and S. griffithii. Taylor & Francis 2022-08-01 /pmc/articles/PMC9347474/ /pubmed/35937902 http://dx.doi.org/10.1080/23802359.2022.2107458 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mitogenome Announcement
Ciborowski, Kamil
Skierkowski, Bartosz
Żukowska, Karolina
Krawczyk, Katarzyna
Sawicki, Jakub
Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_full Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_fullStr Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_full_unstemmed Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_short Nanopore sequencing of chloroplast genome of Scapania undulata (L.) Dumort., 1835 (Scapaniaceae, Jungermanniales)
title_sort nanopore sequencing of chloroplast genome of scapania undulata (l.) dumort., 1835 (scapaniaceae, jungermanniales)
topic Mitogenome Announcement
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347474/
https://www.ncbi.nlm.nih.gov/pubmed/35937902
http://dx.doi.org/10.1080/23802359.2022.2107458
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