Luciferase‐based quantification of membrane fusion induced by SARS‐CoV‐2 S protein

The ongoing COVID‐19 pandemic is caused by SARS‐CoV‐2. Although several effective vaccines that target the Spike protein on the viral surface have been deployed, additional therapeutic agents are urgently needed. Here, we developed a system to measure the Spike protein function by quantifying cellul...

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Autores principales: Haga, Kei, Takai‐Todaka, Reiko, Sawada, Akihito, Katayama, Kazuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347681/
https://www.ncbi.nlm.nih.gov/pubmed/35488742
http://dx.doi.org/10.1111/gtc.12945
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author Haga, Kei
Takai‐Todaka, Reiko
Sawada, Akihito
Katayama, Kazuhiko
author_facet Haga, Kei
Takai‐Todaka, Reiko
Sawada, Akihito
Katayama, Kazuhiko
author_sort Haga, Kei
collection PubMed
description The ongoing COVID‐19 pandemic is caused by SARS‐CoV‐2. Although several effective vaccines that target the Spike protein on the viral surface have been deployed, additional therapeutic agents are urgently needed. Here, we developed a system to measure the Spike protein function by quantifying cellular membrane fusion induced by the Spike protein. The system enables the evaluation of the effects of drugs and neutralizing antibodies against SARS‐CoV‐2 without using live viruses. Furthermore, the system characterizes membrane fusion activity of the Spike protein of each variant to reveal that Delta variant has more potent than Wuhan and Omicron. Our system could lead to develop high‐throughput screening for drug candidates and neutralization antibodies that target virus entry and characterize Spike proteins from variants.
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spelling pubmed-93476812022-08-03 Luciferase‐based quantification of membrane fusion induced by SARS‐CoV‐2 S protein Haga, Kei Takai‐Todaka, Reiko Sawada, Akihito Katayama, Kazuhiko Genes Cells Brief Reports The ongoing COVID‐19 pandemic is caused by SARS‐CoV‐2. Although several effective vaccines that target the Spike protein on the viral surface have been deployed, additional therapeutic agents are urgently needed. Here, we developed a system to measure the Spike protein function by quantifying cellular membrane fusion induced by the Spike protein. The system enables the evaluation of the effects of drugs and neutralizing antibodies against SARS‐CoV‐2 without using live viruses. Furthermore, the system characterizes membrane fusion activity of the Spike protein of each variant to reveal that Delta variant has more potent than Wuhan and Omicron. Our system could lead to develop high‐throughput screening for drug candidates and neutralization antibodies that target virus entry and characterize Spike proteins from variants. John Wiley and Sons Inc. 2022-05-17 /pmc/articles/PMC9347681/ /pubmed/35488742 http://dx.doi.org/10.1111/gtc.12945 Text en © 2022 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Brief Reports
Haga, Kei
Takai‐Todaka, Reiko
Sawada, Akihito
Katayama, Kazuhiko
Luciferase‐based quantification of membrane fusion induced by SARS‐CoV‐2 S protein
title Luciferase‐based quantification of membrane fusion induced by SARS‐CoV‐2 S protein
title_full Luciferase‐based quantification of membrane fusion induced by SARS‐CoV‐2 S protein
title_fullStr Luciferase‐based quantification of membrane fusion induced by SARS‐CoV‐2 S protein
title_full_unstemmed Luciferase‐based quantification of membrane fusion induced by SARS‐CoV‐2 S protein
title_short Luciferase‐based quantification of membrane fusion induced by SARS‐CoV‐2 S protein
title_sort luciferase‐based quantification of membrane fusion induced by sars‐cov‐2 s protein
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347681/
https://www.ncbi.nlm.nih.gov/pubmed/35488742
http://dx.doi.org/10.1111/gtc.12945
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