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COVID salivary diagnostics: A comparative technical study

Since the beginning of the coronavirus disease 2019 (COVID‐19) pandemic, molecular diagnostics of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) have taken center stage in the detection of infected individuals for isolation purposes but also in the mass surveillance as a preventive str...

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Autores principales: Nguyen‐Kim, Hanh, Beckmann, Christiane, Redondo, Maurice, Ziliox, Jérémy, Vallet, Virginie, Berger‐Sturm, Karin, Overbeck, Jan Von, Alberi Auber, Lavinia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347777/
https://www.ncbi.nlm.nih.gov/pubmed/35614569
http://dx.doi.org/10.1002/jmv.27883
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author Nguyen‐Kim, Hanh
Beckmann, Christiane
Redondo, Maurice
Ziliox, Jérémy
Vallet, Virginie
Berger‐Sturm, Karin
Overbeck, Jan Von
Alberi Auber, Lavinia
author_facet Nguyen‐Kim, Hanh
Beckmann, Christiane
Redondo, Maurice
Ziliox, Jérémy
Vallet, Virginie
Berger‐Sturm, Karin
Overbeck, Jan Von
Alberi Auber, Lavinia
author_sort Nguyen‐Kim, Hanh
collection PubMed
description Since the beginning of the coronavirus disease 2019 (COVID‐19) pandemic, molecular diagnostics of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) have taken center stage in the detection of infected individuals for isolation purposes but also in the mass surveillance as a preventive strategy to contain the virus spread. While nasopharyngeal swabs (NPS) have remained the golden standard substrate, salivary diagnostic for SARS‐CoV‐2 has been proposed as an alternative and noninvasive measure in vulnerable individuals. Nevertheless, there is a widespread assumption that salivary reverse‐transcription polymerase chain reaction (RT‐PCR) does not match the quality of testing using NPS and particular care should be taken in respect to food or beverage intake, when sampling saliva. Our study indicates that without any precaution in the selection of 190 patients, nor restriction over the time window of sampling, there is 99% match in the COVID‐19 positivity between NPS and saliva when using RT‐PCR, with a reported Delta in thermal cycles (Cts) values for the viral genes Envelope (E) and Open reading frame 1ab (Orf1ab) between 0 and 2, a 98.7% sensitivity and 100% specificity. This high accuracy is maintained in pooling configurations that can be used for mass‐testing purposes in professional and educational settings. The further advantage to using crude saliva as compared to NPS or mouthwash is that direct methods yield robust results. Overall, our study validates and promotes the use of salivary diagnostic for COVID‐19 eliminating the need of a medical practitioner for the sampling, resolving the unpleasantness of the NPS intervention and empowering the patient to do self‐testing in times of need.
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spelling pubmed-93477772022-08-04 COVID salivary diagnostics: A comparative technical study Nguyen‐Kim, Hanh Beckmann, Christiane Redondo, Maurice Ziliox, Jérémy Vallet, Virginie Berger‐Sturm, Karin Overbeck, Jan Von Alberi Auber, Lavinia J Med Virol Research Articles Since the beginning of the coronavirus disease 2019 (COVID‐19) pandemic, molecular diagnostics of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) have taken center stage in the detection of infected individuals for isolation purposes but also in the mass surveillance as a preventive strategy to contain the virus spread. While nasopharyngeal swabs (NPS) have remained the golden standard substrate, salivary diagnostic for SARS‐CoV‐2 has been proposed as an alternative and noninvasive measure in vulnerable individuals. Nevertheless, there is a widespread assumption that salivary reverse‐transcription polymerase chain reaction (RT‐PCR) does not match the quality of testing using NPS and particular care should be taken in respect to food or beverage intake, when sampling saliva. Our study indicates that without any precaution in the selection of 190 patients, nor restriction over the time window of sampling, there is 99% match in the COVID‐19 positivity between NPS and saliva when using RT‐PCR, with a reported Delta in thermal cycles (Cts) values for the viral genes Envelope (E) and Open reading frame 1ab (Orf1ab) between 0 and 2, a 98.7% sensitivity and 100% specificity. This high accuracy is maintained in pooling configurations that can be used for mass‐testing purposes in professional and educational settings. The further advantage to using crude saliva as compared to NPS or mouthwash is that direct methods yield robust results. Overall, our study validates and promotes the use of salivary diagnostic for COVID‐19 eliminating the need of a medical practitioner for the sampling, resolving the unpleasantness of the NPS intervention and empowering the patient to do self‐testing in times of need. John Wiley and Sons Inc. 2022-06-02 2022-09 /pmc/articles/PMC9347777/ /pubmed/35614569 http://dx.doi.org/10.1002/jmv.27883 Text en © 2022 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Nguyen‐Kim, Hanh
Beckmann, Christiane
Redondo, Maurice
Ziliox, Jérémy
Vallet, Virginie
Berger‐Sturm, Karin
Overbeck, Jan Von
Alberi Auber, Lavinia
COVID salivary diagnostics: A comparative technical study
title COVID salivary diagnostics: A comparative technical study
title_full COVID salivary diagnostics: A comparative technical study
title_fullStr COVID salivary diagnostics: A comparative technical study
title_full_unstemmed COVID salivary diagnostics: A comparative technical study
title_short COVID salivary diagnostics: A comparative technical study
title_sort covid salivary diagnostics: a comparative technical study
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347777/
https://www.ncbi.nlm.nih.gov/pubmed/35614569
http://dx.doi.org/10.1002/jmv.27883
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