Cargando…

Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS‐CoV‐2 variants

A prospective cohort study was conducted during the Delta and Omicron severe acute respiratory syndrome coronavirus type 2 (SARS‐CoV‐2) epidemic waves from paired nasopharyngeal swab (NPS or NP swab) and saliva samples taken from 624 participants. The study aimed to assess if any differences among p...

Descripción completa

Detalles Bibliográficos
Autores principales: Uršič, Tina, Kogoj, Rok, Šikonja, Jaka, Roškarič, Damijana, Jevšnik Virant, Monika, Bogovič, Petra, Petrovec, Miroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348014/
https://www.ncbi.nlm.nih.gov/pubmed/35642439
http://dx.doi.org/10.1002/jmv.27898
_version_ 1784761895272579072
author Uršič, Tina
Kogoj, Rok
Šikonja, Jaka
Roškarič, Damijana
Jevšnik Virant, Monika
Bogovič, Petra
Petrovec, Miroslav
author_facet Uršič, Tina
Kogoj, Rok
Šikonja, Jaka
Roškarič, Damijana
Jevšnik Virant, Monika
Bogovič, Petra
Petrovec, Miroslav
author_sort Uršič, Tina
collection PubMed
description A prospective cohort study was conducted during the Delta and Omicron severe acute respiratory syndrome coronavirus type 2 (SARS‐CoV‐2) epidemic waves from paired nasopharyngeal swab (NPS or NP swab) and saliva samples taken from 624 participants. The study aimed to assess if any differences among participants from both waves could be observed and if any difference in molecular diagnostic performance could be observed among the two sample types. Samples were transported immediately to the laboratory to ensure the highest possible sample quality without any freezing and thawing steps before processing. Nucleic acids from saliva and NPS were prospectively extracted and SARS‐CoV‐2 was detected using a real‐time reverse‐transcription polymerase chain reaction. All observed results were statistically analyzed. Although the results obtained with NP and saliva agreed overall, higher viral loads were observed in NP swabs regardless of the day of specimen collection in both SARS‐CoV‐2 epidemic waves. No significant difference could be observed between the two epidemic waves characterized by Delta or Omicron SARS‐CoV‐2. To note, Delta infection resulted in higher viral loads both in NP and saliva and more symptoms, including rhinorrhea, cough, and dyspnea, whereas Omicron wave patients more frequently reported sore throat. An increase in the mean log RNA of SARS‐CoV‐2 was observed with the number of expressed symptoms in both waves, however, the difference was not significant. Data confirmed that results from saliva were concordant with those from NP swabs, although saliva proved to be a challenging sample with frequent inhibitions that required substantial retesting.
format Online
Article
Text
id pubmed-9348014
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-93480142022-08-04 Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS‐CoV‐2 variants Uršič, Tina Kogoj, Rok Šikonja, Jaka Roškarič, Damijana Jevšnik Virant, Monika Bogovič, Petra Petrovec, Miroslav J Med Virol Research Articles A prospective cohort study was conducted during the Delta and Omicron severe acute respiratory syndrome coronavirus type 2 (SARS‐CoV‐2) epidemic waves from paired nasopharyngeal swab (NPS or NP swab) and saliva samples taken from 624 participants. The study aimed to assess if any differences among participants from both waves could be observed and if any difference in molecular diagnostic performance could be observed among the two sample types. Samples were transported immediately to the laboratory to ensure the highest possible sample quality without any freezing and thawing steps before processing. Nucleic acids from saliva and NPS were prospectively extracted and SARS‐CoV‐2 was detected using a real‐time reverse‐transcription polymerase chain reaction. All observed results were statistically analyzed. Although the results obtained with NP and saliva agreed overall, higher viral loads were observed in NP swabs regardless of the day of specimen collection in both SARS‐CoV‐2 epidemic waves. No significant difference could be observed between the two epidemic waves characterized by Delta or Omicron SARS‐CoV‐2. To note, Delta infection resulted in higher viral loads both in NP and saliva and more symptoms, including rhinorrhea, cough, and dyspnea, whereas Omicron wave patients more frequently reported sore throat. An increase in the mean log RNA of SARS‐CoV‐2 was observed with the number of expressed symptoms in both waves, however, the difference was not significant. Data confirmed that results from saliva were concordant with those from NP swabs, although saliva proved to be a challenging sample with frequent inhibitions that required substantial retesting. John Wiley and Sons Inc. 2022-06-08 2022-10 /pmc/articles/PMC9348014/ /pubmed/35642439 http://dx.doi.org/10.1002/jmv.27898 Text en © 2022 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Uršič, Tina
Kogoj, Rok
Šikonja, Jaka
Roškarič, Damijana
Jevšnik Virant, Monika
Bogovič, Petra
Petrovec, Miroslav
Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS‐CoV‐2 variants
title Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS‐CoV‐2 variants
title_full Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS‐CoV‐2 variants
title_fullStr Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS‐CoV‐2 variants
title_full_unstemmed Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS‐CoV‐2 variants
title_short Performance of nasopharyngeal swab and saliva in detecting Delta and Omicron SARS‐CoV‐2 variants
title_sort performance of nasopharyngeal swab and saliva in detecting delta and omicron sars‐cov‐2 variants
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348014/
https://www.ncbi.nlm.nih.gov/pubmed/35642439
http://dx.doi.org/10.1002/jmv.27898
work_keys_str_mv AT ursictina performanceofnasopharyngealswabandsalivaindetectingdeltaandomicronsarscov2variants
AT kogojrok performanceofnasopharyngealswabandsalivaindetectingdeltaandomicronsarscov2variants
AT sikonjajaka performanceofnasopharyngealswabandsalivaindetectingdeltaandomicronsarscov2variants
AT roskaricdamijana performanceofnasopharyngealswabandsalivaindetectingdeltaandomicronsarscov2variants
AT jevsnikvirantmonika performanceofnasopharyngealswabandsalivaindetectingdeltaandomicronsarscov2variants
AT bogovicpetra performanceofnasopharyngealswabandsalivaindetectingdeltaandomicronsarscov2variants
AT petrovecmiroslav performanceofnasopharyngealswabandsalivaindetectingdeltaandomicronsarscov2variants