Cargando…

Effect of Cassia fistula L. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat

BACKGROUND: Cassia fistula was used traditionally as laxative in pregnant women. Nevertheless, its fetal and maternal effects in pregnancy have not been studied yet. METHODS: Oral (Lethal Dose, 50%) LD50 was determined in mice. In addition, a control group, pregnant rats in other 5 experimental grou...

Descripción completa

Detalles Bibliográficos
Autores principales: Hakiminia, Saeed, Esmaeeli, Zahra, Moghadamnia, Ali Akbar, Jorsaraei, Seyyed Gholam Ali, Feizi, Farideh, Khafri, Sorayya, Memariani, Zahra, Shirafkan, Hoda, Mozaffarpur, Seyyed Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348213/
https://www.ncbi.nlm.nih.gov/pubmed/35974950
http://dx.doi.org/10.22088/cjim.13.3.475
_version_ 1784761912320327680
author Hakiminia, Saeed
Esmaeeli, Zahra
Moghadamnia, Ali Akbar
Jorsaraei, Seyyed Gholam Ali
Feizi, Farideh
Khafri, Sorayya
Memariani, Zahra
Shirafkan, Hoda
Mozaffarpur, Seyyed Ali
author_facet Hakiminia, Saeed
Esmaeeli, Zahra
Moghadamnia, Ali Akbar
Jorsaraei, Seyyed Gholam Ali
Feizi, Farideh
Khafri, Sorayya
Memariani, Zahra
Shirafkan, Hoda
Mozaffarpur, Seyyed Ali
author_sort Hakiminia, Saeed
collection PubMed
description BACKGROUND: Cassia fistula was used traditionally as laxative in pregnant women. Nevertheless, its fetal and maternal effects in pregnancy have not been studied yet. METHODS: Oral (Lethal Dose, 50%) LD50 was determined in mice. In addition, a control group, pregnant rats in other 5 experimental groups (n=12) received orally C. fistula aqueous extract (500, 1000 and 2000 mg/kg), tween80 (10%) and distilled water during pregnancy up to the delivery (21-23 days). Some serum indices were evaluated in maternal blood samples after delivery. Histopathologic and histomorphometric evaluations were performed on the selected slices of newborn rats. RESULTS: Anthraquinone‎ content of the aqueous extract was 0.34% w/w. Oral LD50 was obtained more than 5000mg/kg. No abortions and newborn anomalies were observed in groups. The height and weight of the offspring were significantly reduced by the administration of 500, and 2000 mg/kg of extract compared to control. There was no significant change in maternal blood urea and creatinine. Higher concentration (2000mg/kg) led to ALT elevation. ALS levels decreased dose-dependency in treatment groups comparing to control. Histopathological findings showed significant lung vascular congestion, and hypertrophy of heart in group tween80, and significant hepatic parenchymal inflammation in tween80 and 2000mg/kg and 1000mg/kg groups. In all tissues of all groups, malpighian body area and bowman’s capsule space significantly increased compared to the control group. CONCLUSION: It seems C. fistula extract is safe in pregnancy. Because of confounding role of tween80 in histopathological finding, more research is necessary.
format Online
Article
Text
id pubmed-9348213
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Babol University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-93482132022-08-15 Effect of Cassia fistula L. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat Hakiminia, Saeed Esmaeeli, Zahra Moghadamnia, Ali Akbar Jorsaraei, Seyyed Gholam Ali Feizi, Farideh Khafri, Sorayya Memariani, Zahra Shirafkan, Hoda Mozaffarpur, Seyyed Ali Caspian J Intern Med Original Article BACKGROUND: Cassia fistula was used traditionally as laxative in pregnant women. Nevertheless, its fetal and maternal effects in pregnancy have not been studied yet. METHODS: Oral (Lethal Dose, 50%) LD50 was determined in mice. In addition, a control group, pregnant rats in other 5 experimental groups (n=12) received orally C. fistula aqueous extract (500, 1000 and 2000 mg/kg), tween80 (10%) and distilled water during pregnancy up to the delivery (21-23 days). Some serum indices were evaluated in maternal blood samples after delivery. Histopathologic and histomorphometric evaluations were performed on the selected slices of newborn rats. RESULTS: Anthraquinone‎ content of the aqueous extract was 0.34% w/w. Oral LD50 was obtained more than 5000mg/kg. No abortions and newborn anomalies were observed in groups. The height and weight of the offspring were significantly reduced by the administration of 500, and 2000 mg/kg of extract compared to control. There was no significant change in maternal blood urea and creatinine. Higher concentration (2000mg/kg) led to ALT elevation. ALS levels decreased dose-dependency in treatment groups comparing to control. Histopathological findings showed significant lung vascular congestion, and hypertrophy of heart in group tween80, and significant hepatic parenchymal inflammation in tween80 and 2000mg/kg and 1000mg/kg groups. In all tissues of all groups, malpighian body area and bowman’s capsule space significantly increased compared to the control group. CONCLUSION: It seems C. fistula extract is safe in pregnancy. Because of confounding role of tween80 in histopathological finding, more research is necessary. Babol University of Medical Sciences 2022 /pmc/articles/PMC9348213/ /pubmed/35974950 http://dx.doi.org/10.22088/cjim.13.3.475 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hakiminia, Saeed
Esmaeeli, Zahra
Moghadamnia, Ali Akbar
Jorsaraei, Seyyed Gholam Ali
Feizi, Farideh
Khafri, Sorayya
Memariani, Zahra
Shirafkan, Hoda
Mozaffarpur, Seyyed Ali
Effect of Cassia fistula L. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat
title Effect of Cassia fistula L. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat
title_full Effect of Cassia fistula L. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat
title_fullStr Effect of Cassia fistula L. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat
title_full_unstemmed Effect of Cassia fistula L. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat
title_short Effect of Cassia fistula L. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat
title_sort effect of cassia fistula l. aqueous extract in maternal reproductive outcome, some serum indices and fetal anomaly frequency in rat
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348213/
https://www.ncbi.nlm.nih.gov/pubmed/35974950
http://dx.doi.org/10.22088/cjim.13.3.475
work_keys_str_mv AT hakiminiasaeed effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat
AT esmaeelizahra effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat
AT moghadamniaaliakbar effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat
AT jorsaraeiseyyedgholamali effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat
AT feizifarideh effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat
AT khafrisorayya effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat
AT memarianizahra effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat
AT shirafkanhoda effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat
AT mozaffarpurseyyedali effectofcassiafistulalaqueousextractinmaternalreproductiveoutcomesomeserumindicesandfetalanomalyfrequencyinrat