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Parallel study of transient dosing of antibiotics in a microfluidic device
Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348895/ https://www.ncbi.nlm.nih.gov/pubmed/35935120 http://dx.doi.org/10.1063/5.0091704 |
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author | Rackus, Darius G. Jusková, Petra Yokoyama, Fumiaki Dittrich, Petra S. |
author_facet | Rackus, Darius G. Jusková, Petra Yokoyama, Fumiaki Dittrich, Petra S. |
author_sort | Rackus, Darius G. |
collection | PubMed |
description | Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping and cultivating bacteria in hydrogel plugs. Ring-shaped isolation valves are used to define the shape of the plugs and also to control exposure of the plugs to the surrounding medium. We demonstrate bacterial cultivation, determination of the minimum inhibitory concentration of an antibiotic, and transient dosing of an antibiotic at sub-1-h doses. The transient dosing experiments reveal that at dose durations on the order of minutes, ampicillin's bactericidal effect has both a time and concentration dependency. |
format | Online Article Text |
id | pubmed-9348895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-93488952023-02-22 Parallel study of transient dosing of antibiotics in a microfluidic device Rackus, Darius G. Jusková, Petra Yokoyama, Fumiaki Dittrich, Petra S. Biomicrofluidics Regular Articles Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping and cultivating bacteria in hydrogel plugs. Ring-shaped isolation valves are used to define the shape of the plugs and also to control exposure of the plugs to the surrounding medium. We demonstrate bacterial cultivation, determination of the minimum inhibitory concentration of an antibiotic, and transient dosing of an antibiotic at sub-1-h doses. The transient dosing experiments reveal that at dose durations on the order of minutes, ampicillin's bactericidal effect has both a time and concentration dependency. AIP Publishing LLC 2022-08-01 /pmc/articles/PMC9348895/ /pubmed/35935120 http://dx.doi.org/10.1063/5.0091704 Text en © 2022 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). Published open access through an agreement with Eidgenossische Technische Hochschule Zurich Department of Biosystems Science and Engineering211122 |
spellingShingle | Regular Articles Rackus, Darius G. Jusková, Petra Yokoyama, Fumiaki Dittrich, Petra S. Parallel study of transient dosing of antibiotics in a microfluidic device |
title | Parallel study of transient dosing of antibiotics in a microfluidic device |
title_full | Parallel study of transient dosing of antibiotics in a microfluidic device |
title_fullStr | Parallel study of transient dosing of antibiotics in a microfluidic device |
title_full_unstemmed | Parallel study of transient dosing of antibiotics in a microfluidic device |
title_short | Parallel study of transient dosing of antibiotics in a microfluidic device |
title_sort | parallel study of transient dosing of antibiotics in a microfluidic device |
topic | Regular Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348895/ https://www.ncbi.nlm.nih.gov/pubmed/35935120 http://dx.doi.org/10.1063/5.0091704 |
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