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Parallel study of transient dosing of antibiotics in a microfluidic device

Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping...

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Autores principales: Rackus, Darius G., Jusková, Petra, Yokoyama, Fumiaki, Dittrich, Petra S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348895/
https://www.ncbi.nlm.nih.gov/pubmed/35935120
http://dx.doi.org/10.1063/5.0091704
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author Rackus, Darius G.
Jusková, Petra
Yokoyama, Fumiaki
Dittrich, Petra S.
author_facet Rackus, Darius G.
Jusková, Petra
Yokoyama, Fumiaki
Dittrich, Petra S.
author_sort Rackus, Darius G.
collection PubMed
description Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping and cultivating bacteria in hydrogel plugs. Ring-shaped isolation valves are used to define the shape of the plugs and also to control exposure of the plugs to the surrounding medium. We demonstrate bacterial cultivation, determination of the minimum inhibitory concentration of an antibiotic, and transient dosing of an antibiotic at sub-1-h doses. The transient dosing experiments reveal that at dose durations on the order of minutes, ampicillin's bactericidal effect has both a time and concentration dependency.
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spelling pubmed-93488952023-02-22 Parallel study of transient dosing of antibiotics in a microfluidic device Rackus, Darius G. Jusková, Petra Yokoyama, Fumiaki Dittrich, Petra S. Biomicrofluidics Regular Articles Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping and cultivating bacteria in hydrogel plugs. Ring-shaped isolation valves are used to define the shape of the plugs and also to control exposure of the plugs to the surrounding medium. We demonstrate bacterial cultivation, determination of the minimum inhibitory concentration of an antibiotic, and transient dosing of an antibiotic at sub-1-h doses. The transient dosing experiments reveal that at dose durations on the order of minutes, ampicillin's bactericidal effect has both a time and concentration dependency. AIP Publishing LLC 2022-08-01 /pmc/articles/PMC9348895/ /pubmed/35935120 http://dx.doi.org/10.1063/5.0091704 Text en © 2022 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). Published open access through an agreement with Eidgenossische Technische Hochschule Zurich Department of Biosystems Science and Engineering211122
spellingShingle Regular Articles
Rackus, Darius G.
Jusková, Petra
Yokoyama, Fumiaki
Dittrich, Petra S.
Parallel study of transient dosing of antibiotics in a microfluidic device
title Parallel study of transient dosing of antibiotics in a microfluidic device
title_full Parallel study of transient dosing of antibiotics in a microfluidic device
title_fullStr Parallel study of transient dosing of antibiotics in a microfluidic device
title_full_unstemmed Parallel study of transient dosing of antibiotics in a microfluidic device
title_short Parallel study of transient dosing of antibiotics in a microfluidic device
title_sort parallel study of transient dosing of antibiotics in a microfluidic device
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348895/
https://www.ncbi.nlm.nih.gov/pubmed/35935120
http://dx.doi.org/10.1063/5.0091704
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