Cargando…
Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles
Lava caves, tubes, and fumaroles in Hawai‘i present a range of volcanic, oligotrophic environments from different lava flows and host unexpectedly high levels of bacterial diversity. These features provide an opportunity to study the ecological drivers that structure bacterial community diversity an...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349362/ https://www.ncbi.nlm.nih.gov/pubmed/35935195 http://dx.doi.org/10.3389/fmicb.2022.934708 |
_version_ | 1784762114955542528 |
---|---|
author | Prescott, Rebecca D. Zamkovaya, Tatyana Donachie, Stuart P. Northup, Diana E. Medley, Joseph J. Monsalve, Natalia Saw, Jimmy H. Decho, Alan W. Chain, Patrick S. G. Boston, Penelope J. |
author_facet | Prescott, Rebecca D. Zamkovaya, Tatyana Donachie, Stuart P. Northup, Diana E. Medley, Joseph J. Monsalve, Natalia Saw, Jimmy H. Decho, Alan W. Chain, Patrick S. G. Boston, Penelope J. |
author_sort | Prescott, Rebecca D. |
collection | PubMed |
description | Lava caves, tubes, and fumaroles in Hawai‘i present a range of volcanic, oligotrophic environments from different lava flows and host unexpectedly high levels of bacterial diversity. These features provide an opportunity to study the ecological drivers that structure bacterial community diversity and assemblies in volcanic ecosystems and compare the older, more stable environments of lava tubes, to the more variable and extreme conditions of younger, geothermally active caves and fumaroles. Using 16S rRNA amplicon-based sequencing methods, we investigated the phylogenetic distinctness and diversity and identified microbial interactions and consortia through co-occurrence networks in 70 samples from lava tubes, geothermal lava caves, and fumaroles on the island of Hawai‘i. Our data illustrate that lava caves and geothermal sites harbor unique microbial communities, with very little overlap between caves or sites. We also found that older lava tubes (500–800 yrs old) hosted greater phylogenetic diversity (Faith's PD) than sites that were either geothermally active or younger (<400 yrs old). Geothermally active sites had a greater number of interactions and complexity than lava tubes. Average phylogenetic distinctness, a measure of the phylogenetic relatedness of a community, was higher than would be expected if communities were structured at random. This suggests that bacterial communities of Hawaiian volcanic environments are phylogenetically over-dispersed and that competitive exclusion is the main driver in structuring these communities. This was supported by network analyses that found that taxa (Class level) co-occurred with more distantly related organisms than close relatives, particularly in geothermal sites. Network “hubs” (taxa of potentially higher ecological importance) were not the most abundant taxa in either geothermal sites or lava tubes and were identified as unknown families or genera of the phyla, Chloroflexi and Acidobacteria. These results highlight the need for further study on the ecological role of microbes in caves through targeted culturing methods, metagenomics, and long-read sequence technologies. |
format | Online Article Text |
id | pubmed-9349362 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93493622022-08-05 Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles Prescott, Rebecca D. Zamkovaya, Tatyana Donachie, Stuart P. Northup, Diana E. Medley, Joseph J. Monsalve, Natalia Saw, Jimmy H. Decho, Alan W. Chain, Patrick S. G. Boston, Penelope J. Front Microbiol Microbiology Lava caves, tubes, and fumaroles in Hawai‘i present a range of volcanic, oligotrophic environments from different lava flows and host unexpectedly high levels of bacterial diversity. These features provide an opportunity to study the ecological drivers that structure bacterial community diversity and assemblies in volcanic ecosystems and compare the older, more stable environments of lava tubes, to the more variable and extreme conditions of younger, geothermally active caves and fumaroles. Using 16S rRNA amplicon-based sequencing methods, we investigated the phylogenetic distinctness and diversity and identified microbial interactions and consortia through co-occurrence networks in 70 samples from lava tubes, geothermal lava caves, and fumaroles on the island of Hawai‘i. Our data illustrate that lava caves and geothermal sites harbor unique microbial communities, with very little overlap between caves or sites. We also found that older lava tubes (500–800 yrs old) hosted greater phylogenetic diversity (Faith's PD) than sites that were either geothermally active or younger (<400 yrs old). Geothermally active sites had a greater number of interactions and complexity than lava tubes. Average phylogenetic distinctness, a measure of the phylogenetic relatedness of a community, was higher than would be expected if communities were structured at random. This suggests that bacterial communities of Hawaiian volcanic environments are phylogenetically over-dispersed and that competitive exclusion is the main driver in structuring these communities. This was supported by network analyses that found that taxa (Class level) co-occurred with more distantly related organisms than close relatives, particularly in geothermal sites. Network “hubs” (taxa of potentially higher ecological importance) were not the most abundant taxa in either geothermal sites or lava tubes and were identified as unknown families or genera of the phyla, Chloroflexi and Acidobacteria. These results highlight the need for further study on the ecological role of microbes in caves through targeted culturing methods, metagenomics, and long-read sequence technologies. Frontiers Media S.A. 2022-07-21 /pmc/articles/PMC9349362/ /pubmed/35935195 http://dx.doi.org/10.3389/fmicb.2022.934708 Text en Copyright © 2022 Prescott, Zamkovaya, Donachie, Northup, Medley, Monsalve, Saw, Decho, Chain and Boston. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Prescott, Rebecca D. Zamkovaya, Tatyana Donachie, Stuart P. Northup, Diana E. Medley, Joseph J. Monsalve, Natalia Saw, Jimmy H. Decho, Alan W. Chain, Patrick S. G. Boston, Penelope J. Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles |
title | Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles |
title_full | Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles |
title_fullStr | Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles |
title_full_unstemmed | Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles |
title_short | Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles |
title_sort | islands within islands: bacterial phylogenetic structure and consortia in hawaiian lava caves and fumaroles |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9349362/ https://www.ncbi.nlm.nih.gov/pubmed/35935195 http://dx.doi.org/10.3389/fmicb.2022.934708 |
work_keys_str_mv | AT prescottrebeccad islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT zamkovayatatyana islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT donachiestuartp islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT northupdianae islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT medleyjosephj islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT monsalvenatalia islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT sawjimmyh islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT dechoalanw islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT chainpatricksg islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles AT bostonpenelopej islandswithinislandsbacterialphylogeneticstructureandconsortiainhawaiianlavacavesandfumaroles |