Pulse dipolar EPR for determining nanomolar binding affinities

Protein interaction studies often require very low concentrations and highly sensitive biophysical methods. Here, we demonstrate that pulse dipolar electron paramagnetic resonance spectroscopy allows measuring dissociation constants in the nanomolar range. This approach is appealing for concentratio...

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Detalles Bibliográficos
Autores principales: Ackermann, Katrin, Wort, Joshua L., Bode, Bela E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350988/
https://www.ncbi.nlm.nih.gov/pubmed/35837993
http://dx.doi.org/10.1039/d2cc02360a
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author Ackermann, Katrin
Wort, Joshua L.
Bode, Bela E.
author_facet Ackermann, Katrin
Wort, Joshua L.
Bode, Bela E.
author_sort Ackermann, Katrin
collection PubMed
description Protein interaction studies often require very low concentrations and highly sensitive biophysical methods. Here, we demonstrate that pulse dipolar electron paramagnetic resonance spectroscopy allows measuring dissociation constants in the nanomolar range. This approach is appealing for concentration-limited biomolecular systems and medium-to-high-affinity binding studies, demonstrated here at 50 nanomolar protein concentration.
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spelling pubmed-93509882022-08-29 Pulse dipolar EPR for determining nanomolar binding affinities Ackermann, Katrin Wort, Joshua L. Bode, Bela E. Chem Commun (Camb) Chemistry Protein interaction studies often require very low concentrations and highly sensitive biophysical methods. Here, we demonstrate that pulse dipolar electron paramagnetic resonance spectroscopy allows measuring dissociation constants in the nanomolar range. This approach is appealing for concentration-limited biomolecular systems and medium-to-high-affinity binding studies, demonstrated here at 50 nanomolar protein concentration. The Royal Society of Chemistry 2022-07-11 /pmc/articles/PMC9350988/ /pubmed/35837993 http://dx.doi.org/10.1039/d2cc02360a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Ackermann, Katrin
Wort, Joshua L.
Bode, Bela E.
Pulse dipolar EPR for determining nanomolar binding affinities
title Pulse dipolar EPR for determining nanomolar binding affinities
title_full Pulse dipolar EPR for determining nanomolar binding affinities
title_fullStr Pulse dipolar EPR for determining nanomolar binding affinities
title_full_unstemmed Pulse dipolar EPR for determining nanomolar binding affinities
title_short Pulse dipolar EPR for determining nanomolar binding affinities
title_sort pulse dipolar epr for determining nanomolar binding affinities
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350988/
https://www.ncbi.nlm.nih.gov/pubmed/35837993
http://dx.doi.org/10.1039/d2cc02360a
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