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Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract
Hypericum perforatum L. (Hypericaceae) has been widely prescribed for mild to moderate depression following the release of promising results in clinical trials. However, it is known that its constituents may be affected by milieu. The stability complexities of the constituents of H. perforatum have...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taiwan Food and Drug Administration
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351782/ https://www.ncbi.nlm.nih.gov/pubmed/28911714 http://dx.doi.org/10.1016/j.jfda.2015.04.002 |
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author | Koyu, Halil Haznedaroglu, Mehmet Zeki |
author_facet | Koyu, Halil Haznedaroglu, Mehmet Zeki |
author_sort | Koyu, Halil |
collection | PubMed |
description | Hypericum perforatum L. (Hypericaceae) has been widely prescribed for mild to moderate depression following the release of promising results in clinical trials. However, it is known that its constituents may be affected by milieu. The stability complexities of the constituents of H. perforatum have gained interest in recent years. The aim of the present study was to examine the impact of storage conditions on H. perforatum total extract simultaneously under different storage conditions. Temperature, humidity, and light conditions were evaluated. Comparative analyses of methanol extracts were conducted using high performance liquid chromatographydiode array detection for chlorogenic acid, rutin, hyperoside, isoquercitrin, quercitrin, quercetin, amentoflavone, pseudohypericin, hyperforin, and hypericin. Analysis and extraction were performed using a validated method. The fluctuation of the constituents of the plant extract has been demonstrated. Among these components, chlorogenic acid was the most stable. Hyperforin, hypericin, and pseudohypericin were more stable than the flavonoids at −20°C, in the 6(th) month. As estimated, decay was lowest at −20°C and highest at 40°C–75% relative humidity for the analyzed constituents. Except for hyperforin, light protection decreased the breakdown of components within 4 months. However, at the 6(th) month, equivalent changes were seen for all constituents. Degradation of the constituents at −20°C indicates the importance of stability tests in analysis studies covering time and storage conditions. |
format | Online Article Text |
id | pubmed-9351782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taiwan Food and Drug Administration |
record_format | MEDLINE/PubMed |
spelling | pubmed-93517822022-08-09 Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract Koyu, Halil Haznedaroglu, Mehmet Zeki J Food Drug Anal Original Article Hypericum perforatum L. (Hypericaceae) has been widely prescribed for mild to moderate depression following the release of promising results in clinical trials. However, it is known that its constituents may be affected by milieu. The stability complexities of the constituents of H. perforatum have gained interest in recent years. The aim of the present study was to examine the impact of storage conditions on H. perforatum total extract simultaneously under different storage conditions. Temperature, humidity, and light conditions were evaluated. Comparative analyses of methanol extracts were conducted using high performance liquid chromatographydiode array detection for chlorogenic acid, rutin, hyperoside, isoquercitrin, quercitrin, quercetin, amentoflavone, pseudohypericin, hyperforin, and hypericin. Analysis and extraction were performed using a validated method. The fluctuation of the constituents of the plant extract has been demonstrated. Among these components, chlorogenic acid was the most stable. Hyperforin, hypericin, and pseudohypericin were more stable than the flavonoids at −20°C, in the 6(th) month. As estimated, decay was lowest at −20°C and highest at 40°C–75% relative humidity for the analyzed constituents. Except for hyperforin, light protection decreased the breakdown of components within 4 months. However, at the 6(th) month, equivalent changes were seen for all constituents. Degradation of the constituents at −20°C indicates the importance of stability tests in analysis studies covering time and storage conditions. Taiwan Food and Drug Administration 2015-05-20 /pmc/articles/PMC9351782/ /pubmed/28911714 http://dx.doi.org/10.1016/j.jfda.2015.04.002 Text en © 2015 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Original Article Koyu, Halil Haznedaroglu, Mehmet Zeki Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract |
title | Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract |
title_full | Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract |
title_fullStr | Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract |
title_full_unstemmed | Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract |
title_short | Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract |
title_sort | investigation of impact of storage conditions on hypericum perforatum l. dried total extract |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351782/ https://www.ncbi.nlm.nih.gov/pubmed/28911714 http://dx.doi.org/10.1016/j.jfda.2015.04.002 |
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