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Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract

Hypericum perforatum L. (Hypericaceae) has been widely prescribed for mild to moderate depression following the release of promising results in clinical trials. However, it is known that its constituents may be affected by milieu. The stability complexities of the constituents of H. perforatum have...

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Autores principales: Koyu, Halil, Haznedaroglu, Mehmet Zeki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351782/
https://www.ncbi.nlm.nih.gov/pubmed/28911714
http://dx.doi.org/10.1016/j.jfda.2015.04.002
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author Koyu, Halil
Haznedaroglu, Mehmet Zeki
author_facet Koyu, Halil
Haznedaroglu, Mehmet Zeki
author_sort Koyu, Halil
collection PubMed
description Hypericum perforatum L. (Hypericaceae) has been widely prescribed for mild to moderate depression following the release of promising results in clinical trials. However, it is known that its constituents may be affected by milieu. The stability complexities of the constituents of H. perforatum have gained interest in recent years. The aim of the present study was to examine the impact of storage conditions on H. perforatum total extract simultaneously under different storage conditions. Temperature, humidity, and light conditions were evaluated. Comparative analyses of methanol extracts were conducted using high performance liquid chromatographydiode array detection for chlorogenic acid, rutin, hyperoside, isoquercitrin, quercitrin, quercetin, amentoflavone, pseudohypericin, hyperforin, and hypericin. Analysis and extraction were performed using a validated method. The fluctuation of the constituents of the plant extract has been demonstrated. Among these components, chlorogenic acid was the most stable. Hyperforin, hypericin, and pseudohypericin were more stable than the flavonoids at −20°C, in the 6(th) month. As estimated, decay was lowest at −20°C and highest at 40°C–75% relative humidity for the analyzed constituents. Except for hyperforin, light protection decreased the breakdown of components within 4 months. However, at the 6(th) month, equivalent changes were seen for all constituents. Degradation of the constituents at −20°C indicates the importance of stability tests in analysis studies covering time and storage conditions.
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spelling pubmed-93517822022-08-09 Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract Koyu, Halil Haznedaroglu, Mehmet Zeki J Food Drug Anal Original Article Hypericum perforatum L. (Hypericaceae) has been widely prescribed for mild to moderate depression following the release of promising results in clinical trials. However, it is known that its constituents may be affected by milieu. The stability complexities of the constituents of H. perforatum have gained interest in recent years. The aim of the present study was to examine the impact of storage conditions on H. perforatum total extract simultaneously under different storage conditions. Temperature, humidity, and light conditions were evaluated. Comparative analyses of methanol extracts were conducted using high performance liquid chromatographydiode array detection for chlorogenic acid, rutin, hyperoside, isoquercitrin, quercitrin, quercetin, amentoflavone, pseudohypericin, hyperforin, and hypericin. Analysis and extraction were performed using a validated method. The fluctuation of the constituents of the plant extract has been demonstrated. Among these components, chlorogenic acid was the most stable. Hyperforin, hypericin, and pseudohypericin were more stable than the flavonoids at −20°C, in the 6(th) month. As estimated, decay was lowest at −20°C and highest at 40°C–75% relative humidity for the analyzed constituents. Except for hyperforin, light protection decreased the breakdown of components within 4 months. However, at the 6(th) month, equivalent changes were seen for all constituents. Degradation of the constituents at −20°C indicates the importance of stability tests in analysis studies covering time and storage conditions. Taiwan Food and Drug Administration 2015-05-20 /pmc/articles/PMC9351782/ /pubmed/28911714 http://dx.doi.org/10.1016/j.jfda.2015.04.002 Text en © 2015 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Koyu, Halil
Haznedaroglu, Mehmet Zeki
Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract
title Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract
title_full Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract
title_fullStr Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract
title_full_unstemmed Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract
title_short Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract
title_sort investigation of impact of storage conditions on hypericum perforatum l. dried total extract
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351782/
https://www.ncbi.nlm.nih.gov/pubmed/28911714
http://dx.doi.org/10.1016/j.jfda.2015.04.002
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