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Development of an observational exposure human biomonitoring study to assess Canadian children’s DEET exposure during protective use

Biomonitoring data of N,N-diethyl-meta-toluamide (DEET) in children is scarce and limited to controlled exposure and surveillance studies. We conducted a 24-hour observational exposure and human biomonitoring study designed to estimate use of and exposure to DEET-based insect repellents by Canadian...

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Autores principales: Gibson, Jennifer C., Marro, Leonora, Borghese, Michael M., Brandow, Danielle, Remedios, Lauren, Fisher, Mandy, Malowany, Morie, Kieliszkiewicz, Katarzyna, Lukina, Anna O., Irwin, Kim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352095/
https://www.ncbi.nlm.nih.gov/pubmed/35925987
http://dx.doi.org/10.1371/journal.pone.0268341
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author Gibson, Jennifer C.
Marro, Leonora
Borghese, Michael M.
Brandow, Danielle
Remedios, Lauren
Fisher, Mandy
Malowany, Morie
Kieliszkiewicz, Katarzyna
Lukina, Anna O.
Irwin, Kim
author_facet Gibson, Jennifer C.
Marro, Leonora
Borghese, Michael M.
Brandow, Danielle
Remedios, Lauren
Fisher, Mandy
Malowany, Morie
Kieliszkiewicz, Katarzyna
Lukina, Anna O.
Irwin, Kim
author_sort Gibson, Jennifer C.
collection PubMed
description Biomonitoring data of N,N-diethyl-meta-toluamide (DEET) in children is scarce and limited to controlled exposure and surveillance studies. We conducted a 24-hour observational exposure and human biomonitoring study designed to estimate use of and exposure to DEET-based insect repellents by Canadian children in an overnight summer camp setting. Here, we present our study design and methodology. In 2019, children between the ages of 7 and 13 took part in the study (n = 126). Children controlled their use of DEET-based insect repellents, and provided an account of their activities at camp that could impact insect repellent absorption. Children provided a total of 389 urine samples throughout the study day, and reported the time that they applied insect repellent, which allowed us to contextualize urinary DEET and metabolite concentrations with respect to the timing of insect repellent application. DEET (2.3% <Limits of detection (LOD)) and two metabolites, N,N-diethyl-m-(hydroxymethyl)benzamide (DHMB) (0% <LOD) and 3-diethylcarbamoyl benzoic acid (DCBA) (0% <LOD), were measured in urine samples. Three time difference scenarios were established for the data and analysed to account for these complex time-dependent data, which demonstrated the need for DEET biomonitoring to be done in context with the timing of a known DEET exposure or over the course of at least 14 to 24 hours to better capture the excretion curve. To our knowledge, this is the first field-based study of real-world exposure to DEET in children. Our experience and results suggest that this type of real-world observational exposure study with a human biomonitoring component can generate data reflective of actual exposure, but is not without significant logistic, practical, and analytical challenges.
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spelling pubmed-93520952022-08-05 Development of an observational exposure human biomonitoring study to assess Canadian children’s DEET exposure during protective use Gibson, Jennifer C. Marro, Leonora Borghese, Michael M. Brandow, Danielle Remedios, Lauren Fisher, Mandy Malowany, Morie Kieliszkiewicz, Katarzyna Lukina, Anna O. Irwin, Kim PLoS One Research Article Biomonitoring data of N,N-diethyl-meta-toluamide (DEET) in children is scarce and limited to controlled exposure and surveillance studies. We conducted a 24-hour observational exposure and human biomonitoring study designed to estimate use of and exposure to DEET-based insect repellents by Canadian children in an overnight summer camp setting. Here, we present our study design and methodology. In 2019, children between the ages of 7 and 13 took part in the study (n = 126). Children controlled their use of DEET-based insect repellents, and provided an account of their activities at camp that could impact insect repellent absorption. Children provided a total of 389 urine samples throughout the study day, and reported the time that they applied insect repellent, which allowed us to contextualize urinary DEET and metabolite concentrations with respect to the timing of insect repellent application. DEET (2.3% <Limits of detection (LOD)) and two metabolites, N,N-diethyl-m-(hydroxymethyl)benzamide (DHMB) (0% <LOD) and 3-diethylcarbamoyl benzoic acid (DCBA) (0% <LOD), were measured in urine samples. Three time difference scenarios were established for the data and analysed to account for these complex time-dependent data, which demonstrated the need for DEET biomonitoring to be done in context with the timing of a known DEET exposure or over the course of at least 14 to 24 hours to better capture the excretion curve. To our knowledge, this is the first field-based study of real-world exposure to DEET in children. Our experience and results suggest that this type of real-world observational exposure study with a human biomonitoring component can generate data reflective of actual exposure, but is not without significant logistic, practical, and analytical challenges. Public Library of Science 2022-08-04 /pmc/articles/PMC9352095/ /pubmed/35925987 http://dx.doi.org/10.1371/journal.pone.0268341 Text en © 2022 Health Canada and Gibson et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gibson, Jennifer C.
Marro, Leonora
Borghese, Michael M.
Brandow, Danielle
Remedios, Lauren
Fisher, Mandy
Malowany, Morie
Kieliszkiewicz, Katarzyna
Lukina, Anna O.
Irwin, Kim
Development of an observational exposure human biomonitoring study to assess Canadian children’s DEET exposure during protective use
title Development of an observational exposure human biomonitoring study to assess Canadian children’s DEET exposure during protective use
title_full Development of an observational exposure human biomonitoring study to assess Canadian children’s DEET exposure during protective use
title_fullStr Development of an observational exposure human biomonitoring study to assess Canadian children’s DEET exposure during protective use
title_full_unstemmed Development of an observational exposure human biomonitoring study to assess Canadian children’s DEET exposure during protective use
title_short Development of an observational exposure human biomonitoring study to assess Canadian children’s DEET exposure during protective use
title_sort development of an observational exposure human biomonitoring study to assess canadian children’s deet exposure during protective use
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352095/
https://www.ncbi.nlm.nih.gov/pubmed/35925987
http://dx.doi.org/10.1371/journal.pone.0268341
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