Cargando…

Multi-Domains in a Single Lattice Formed by DNA Self-Assembly

[Image: see text] Using sequence programmability and the characteristics of self-assembly, DNA has been utilized in the construction of various nanostructures and the placement of specific patterns on lattices. Even though many complex structures and patterns formed by DNA assembly have been reporte...

Descripción completa

Detalles Bibliográficos
Autores principales: Jo, Soojin, Lee, Sungjin, Park, Suyoun, Tandon, Anshula, Nguyen, Thi Bich Ngoc, Vu, Thi Hong Nhung, Raza, Muhammad Tayyab, Park, Sung Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352336/
https://www.ncbi.nlm.nih.gov/pubmed/35936484
http://dx.doi.org/10.1021/acsomega.2c02556
_version_ 1784762631645560832
author Jo, Soojin
Lee, Sungjin
Park, Suyoun
Tandon, Anshula
Nguyen, Thi Bich Ngoc
Vu, Thi Hong Nhung
Raza, Muhammad Tayyab
Park, Sung Ha
author_facet Jo, Soojin
Lee, Sungjin
Park, Suyoun
Tandon, Anshula
Nguyen, Thi Bich Ngoc
Vu, Thi Hong Nhung
Raza, Muhammad Tayyab
Park, Sung Ha
author_sort Jo, Soojin
collection PubMed
description [Image: see text] Using sequence programmability and the characteristics of self-assembly, DNA has been utilized in the construction of various nanostructures and the placement of specific patterns on lattices. Even though many complex structures and patterns formed by DNA assembly have been reported, the fabrication of multi-domain patterns in a single lattice has rarely been discussed. Multi-domains possessing specifically designed patterns in a single lattice provide the possibility to generate multiple patterns that enhance the pattern density in a given single lattice. Here, we introduce boundaries to construct double- and quadruple-domains with specific patterns in a single lattice and verify them with atomic force microscopy. ON, OFF, and ST (stripe) patterns on a lattice are made of DNA tiles with hairpins (ON), without hairpins (OFF), and alternating DNA tiles without and with hairpins (formed as a stripe, ST). For double- and quadruple-domain lattices, linear and cross boundaries were designed to fabricate two (e.g., ON and OFF, ON and ST, and OFF and ST) and four (OFF, ST, OFF, and ON) different types of patterns in single lattices, respectively. In double-domain lattices, each linear boundary is placed between two different domains. Similarly, four linear boundaries connected with a seed tile (i.e., a cross boundary) can separate four domains in a single lattice in quadruple-domain lattices. Due to the presence of boundaries, the pattern growth directions are different in each domain. The experimentally obtained multi-domain patterns agree well with our design. Lastly, we propose the possibility of the construction of a hexadomain lattice through the mapping from hexagonal to square grids converted by using an axial coordinate system. By proposing a hexadomain lattice design, we anticipate the possibility to extend to higher numbers of multi-domains in a single lattice, thereby further increasing the information density in a given lattice.
format Online
Article
Text
id pubmed-9352336
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-93523362022-08-05 Multi-Domains in a Single Lattice Formed by DNA Self-Assembly Jo, Soojin Lee, Sungjin Park, Suyoun Tandon, Anshula Nguyen, Thi Bich Ngoc Vu, Thi Hong Nhung Raza, Muhammad Tayyab Park, Sung Ha ACS Omega [Image: see text] Using sequence programmability and the characteristics of self-assembly, DNA has been utilized in the construction of various nanostructures and the placement of specific patterns on lattices. Even though many complex structures and patterns formed by DNA assembly have been reported, the fabrication of multi-domain patterns in a single lattice has rarely been discussed. Multi-domains possessing specifically designed patterns in a single lattice provide the possibility to generate multiple patterns that enhance the pattern density in a given single lattice. Here, we introduce boundaries to construct double- and quadruple-domains with specific patterns in a single lattice and verify them with atomic force microscopy. ON, OFF, and ST (stripe) patterns on a lattice are made of DNA tiles with hairpins (ON), without hairpins (OFF), and alternating DNA tiles without and with hairpins (formed as a stripe, ST). For double- and quadruple-domain lattices, linear and cross boundaries were designed to fabricate two (e.g., ON and OFF, ON and ST, and OFF and ST) and four (OFF, ST, OFF, and ON) different types of patterns in single lattices, respectively. In double-domain lattices, each linear boundary is placed between two different domains. Similarly, four linear boundaries connected with a seed tile (i.e., a cross boundary) can separate four domains in a single lattice in quadruple-domain lattices. Due to the presence of boundaries, the pattern growth directions are different in each domain. The experimentally obtained multi-domain patterns agree well with our design. Lastly, we propose the possibility of the construction of a hexadomain lattice through the mapping from hexagonal to square grids converted by using an axial coordinate system. By proposing a hexadomain lattice design, we anticipate the possibility to extend to higher numbers of multi-domains in a single lattice, thereby further increasing the information density in a given lattice. American Chemical Society 2022-07-19 /pmc/articles/PMC9352336/ /pubmed/35936484 http://dx.doi.org/10.1021/acsomega.2c02556 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Jo, Soojin
Lee, Sungjin
Park, Suyoun
Tandon, Anshula
Nguyen, Thi Bich Ngoc
Vu, Thi Hong Nhung
Raza, Muhammad Tayyab
Park, Sung Ha
Multi-Domains in a Single Lattice Formed by DNA Self-Assembly
title Multi-Domains in a Single Lattice Formed by DNA Self-Assembly
title_full Multi-Domains in a Single Lattice Formed by DNA Self-Assembly
title_fullStr Multi-Domains in a Single Lattice Formed by DNA Self-Assembly
title_full_unstemmed Multi-Domains in a Single Lattice Formed by DNA Self-Assembly
title_short Multi-Domains in a Single Lattice Formed by DNA Self-Assembly
title_sort multi-domains in a single lattice formed by dna self-assembly
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352336/
https://www.ncbi.nlm.nih.gov/pubmed/35936484
http://dx.doi.org/10.1021/acsomega.2c02556
work_keys_str_mv AT josoojin multidomainsinasinglelatticeformedbydnaselfassembly
AT leesungjin multidomainsinasinglelatticeformedbydnaselfassembly
AT parksuyoun multidomainsinasinglelatticeformedbydnaselfassembly
AT tandonanshula multidomainsinasinglelatticeformedbydnaselfassembly
AT nguyenthibichngoc multidomainsinasinglelatticeformedbydnaselfassembly
AT vuthihongnhung multidomainsinasinglelatticeformedbydnaselfassembly
AT razamuhammadtayyab multidomainsinasinglelatticeformedbydnaselfassembly
AT parksungha multidomainsinasinglelatticeformedbydnaselfassembly