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A comparative study of microRNAs in different stages of Eimeria tenella

Apicomplexan parasites have divergent biogenesis machinery for small RNA generation. Analysis has shown that parasites in Plasmodium and Cryptosporidium as well as many species in Leishmania or Trypanosoma do not have a complete machinery in small RNA biogenesis. Recently, the miRNA-generating syste...

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Autores principales: Zhang, Lei, Chen, Linlin, Zhang, Hongtao, Si, Hongbin, Liu, Xianyong, Suo, Xun, Hu, Dandan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353057/
https://www.ncbi.nlm.nih.gov/pubmed/35937295
http://dx.doi.org/10.3389/fvets.2022.954725
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author Zhang, Lei
Chen, Linlin
Zhang, Hongtao
Si, Hongbin
Liu, Xianyong
Suo, Xun
Hu, Dandan
author_facet Zhang, Lei
Chen, Linlin
Zhang, Hongtao
Si, Hongbin
Liu, Xianyong
Suo, Xun
Hu, Dandan
author_sort Zhang, Lei
collection PubMed
description Apicomplexan parasites have divergent biogenesis machinery for small RNA generation. Analysis has shown that parasites in Plasmodium and Cryptosporidium as well as many species in Leishmania or Trypanosoma do not have a complete machinery in small RNA biogenesis. Recently, the miRNA-generating system of Toxoplasma has been identified as plant/fungal-like and its miRNAome has been elucidated. However, the microRNA (miRNA) expression profiles and their potential regulatory functions in different stages of Eimeria tenella remain largely unknown. In this study, we characterized the RNA silencing machinery of E. tenella and investigated the miRNA population distribution at different life stages by high-throughput sequencing. We characterized the expression of miRNAs in the unsporulated oocyst, sporulated oocyst and schizogony stages, obtaining a total of 392 miRNAs. We identified 58 differentially expressed miRNAs between USO (unsporulated oocysts) and SO (sporulated oocysts) that were significantly enriched for their potential target genes in the regulation of gene expression and chromatin binding, suggesting an epigenetic modulation of sporulating by these miRNAs. In comparing miRNA expression at endogenous and exogenous developmental stages, twenty-four miRNAs were identified differently expressed. Those were mainly associated with the regulation of genes with protein kinase activity, suggesting control of protein phosphorylation. This is the first study about the evolution of miRNA biogenesis system and miRNA control of gene expression in Eimeria species. Our data may lead to functional insights into of the regulation of gene expression during parasite life cycle in apicomplexan parasites.
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spelling pubmed-93530572022-08-06 A comparative study of microRNAs in different stages of Eimeria tenella Zhang, Lei Chen, Linlin Zhang, Hongtao Si, Hongbin Liu, Xianyong Suo, Xun Hu, Dandan Front Vet Sci Veterinary Science Apicomplexan parasites have divergent biogenesis machinery for small RNA generation. Analysis has shown that parasites in Plasmodium and Cryptosporidium as well as many species in Leishmania or Trypanosoma do not have a complete machinery in small RNA biogenesis. Recently, the miRNA-generating system of Toxoplasma has been identified as plant/fungal-like and its miRNAome has been elucidated. However, the microRNA (miRNA) expression profiles and their potential regulatory functions in different stages of Eimeria tenella remain largely unknown. In this study, we characterized the RNA silencing machinery of E. tenella and investigated the miRNA population distribution at different life stages by high-throughput sequencing. We characterized the expression of miRNAs in the unsporulated oocyst, sporulated oocyst and schizogony stages, obtaining a total of 392 miRNAs. We identified 58 differentially expressed miRNAs between USO (unsporulated oocysts) and SO (sporulated oocysts) that were significantly enriched for their potential target genes in the regulation of gene expression and chromatin binding, suggesting an epigenetic modulation of sporulating by these miRNAs. In comparing miRNA expression at endogenous and exogenous developmental stages, twenty-four miRNAs were identified differently expressed. Those were mainly associated with the regulation of genes with protein kinase activity, suggesting control of protein phosphorylation. This is the first study about the evolution of miRNA biogenesis system and miRNA control of gene expression in Eimeria species. Our data may lead to functional insights into of the regulation of gene expression during parasite life cycle in apicomplexan parasites. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9353057/ /pubmed/35937295 http://dx.doi.org/10.3389/fvets.2022.954725 Text en Copyright © 2022 Zhang, Chen, Zhang, Si, Liu, Suo and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zhang, Lei
Chen, Linlin
Zhang, Hongtao
Si, Hongbin
Liu, Xianyong
Suo, Xun
Hu, Dandan
A comparative study of microRNAs in different stages of Eimeria tenella
title A comparative study of microRNAs in different stages of Eimeria tenella
title_full A comparative study of microRNAs in different stages of Eimeria tenella
title_fullStr A comparative study of microRNAs in different stages of Eimeria tenella
title_full_unstemmed A comparative study of microRNAs in different stages of Eimeria tenella
title_short A comparative study of microRNAs in different stages of Eimeria tenella
title_sort comparative study of micrornas in different stages of eimeria tenella
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353057/
https://www.ncbi.nlm.nih.gov/pubmed/35937295
http://dx.doi.org/10.3389/fvets.2022.954725
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