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SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression
Drought stress limits plant development and reproduction. Multiple mechanisms in plants are activated to respond to stress. The MYC2 transcription factor is a core regulator of the jasmonate (JA) pathway and plays a vital role in the crosstalk between abscisic acid (ABA) and JA. In this study, we fo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354244/ https://www.ncbi.nlm.nih.gov/pubmed/35937339 http://dx.doi.org/10.3389/fpls.2022.952758 |
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author | Xu, Bing-Qin Wang, Jing-Jing Peng, Yi Huang, Huang Sun, Lu-Lu Yang, Rui Suo, Lin-Na Wang, Shao-Hui Zhao, Wen-Chao |
author_facet | Xu, Bing-Qin Wang, Jing-Jing Peng, Yi Huang, Huang Sun, Lu-Lu Yang, Rui Suo, Lin-Na Wang, Shao-Hui Zhao, Wen-Chao |
author_sort | Xu, Bing-Qin |
collection | PubMed |
description | Drought stress limits plant development and reproduction. Multiple mechanisms in plants are activated to respond to stress. The MYC2 transcription factor is a core regulator of the jasmonate (JA) pathway and plays a vital role in the crosstalk between abscisic acid (ABA) and JA. In this study, we found that SlMYC2 responded to drought stress and regulated stomatal aperture in tomato (Solanum lycopersicum). Overexpression of SlMYC2 repressed SlCHS1 expression and decreased the flavonol content, increased the reactive oxygen species (ROS) content in guard cells and promoted the accumulation of JA and ABA in leaves. Additionally, silencing the SlCHS1 gene produced a phenotype that was similar to that of the MYC2-overexpressing (MYC2-OE) strain, especially in terms of stomatal dynamics and ROS levels. Finally, we confirmed that SlMYC2 directly repressed the expression of SlCHS1. Our study revealed that SlMYC2 drove stomatal closure by modulating the accumulation of flavonol and the JA and ABA contents, helping us decipher the mechanism of stomatal movement under drought stress. |
format | Online Article Text |
id | pubmed-9354244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93542442022-08-06 SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression Xu, Bing-Qin Wang, Jing-Jing Peng, Yi Huang, Huang Sun, Lu-Lu Yang, Rui Suo, Lin-Na Wang, Shao-Hui Zhao, Wen-Chao Front Plant Sci Plant Science Drought stress limits plant development and reproduction. Multiple mechanisms in plants are activated to respond to stress. The MYC2 transcription factor is a core regulator of the jasmonate (JA) pathway and plays a vital role in the crosstalk between abscisic acid (ABA) and JA. In this study, we found that SlMYC2 responded to drought stress and regulated stomatal aperture in tomato (Solanum lycopersicum). Overexpression of SlMYC2 repressed SlCHS1 expression and decreased the flavonol content, increased the reactive oxygen species (ROS) content in guard cells and promoted the accumulation of JA and ABA in leaves. Additionally, silencing the SlCHS1 gene produced a phenotype that was similar to that of the MYC2-overexpressing (MYC2-OE) strain, especially in terms of stomatal dynamics and ROS levels. Finally, we confirmed that SlMYC2 directly repressed the expression of SlCHS1. Our study revealed that SlMYC2 drove stomatal closure by modulating the accumulation of flavonol and the JA and ABA contents, helping us decipher the mechanism of stomatal movement under drought stress. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9354244/ /pubmed/35937339 http://dx.doi.org/10.3389/fpls.2022.952758 Text en Copyright © 2022 Xu, Wang, Peng, Huang, Sun, Yang, Suo, Wang and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Xu, Bing-Qin Wang, Jing-Jing Peng, Yi Huang, Huang Sun, Lu-Lu Yang, Rui Suo, Lin-Na Wang, Shao-Hui Zhao, Wen-Chao SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression |
title | SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression |
title_full | SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression |
title_fullStr | SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression |
title_full_unstemmed | SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression |
title_short | SlMYC2 mediates stomatal movement in response to drought stress by repressing SlCHS1 expression |
title_sort | slmyc2 mediates stomatal movement in response to drought stress by repressing slchs1 expression |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354244/ https://www.ncbi.nlm.nih.gov/pubmed/35937339 http://dx.doi.org/10.3389/fpls.2022.952758 |
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