Cargando…
Precision Navigation of Hepatic Ischemia–Reperfusion Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence
[Image: see text] Hepatic ischemia–reperfusion injury (HIRI) is responsible for postoperative liver dysfunction and liver failure. Precise and rapid navigation of HIRI lesions is critical for early warning and timely development of pretreatment plans. Available methods for assaying liver injury fail...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354259/ https://www.ncbi.nlm.nih.gov/pubmed/35793548 http://dx.doi.org/10.1021/jacs.2c03832 |
_version_ | 1784763027456786432 |
---|---|
author | Liu, Jihong Zhang, Wen Zhou, Chunmiao Li, Mengmei Wang, Xin Zhang, Wei Liu, Zhenzhen Wu, Luling James, Tony D. Li, Ping Tang, Bo |
author_facet | Liu, Jihong Zhang, Wen Zhou, Chunmiao Li, Mengmei Wang, Xin Zhang, Wei Liu, Zhenzhen Wu, Luling James, Tony D. Li, Ping Tang, Bo |
author_sort | Liu, Jihong |
collection | PubMed |
description | [Image: see text] Hepatic ischemia–reperfusion injury (HIRI) is responsible for postoperative liver dysfunction and liver failure. Precise and rapid navigation of HIRI lesions is critical for early warning and timely development of pretreatment plans. Available methods for assaying liver injury fail to provide the exact location of lesions in real time intraoperatively. HIRI is intimately associated with oxidative stress which impairs lysosomal degradative function, leading to significant changes in lysosomal viscosity. Therefore, lysosomal viscosity is a potential biomarker for the precise targeting of HIRI. Hence, we developed a viscosity-activatable second near-infrared window fluorescent probe (NP-V) for the detection of lysosomal viscosity in hepatocytes and mice during HIRI. A reactive oxygen species–malondialdehyde–cathepsin B signaling pathway during HIRI was established. We further conducted high signal-to-background ratio NIR-II fluorescence imaging of HIRI mice. The contour and boundary of liver lesions were delineated, and as such the precise intraoperative resection of the lesion area was implemented. This research demonstrates the potential of NP-V as a dual-functional probe for the elucidation of HIRI pathogenesis and the direct navigation of HIRI lesions in clinical applications. |
format | Online Article Text |
id | pubmed-9354259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93542592022-08-06 Precision Navigation of Hepatic Ischemia–Reperfusion Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence Liu, Jihong Zhang, Wen Zhou, Chunmiao Li, Mengmei Wang, Xin Zhang, Wei Liu, Zhenzhen Wu, Luling James, Tony D. Li, Ping Tang, Bo J Am Chem Soc [Image: see text] Hepatic ischemia–reperfusion injury (HIRI) is responsible for postoperative liver dysfunction and liver failure. Precise and rapid navigation of HIRI lesions is critical for early warning and timely development of pretreatment plans. Available methods for assaying liver injury fail to provide the exact location of lesions in real time intraoperatively. HIRI is intimately associated with oxidative stress which impairs lysosomal degradative function, leading to significant changes in lysosomal viscosity. Therefore, lysosomal viscosity is a potential biomarker for the precise targeting of HIRI. Hence, we developed a viscosity-activatable second near-infrared window fluorescent probe (NP-V) for the detection of lysosomal viscosity in hepatocytes and mice during HIRI. A reactive oxygen species–malondialdehyde–cathepsin B signaling pathway during HIRI was established. We further conducted high signal-to-background ratio NIR-II fluorescence imaging of HIRI mice. The contour and boundary of liver lesions were delineated, and as such the precise intraoperative resection of the lesion area was implemented. This research demonstrates the potential of NP-V as a dual-functional probe for the elucidation of HIRI pathogenesis and the direct navigation of HIRI lesions in clinical applications. American Chemical Society 2022-07-06 2022-08-03 /pmc/articles/PMC9354259/ /pubmed/35793548 http://dx.doi.org/10.1021/jacs.2c03832 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Liu, Jihong Zhang, Wen Zhou, Chunmiao Li, Mengmei Wang, Xin Zhang, Wei Liu, Zhenzhen Wu, Luling James, Tony D. Li, Ping Tang, Bo Precision Navigation of Hepatic Ischemia–Reperfusion Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence |
title | Precision
Navigation of Hepatic Ischemia–Reperfusion
Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence |
title_full | Precision
Navigation of Hepatic Ischemia–Reperfusion
Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence |
title_fullStr | Precision
Navigation of Hepatic Ischemia–Reperfusion
Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence |
title_full_unstemmed | Precision
Navigation of Hepatic Ischemia–Reperfusion
Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence |
title_short | Precision
Navigation of Hepatic Ischemia–Reperfusion
Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence |
title_sort | precision
navigation of hepatic ischemia–reperfusion
injury guided by lysosomal viscosity-activatable nir-ii fluorescence |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354259/ https://www.ncbi.nlm.nih.gov/pubmed/35793548 http://dx.doi.org/10.1021/jacs.2c03832 |
work_keys_str_mv | AT liujihong precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT zhangwen precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT zhouchunmiao precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT limengmei precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT wangxin precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT zhangwei precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT liuzhenzhen precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT wululing precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT jamestonyd precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT liping precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence AT tangbo precisionnavigationofhepaticischemiareperfusioninjuryguidedbylysosomalviscosityactivatableniriifluorescence |