Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research

Three induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal human dermal fibroblasts (HDFs) derived from a human skin punch biopsy. The biopsy was donated from a woman with known infertility due to ovarian failure. The hiPSC sublines were created using Sendai virus vectors...

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Autores principales: Pandolfi, Erica C., Hunt, Timothy J., Goldsmith, Sierra, Hurlbut, Kellie, Silber, Sherman J., Clark, Amander T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354709/
https://www.ncbi.nlm.nih.gov/pubmed/34216981
http://dx.doi.org/10.1016/j.scr.2021.102446
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author Pandolfi, Erica C.
Hunt, Timothy J.
Goldsmith, Sierra
Hurlbut, Kellie
Silber, Sherman J.
Clark, Amander T.
author_facet Pandolfi, Erica C.
Hunt, Timothy J.
Goldsmith, Sierra
Hurlbut, Kellie
Silber, Sherman J.
Clark, Amander T.
author_sort Pandolfi, Erica C.
collection PubMed
description Three induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal human dermal fibroblasts (HDFs) derived from a human skin punch biopsy. The biopsy was donated from a woman with known infertility due to ovarian failure. The hiPSC sublines were created using Sendai virus vectors and were positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was verified using PluriTest analysis and in vitro differentiation using Taqman Real-Time PCR assays for somatic lineage markers. This participant’s monozygotic twin sister also donated a skin-punch biopsy, whose resulting hiPSC lines were published previously as a resource.
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spelling pubmed-93547092022-08-05 Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research Pandolfi, Erica C. Hunt, Timothy J. Goldsmith, Sierra Hurlbut, Kellie Silber, Sherman J. Clark, Amander T. Stem Cell Res Article Three induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal human dermal fibroblasts (HDFs) derived from a human skin punch biopsy. The biopsy was donated from a woman with known infertility due to ovarian failure. The hiPSC sublines were created using Sendai virus vectors and were positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was verified using PluriTest analysis and in vitro differentiation using Taqman Real-Time PCR assays for somatic lineage markers. This participant’s monozygotic twin sister also donated a skin-punch biopsy, whose resulting hiPSC lines were published previously as a resource. 2021-07 2021-06-24 /pmc/articles/PMC9354709/ /pubmed/34216981 http://dx.doi.org/10.1016/j.scr.2021.102446 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Pandolfi, Erica C.
Hunt, Timothy J.
Goldsmith, Sierra
Hurlbut, Kellie
Silber, Sherman J.
Clark, Amander T.
Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
title Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
title_full Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
title_fullStr Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
title_full_unstemmed Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
title_short Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research
title_sort generation of three human induced pluripotent stem cell sublines (uclai004-a, uclai004-b, and uclai004-c) for reproductive science research
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354709/
https://www.ncbi.nlm.nih.gov/pubmed/34216981
http://dx.doi.org/10.1016/j.scr.2021.102446
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