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Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene

Rice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most destructive diseases threatening rice production worldwide. Development of resistant cultivars using broad-spectrum resistance (R) genes with high breeding value is the most effective and economical approach to control this dis...

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Autores principales: Feng, Zhiming, Li, Mingyou, Xu, Zhiwen, Gao, Peng, Wu, Yunyu, Wu, Keting, Zhao, Jianhua, Wang, Xiaoqiu, Wang, Jianan, Li, Mengchen, Hu, Keming, Chen, Hongqi, Deng, Yiwen, Li, Aihong, Chen, Zongxiang, Zuo, Shimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354813/
https://www.ncbi.nlm.nih.gov/pubmed/35937342
http://dx.doi.org/10.3389/fpls.2022.937767
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author Feng, Zhiming
Li, Mingyou
Xu, Zhiwen
Gao, Peng
Wu, Yunyu
Wu, Keting
Zhao, Jianhua
Wang, Xiaoqiu
Wang, Jianan
Li, Mengchen
Hu, Keming
Chen, Hongqi
Deng, Yiwen
Li, Aihong
Chen, Zongxiang
Zuo, Shimin
author_facet Feng, Zhiming
Li, Mingyou
Xu, Zhiwen
Gao, Peng
Wu, Yunyu
Wu, Keting
Zhao, Jianhua
Wang, Xiaoqiu
Wang, Jianan
Li, Mengchen
Hu, Keming
Chen, Hongqi
Deng, Yiwen
Li, Aihong
Chen, Zongxiang
Zuo, Shimin
author_sort Feng, Zhiming
collection PubMed
description Rice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most destructive diseases threatening rice production worldwide. Development of resistant cultivars using broad-spectrum resistance (R) genes with high breeding value is the most effective and economical approach to control this disease. In this study, the breeding potential of Pigm gene in geng/japonica rice breeding practice in Jiangsu province was comprehensively evaluated. Through backcross and marker-assisted selection (MAS), Pigm was introduced into two geng rice cultivars (Wuyungeng 32/WYG32 and Huageng 8/HG8). In each genetic background, five advanced backcross lines with Pigm (ABLs) and the same genotypes as the respective recurrent parent in the other 13 known R gene loci were developed. Compared with the corresponding recurrent parent, all these ABLs exhibited stronger resistance in seedling inoculation assay using 184 isolates collected from rice growing regions of the lower region of the Yangtze River. With respect to panicle blast resistance, all ABLs reached a high resistance level to blast disease in tests conducted in three consecutive years with the inoculation of seven mixed conidial suspensions collected from different regions of Jiangsu province. In natural field nursery assays, the ABLs showed significantly higher resistance than the recurrent parents. No common change on importantly morphological traits and yield-associated components was found among the ABLs, demonstrating the introduction of Pigm had no tightly linked undesirable effect on rice economically important traits and its associated grain weight reduction effect could be probably offset by others grain weight genes or at least in the background of the aforementioned two varieties. Notably, one rice line with Pigm, designated as Yangnonggeng 3091, had been authorized as a new variety in Jiangsu province in 2021, showing excellent performance on both grain yield and quality, as well as the blast resistance. Together, these results suggest that the Pigm gene has a high breeding value in developing rice varieties with durable and broad-spectrum resistance to blast disease.
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spelling pubmed-93548132022-08-06 Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene Feng, Zhiming Li, Mingyou Xu, Zhiwen Gao, Peng Wu, Yunyu Wu, Keting Zhao, Jianhua Wang, Xiaoqiu Wang, Jianan Li, Mengchen Hu, Keming Chen, Hongqi Deng, Yiwen Li, Aihong Chen, Zongxiang Zuo, Shimin Front Plant Sci Plant Science Rice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most destructive diseases threatening rice production worldwide. Development of resistant cultivars using broad-spectrum resistance (R) genes with high breeding value is the most effective and economical approach to control this disease. In this study, the breeding potential of Pigm gene in geng/japonica rice breeding practice in Jiangsu province was comprehensively evaluated. Through backcross and marker-assisted selection (MAS), Pigm was introduced into two geng rice cultivars (Wuyungeng 32/WYG32 and Huageng 8/HG8). In each genetic background, five advanced backcross lines with Pigm (ABLs) and the same genotypes as the respective recurrent parent in the other 13 known R gene loci were developed. Compared with the corresponding recurrent parent, all these ABLs exhibited stronger resistance in seedling inoculation assay using 184 isolates collected from rice growing regions of the lower region of the Yangtze River. With respect to panicle blast resistance, all ABLs reached a high resistance level to blast disease in tests conducted in three consecutive years with the inoculation of seven mixed conidial suspensions collected from different regions of Jiangsu province. In natural field nursery assays, the ABLs showed significantly higher resistance than the recurrent parents. No common change on importantly morphological traits and yield-associated components was found among the ABLs, demonstrating the introduction of Pigm had no tightly linked undesirable effect on rice economically important traits and its associated grain weight reduction effect could be probably offset by others grain weight genes or at least in the background of the aforementioned two varieties. Notably, one rice line with Pigm, designated as Yangnonggeng 3091, had been authorized as a new variety in Jiangsu province in 2021, showing excellent performance on both grain yield and quality, as well as the blast resistance. Together, these results suggest that the Pigm gene has a high breeding value in developing rice varieties with durable and broad-spectrum resistance to blast disease. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9354813/ /pubmed/35937342 http://dx.doi.org/10.3389/fpls.2022.937767 Text en Copyright © 2022 Feng, Li, Xu, Gao, Wu, Wu, Zhao, Wang, Wang, Li, Hu, Chen, Deng, Li, Chen and Zuo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Feng, Zhiming
Li, Mingyou
Xu, Zhiwen
Gao, Peng
Wu, Yunyu
Wu, Keting
Zhao, Jianhua
Wang, Xiaoqiu
Wang, Jianan
Li, Mengchen
Hu, Keming
Chen, Hongqi
Deng, Yiwen
Li, Aihong
Chen, Zongxiang
Zuo, Shimin
Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene
title Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene
title_full Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene
title_fullStr Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene
title_full_unstemmed Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene
title_short Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene
title_sort development of rice variety with durable and broad-spectrum resistance to blast disease through marker-assisted introduction of pigm gene
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354813/
https://www.ncbi.nlm.nih.gov/pubmed/35937342
http://dx.doi.org/10.3389/fpls.2022.937767
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