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Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene
Rice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most destructive diseases threatening rice production worldwide. Development of resistant cultivars using broad-spectrum resistance (R) genes with high breeding value is the most effective and economical approach to control this dis...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354813/ https://www.ncbi.nlm.nih.gov/pubmed/35937342 http://dx.doi.org/10.3389/fpls.2022.937767 |
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author | Feng, Zhiming Li, Mingyou Xu, Zhiwen Gao, Peng Wu, Yunyu Wu, Keting Zhao, Jianhua Wang, Xiaoqiu Wang, Jianan Li, Mengchen Hu, Keming Chen, Hongqi Deng, Yiwen Li, Aihong Chen, Zongxiang Zuo, Shimin |
author_facet | Feng, Zhiming Li, Mingyou Xu, Zhiwen Gao, Peng Wu, Yunyu Wu, Keting Zhao, Jianhua Wang, Xiaoqiu Wang, Jianan Li, Mengchen Hu, Keming Chen, Hongqi Deng, Yiwen Li, Aihong Chen, Zongxiang Zuo, Shimin |
author_sort | Feng, Zhiming |
collection | PubMed |
description | Rice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most destructive diseases threatening rice production worldwide. Development of resistant cultivars using broad-spectrum resistance (R) genes with high breeding value is the most effective and economical approach to control this disease. In this study, the breeding potential of Pigm gene in geng/japonica rice breeding practice in Jiangsu province was comprehensively evaluated. Through backcross and marker-assisted selection (MAS), Pigm was introduced into two geng rice cultivars (Wuyungeng 32/WYG32 and Huageng 8/HG8). In each genetic background, five advanced backcross lines with Pigm (ABLs) and the same genotypes as the respective recurrent parent in the other 13 known R gene loci were developed. Compared with the corresponding recurrent parent, all these ABLs exhibited stronger resistance in seedling inoculation assay using 184 isolates collected from rice growing regions of the lower region of the Yangtze River. With respect to panicle blast resistance, all ABLs reached a high resistance level to blast disease in tests conducted in three consecutive years with the inoculation of seven mixed conidial suspensions collected from different regions of Jiangsu province. In natural field nursery assays, the ABLs showed significantly higher resistance than the recurrent parents. No common change on importantly morphological traits and yield-associated components was found among the ABLs, demonstrating the introduction of Pigm had no tightly linked undesirable effect on rice economically important traits and its associated grain weight reduction effect could be probably offset by others grain weight genes or at least in the background of the aforementioned two varieties. Notably, one rice line with Pigm, designated as Yangnonggeng 3091, had been authorized as a new variety in Jiangsu province in 2021, showing excellent performance on both grain yield and quality, as well as the blast resistance. Together, these results suggest that the Pigm gene has a high breeding value in developing rice varieties with durable and broad-spectrum resistance to blast disease. |
format | Online Article Text |
id | pubmed-9354813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93548132022-08-06 Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene Feng, Zhiming Li, Mingyou Xu, Zhiwen Gao, Peng Wu, Yunyu Wu, Keting Zhao, Jianhua Wang, Xiaoqiu Wang, Jianan Li, Mengchen Hu, Keming Chen, Hongqi Deng, Yiwen Li, Aihong Chen, Zongxiang Zuo, Shimin Front Plant Sci Plant Science Rice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most destructive diseases threatening rice production worldwide. Development of resistant cultivars using broad-spectrum resistance (R) genes with high breeding value is the most effective and economical approach to control this disease. In this study, the breeding potential of Pigm gene in geng/japonica rice breeding practice in Jiangsu province was comprehensively evaluated. Through backcross and marker-assisted selection (MAS), Pigm was introduced into two geng rice cultivars (Wuyungeng 32/WYG32 and Huageng 8/HG8). In each genetic background, five advanced backcross lines with Pigm (ABLs) and the same genotypes as the respective recurrent parent in the other 13 known R gene loci were developed. Compared with the corresponding recurrent parent, all these ABLs exhibited stronger resistance in seedling inoculation assay using 184 isolates collected from rice growing regions of the lower region of the Yangtze River. With respect to panicle blast resistance, all ABLs reached a high resistance level to blast disease in tests conducted in three consecutive years with the inoculation of seven mixed conidial suspensions collected from different regions of Jiangsu province. In natural field nursery assays, the ABLs showed significantly higher resistance than the recurrent parents. No common change on importantly morphological traits and yield-associated components was found among the ABLs, demonstrating the introduction of Pigm had no tightly linked undesirable effect on rice economically important traits and its associated grain weight reduction effect could be probably offset by others grain weight genes or at least in the background of the aforementioned two varieties. Notably, one rice line with Pigm, designated as Yangnonggeng 3091, had been authorized as a new variety in Jiangsu province in 2021, showing excellent performance on both grain yield and quality, as well as the blast resistance. Together, these results suggest that the Pigm gene has a high breeding value in developing rice varieties with durable and broad-spectrum resistance to blast disease. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9354813/ /pubmed/35937342 http://dx.doi.org/10.3389/fpls.2022.937767 Text en Copyright © 2022 Feng, Li, Xu, Gao, Wu, Wu, Zhao, Wang, Wang, Li, Hu, Chen, Deng, Li, Chen and Zuo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Feng, Zhiming Li, Mingyou Xu, Zhiwen Gao, Peng Wu, Yunyu Wu, Keting Zhao, Jianhua Wang, Xiaoqiu Wang, Jianan Li, Mengchen Hu, Keming Chen, Hongqi Deng, Yiwen Li, Aihong Chen, Zongxiang Zuo, Shimin Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene |
title | Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene |
title_full | Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene |
title_fullStr | Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene |
title_full_unstemmed | Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene |
title_short | Development of Rice Variety With Durable and Broad-Spectrum Resistance to Blast Disease Through Marker-Assisted Introduction of Pigm Gene |
title_sort | development of rice variety with durable and broad-spectrum resistance to blast disease through marker-assisted introduction of pigm gene |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354813/ https://www.ncbi.nlm.nih.gov/pubmed/35937342 http://dx.doi.org/10.3389/fpls.2022.937767 |
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