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A Structured Scaffold Featuring Biomimetic Heterogeneous Architecture for the Regeneration of Critical-Size Bone Defects

The regeneration of critical-size bone defects on long bones has remained a significant challenge because of the complex anatomical structure and vascular network. In such circumstances, current biomaterial forms with homogeneous structure and function can hardly satisfy the need for both osteogenes...

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Autores principales: Wang, Lingjun, Mao, Jiannan, Cai, Feng, Tang, Jincheng, Xi, Kun, Feng, Yu, Xu, Yichang, Liang, Xiao, Gu, Yong, Chen, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354842/
https://www.ncbi.nlm.nih.gov/pubmed/35935476
http://dx.doi.org/10.3389/fbioe.2022.927050
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author Wang, Lingjun
Mao, Jiannan
Cai, Feng
Tang, Jincheng
Xi, Kun
Feng, Yu
Xu, Yichang
Liang, Xiao
Gu, Yong
Chen, Liang
author_facet Wang, Lingjun
Mao, Jiannan
Cai, Feng
Tang, Jincheng
Xi, Kun
Feng, Yu
Xu, Yichang
Liang, Xiao
Gu, Yong
Chen, Liang
author_sort Wang, Lingjun
collection PubMed
description The regeneration of critical-size bone defects on long bones has remained a significant challenge because of the complex anatomical structure and vascular network. In such circumstances, current biomaterial forms with homogeneous structure and function can hardly satisfy the need for both osteogenesis and angiogenesis. In the current study, a heterogeneous biomimetic structured scaffold was constructed with the help of a 3D printed mold to simultaneously mimic the outer/inner periosteum and intermediate bone matrix of a natural long bone. Because of the reinforcement via modified mesoporous bioactive glass nanoparticles (MBGNs), enhanced structural stability and adequate osteogenic capacity could be achieved for the intermediate layer of this scaffold. Conversely, GelMA incorporated with VEGF-loaded liposome exhibiting controlled release of the angiogenic factor was applied to the inner and outer layers of the scaffold. The resulting heterogeneous structured scaffold was shown to successfully guide bone regeneration and restoration of the natural bone anatomic structure, rendering it a promising candidate for future orthopedic clinical studies.
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spelling pubmed-93548422022-08-06 A Structured Scaffold Featuring Biomimetic Heterogeneous Architecture for the Regeneration of Critical-Size Bone Defects Wang, Lingjun Mao, Jiannan Cai, Feng Tang, Jincheng Xi, Kun Feng, Yu Xu, Yichang Liang, Xiao Gu, Yong Chen, Liang Front Bioeng Biotechnol Bioengineering and Biotechnology The regeneration of critical-size bone defects on long bones has remained a significant challenge because of the complex anatomical structure and vascular network. In such circumstances, current biomaterial forms with homogeneous structure and function can hardly satisfy the need for both osteogenesis and angiogenesis. In the current study, a heterogeneous biomimetic structured scaffold was constructed with the help of a 3D printed mold to simultaneously mimic the outer/inner periosteum and intermediate bone matrix of a natural long bone. Because of the reinforcement via modified mesoporous bioactive glass nanoparticles (MBGNs), enhanced structural stability and adequate osteogenic capacity could be achieved for the intermediate layer of this scaffold. Conversely, GelMA incorporated with VEGF-loaded liposome exhibiting controlled release of the angiogenic factor was applied to the inner and outer layers of the scaffold. The resulting heterogeneous structured scaffold was shown to successfully guide bone regeneration and restoration of the natural bone anatomic structure, rendering it a promising candidate for future orthopedic clinical studies. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9354842/ /pubmed/35935476 http://dx.doi.org/10.3389/fbioe.2022.927050 Text en Copyright © 2022 Wang, Mao, Cai, Tang, Xi, Feng, Xu, Liang, Gu and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wang, Lingjun
Mao, Jiannan
Cai, Feng
Tang, Jincheng
Xi, Kun
Feng, Yu
Xu, Yichang
Liang, Xiao
Gu, Yong
Chen, Liang
A Structured Scaffold Featuring Biomimetic Heterogeneous Architecture for the Regeneration of Critical-Size Bone Defects
title A Structured Scaffold Featuring Biomimetic Heterogeneous Architecture for the Regeneration of Critical-Size Bone Defects
title_full A Structured Scaffold Featuring Biomimetic Heterogeneous Architecture for the Regeneration of Critical-Size Bone Defects
title_fullStr A Structured Scaffold Featuring Biomimetic Heterogeneous Architecture for the Regeneration of Critical-Size Bone Defects
title_full_unstemmed A Structured Scaffold Featuring Biomimetic Heterogeneous Architecture for the Regeneration of Critical-Size Bone Defects
title_short A Structured Scaffold Featuring Biomimetic Heterogeneous Architecture for the Regeneration of Critical-Size Bone Defects
title_sort structured scaffold featuring biomimetic heterogeneous architecture for the regeneration of critical-size bone defects
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354842/
https://www.ncbi.nlm.nih.gov/pubmed/35935476
http://dx.doi.org/10.3389/fbioe.2022.927050
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