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Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries)
In seasonal estrus, it is well known that melatonin-regulated biorhythm plays a key role. Some studies indicate that the adrenal gland plays an important role in reproduction in mammals, but the molecular mechanism is not clear. This study used an artificially controlled light photoperiod model, com...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354845/ https://www.ncbi.nlm.nih.gov/pubmed/35937294 http://dx.doi.org/10.3389/fvets.2022.888207 |
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author | Du, Xiaolong He, Xiaoyun Liu, Qingqing Liu, Qiuyue Di, Ran Chu, Mingxing |
author_facet | Du, Xiaolong He, Xiaoyun Liu, Qingqing Liu, Qiuyue Di, Ran Chu, Mingxing |
author_sort | Du, Xiaolong |
collection | PubMed |
description | In seasonal estrus, it is well known that melatonin-regulated biorhythm plays a key role. Some studies indicate that the adrenal gland plays an important role in reproduction in mammals, but the molecular mechanism is not clear. This study used an artificially controlled light photoperiod model, combined with RNA-seq technology and bioinformatics analysis, to analyze the messenger RNA (mRNA) and microRNA (miRNA) of ewe (Sunite) adrenal glands under different photoperiod treatments. After identification, the key candidate genes GRHL2, CENPF, FGF16 and SLC25A30 that photoperiod affects reproduction were confirmed. The miRNAs (oar-miR-544-3p, oar-miR-411b-5p, oar-miR-376e-3p, oar-miR-376d, oar-miR-376b-3p, oar-miR-376a-3p) were specifically expressed in the adrenal gland. The candidate mRNA-miRNA pairs (e.g., SLC25A30 coagulated by novel miRNA554, novel miRNA555 and novel miRNA559) may affect seasonal estrus. In summary, we constructed relation network of the mRNAs and miRNAs of sheep adrenal glands using RNA sequencing and bioinformatics analysis, thereby, providing a valuable genetic variation resource for sheep genome research, which will contribute to the study of complex traits in sheep. |
format | Online Article Text |
id | pubmed-9354845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93548452022-08-06 Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries) Du, Xiaolong He, Xiaoyun Liu, Qingqing Liu, Qiuyue Di, Ran Chu, Mingxing Front Vet Sci Veterinary Science In seasonal estrus, it is well known that melatonin-regulated biorhythm plays a key role. Some studies indicate that the adrenal gland plays an important role in reproduction in mammals, but the molecular mechanism is not clear. This study used an artificially controlled light photoperiod model, combined with RNA-seq technology and bioinformatics analysis, to analyze the messenger RNA (mRNA) and microRNA (miRNA) of ewe (Sunite) adrenal glands under different photoperiod treatments. After identification, the key candidate genes GRHL2, CENPF, FGF16 and SLC25A30 that photoperiod affects reproduction were confirmed. The miRNAs (oar-miR-544-3p, oar-miR-411b-5p, oar-miR-376e-3p, oar-miR-376d, oar-miR-376b-3p, oar-miR-376a-3p) were specifically expressed in the adrenal gland. The candidate mRNA-miRNA pairs (e.g., SLC25A30 coagulated by novel miRNA554, novel miRNA555 and novel miRNA559) may affect seasonal estrus. In summary, we constructed relation network of the mRNAs and miRNAs of sheep adrenal glands using RNA sequencing and bioinformatics analysis, thereby, providing a valuable genetic variation resource for sheep genome research, which will contribute to the study of complex traits in sheep. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9354845/ /pubmed/35937294 http://dx.doi.org/10.3389/fvets.2022.888207 Text en Copyright © 2022 Du, He, Liu, Liu, Di and Chu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Du, Xiaolong He, Xiaoyun Liu, Qingqing Liu, Qiuyue Di, Ran Chu, Mingxing Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries) |
title | Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries) |
title_full | Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries) |
title_fullStr | Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries) |
title_full_unstemmed | Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries) |
title_short | Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries) |
title_sort | identification of photoperiod-induced specific mirnas in the adrenal glands of sunite sheep (ovis aries) |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354845/ https://www.ncbi.nlm.nih.gov/pubmed/35937294 http://dx.doi.org/10.3389/fvets.2022.888207 |
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