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Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea

Gray mold caused by Botrytis cinerea is detrimental to plants and fruits. Endophytes have been shown to modify plant disease severity in functional assays. We conducted this study to investigate the endophytic strain Bacillus K1 with excellently antagonistic B. cinerea from the wild grape endosphere...

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Autores principales: Li, Peiqian, Feng, Baozhen, Yao, Zhen, Wei, Bohui, Zhao, Yanfei, Shi, Shouguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355035/
https://www.ncbi.nlm.nih.gov/pubmed/35935203
http://dx.doi.org/10.3389/fmicb.2022.935675
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author Li, Peiqian
Feng, Baozhen
Yao, Zhen
Wei, Bohui
Zhao, Yanfei
Shi, Shouguo
author_facet Li, Peiqian
Feng, Baozhen
Yao, Zhen
Wei, Bohui
Zhao, Yanfei
Shi, Shouguo
author_sort Li, Peiqian
collection PubMed
description Gray mold caused by Botrytis cinerea is detrimental to plants and fruits. Endophytes have been shown to modify plant disease severity in functional assays. We conducted this study to investigate the endophytic strain Bacillus K1 with excellently antagonistic B. cinerea from the wild grape endosphere. We identified a wild grape endophytic strain K1 with high antifungal activity against B. cinerea both in vitro and in vivo. Combining the phylogenetic results based on 16S rDNA and genome sequencing, K1 was assigned as Bacillus subtilis. The in vitro results displayed that K1 and its volatile substances could significantly inhibit the mycelia growth of B. cinerea. Grape fruit inoculated with Bacillus K1 showed lower gray mold during treatment. The higher levels of defense-related enzymes, including peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase, were induced in grapes after inoculation. Scanning electron microscopy (SEM) suggested that K1 inhibited mycelial growth via bacterial colonization and antibiosis in grapes. The gas chromatography–mass spectrometry analysis identified 33 volatiles in which dibutyl phthalate was the major compound accounting for 74.28%. Dibutyl phthalate demonstrated strong activity in suppressing the mycelia growth of B. cinerea. Genome bioinformatics analysis revealed that the K1 chromosome harbored many known biosynthesis gene clusters encoding subtilosin, bacillaene, bacillibactin, bacilysin, and fengycin. This study provides a potential biological agent to control diseases of post-harvest grape fruit and improves our understanding of the possible biocontrol mechanisms of the Bacillus strain.
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spelling pubmed-93550352022-08-06 Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea Li, Peiqian Feng, Baozhen Yao, Zhen Wei, Bohui Zhao, Yanfei Shi, Shouguo Front Microbiol Microbiology Gray mold caused by Botrytis cinerea is detrimental to plants and fruits. Endophytes have been shown to modify plant disease severity in functional assays. We conducted this study to investigate the endophytic strain Bacillus K1 with excellently antagonistic B. cinerea from the wild grape endosphere. We identified a wild grape endophytic strain K1 with high antifungal activity against B. cinerea both in vitro and in vivo. Combining the phylogenetic results based on 16S rDNA and genome sequencing, K1 was assigned as Bacillus subtilis. The in vitro results displayed that K1 and its volatile substances could significantly inhibit the mycelia growth of B. cinerea. Grape fruit inoculated with Bacillus K1 showed lower gray mold during treatment. The higher levels of defense-related enzymes, including peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase, were induced in grapes after inoculation. Scanning electron microscopy (SEM) suggested that K1 inhibited mycelial growth via bacterial colonization and antibiosis in grapes. The gas chromatography–mass spectrometry analysis identified 33 volatiles in which dibutyl phthalate was the major compound accounting for 74.28%. Dibutyl phthalate demonstrated strong activity in suppressing the mycelia growth of B. cinerea. Genome bioinformatics analysis revealed that the K1 chromosome harbored many known biosynthesis gene clusters encoding subtilosin, bacillaene, bacillibactin, bacilysin, and fengycin. This study provides a potential biological agent to control diseases of post-harvest grape fruit and improves our understanding of the possible biocontrol mechanisms of the Bacillus strain. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9355035/ /pubmed/35935203 http://dx.doi.org/10.3389/fmicb.2022.935675 Text en Copyright © 2022 Li, Feng, Yao, Wei, Zhao and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Peiqian
Feng, Baozhen
Yao, Zhen
Wei, Bohui
Zhao, Yanfei
Shi, Shouguo
Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea
title Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea
title_full Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea
title_fullStr Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea
title_full_unstemmed Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea
title_short Antifungal Activity of Endophytic Bacillus K1 Against Botrytis cinerea
title_sort antifungal activity of endophytic bacillus k1 against botrytis cinerea
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355035/
https://www.ncbi.nlm.nih.gov/pubmed/35935203
http://dx.doi.org/10.3389/fmicb.2022.935675
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