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The membrane-actin linker ezrin acts as a sliding anchor

Protein linkages to filamentous (F)–actin provide the cell membrane with mechanical stability and support intricate membrane architectures. However, the actin cytoskeleton is highly dynamic and undergoes rapid changes in shape during cell motility and other processes. The molecular mechanisms that g...

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Detalles Bibliográficos
Autores principales: Korkmazhan, Elgin, Dunn, Alexander R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355349/
https://www.ncbi.nlm.nih.gov/pubmed/35930643
http://dx.doi.org/10.1126/sciadv.abo2779
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author Korkmazhan, Elgin
Dunn, Alexander R.
author_facet Korkmazhan, Elgin
Dunn, Alexander R.
author_sort Korkmazhan, Elgin
collection PubMed
description Protein linkages to filamentous (F)–actin provide the cell membrane with mechanical stability and support intricate membrane architectures. However, the actin cytoskeleton is highly dynamic and undergoes rapid changes in shape during cell motility and other processes. The molecular mechanisms that generate a mechanically robust yet fluid connection between the membrane and actin cytoskeleton remain poorly understood. Here, we adapted a single-molecule optical trap assay to examine how the prototypical membrane-actin linker ezrin acts to anchor F-actin to the cell membrane. We find that ezrin forms a complex that slides along F-actin over micrometer distances while resisting detachment by forces oriented perpendicular to the filament axis. The ubiquity of ezrin and analogous proteins suggests that sliding anchors such as ezrin may constitute an important but overlooked element in the construction of the actin cytoskeleton.
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spelling pubmed-93553492022-08-18 The membrane-actin linker ezrin acts as a sliding anchor Korkmazhan, Elgin Dunn, Alexander R. Sci Adv Biomedicine and Life Sciences Protein linkages to filamentous (F)–actin provide the cell membrane with mechanical stability and support intricate membrane architectures. However, the actin cytoskeleton is highly dynamic and undergoes rapid changes in shape during cell motility and other processes. The molecular mechanisms that generate a mechanically robust yet fluid connection between the membrane and actin cytoskeleton remain poorly understood. Here, we adapted a single-molecule optical trap assay to examine how the prototypical membrane-actin linker ezrin acts to anchor F-actin to the cell membrane. We find that ezrin forms a complex that slides along F-actin over micrometer distances while resisting detachment by forces oriented perpendicular to the filament axis. The ubiquity of ezrin and analogous proteins suggests that sliding anchors such as ezrin may constitute an important but overlooked element in the construction of the actin cytoskeleton. American Association for the Advancement of Science 2022-08-05 /pmc/articles/PMC9355349/ /pubmed/35930643 http://dx.doi.org/10.1126/sciadv.abo2779 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Korkmazhan, Elgin
Dunn, Alexander R.
The membrane-actin linker ezrin acts as a sliding anchor
title The membrane-actin linker ezrin acts as a sliding anchor
title_full The membrane-actin linker ezrin acts as a sliding anchor
title_fullStr The membrane-actin linker ezrin acts as a sliding anchor
title_full_unstemmed The membrane-actin linker ezrin acts as a sliding anchor
title_short The membrane-actin linker ezrin acts as a sliding anchor
title_sort membrane-actin linker ezrin acts as a sliding anchor
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355349/
https://www.ncbi.nlm.nih.gov/pubmed/35930643
http://dx.doi.org/10.1126/sciadv.abo2779
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