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The membrane-actin linker ezrin acts as a sliding anchor
Protein linkages to filamentous (F)–actin provide the cell membrane with mechanical stability and support intricate membrane architectures. However, the actin cytoskeleton is highly dynamic and undergoes rapid changes in shape during cell motility and other processes. The molecular mechanisms that g...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355349/ https://www.ncbi.nlm.nih.gov/pubmed/35930643 http://dx.doi.org/10.1126/sciadv.abo2779 |
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author | Korkmazhan, Elgin Dunn, Alexander R. |
author_facet | Korkmazhan, Elgin Dunn, Alexander R. |
author_sort | Korkmazhan, Elgin |
collection | PubMed |
description | Protein linkages to filamentous (F)–actin provide the cell membrane with mechanical stability and support intricate membrane architectures. However, the actin cytoskeleton is highly dynamic and undergoes rapid changes in shape during cell motility and other processes. The molecular mechanisms that generate a mechanically robust yet fluid connection between the membrane and actin cytoskeleton remain poorly understood. Here, we adapted a single-molecule optical trap assay to examine how the prototypical membrane-actin linker ezrin acts to anchor F-actin to the cell membrane. We find that ezrin forms a complex that slides along F-actin over micrometer distances while resisting detachment by forces oriented perpendicular to the filament axis. The ubiquity of ezrin and analogous proteins suggests that sliding anchors such as ezrin may constitute an important but overlooked element in the construction of the actin cytoskeleton. |
format | Online Article Text |
id | pubmed-9355349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-93553492022-08-18 The membrane-actin linker ezrin acts as a sliding anchor Korkmazhan, Elgin Dunn, Alexander R. Sci Adv Biomedicine and Life Sciences Protein linkages to filamentous (F)–actin provide the cell membrane with mechanical stability and support intricate membrane architectures. However, the actin cytoskeleton is highly dynamic and undergoes rapid changes in shape during cell motility and other processes. The molecular mechanisms that generate a mechanically robust yet fluid connection between the membrane and actin cytoskeleton remain poorly understood. Here, we adapted a single-molecule optical trap assay to examine how the prototypical membrane-actin linker ezrin acts to anchor F-actin to the cell membrane. We find that ezrin forms a complex that slides along F-actin over micrometer distances while resisting detachment by forces oriented perpendicular to the filament axis. The ubiquity of ezrin and analogous proteins suggests that sliding anchors such as ezrin may constitute an important but overlooked element in the construction of the actin cytoskeleton. American Association for the Advancement of Science 2022-08-05 /pmc/articles/PMC9355349/ /pubmed/35930643 http://dx.doi.org/10.1126/sciadv.abo2779 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Korkmazhan, Elgin Dunn, Alexander R. The membrane-actin linker ezrin acts as a sliding anchor |
title | The membrane-actin linker ezrin acts as a sliding anchor |
title_full | The membrane-actin linker ezrin acts as a sliding anchor |
title_fullStr | The membrane-actin linker ezrin acts as a sliding anchor |
title_full_unstemmed | The membrane-actin linker ezrin acts as a sliding anchor |
title_short | The membrane-actin linker ezrin acts as a sliding anchor |
title_sort | membrane-actin linker ezrin acts as a sliding anchor |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355349/ https://www.ncbi.nlm.nih.gov/pubmed/35930643 http://dx.doi.org/10.1126/sciadv.abo2779 |
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