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Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode

Ditchless rice-crayfish co-culture is an emerging model of rice-crayfish farming that circumvents the potential hazards of digging ditches in traditional rice-crayfish farming. However, due to the complex interactions among crayfish, ambient microbiota, and environmental variables, it is necessary t...

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Autores principales: Huang, Xiaoli, Li, Minghao, Huang, Ya, Yang, Hai, Geng, Yi, Ouyang, Ping, Chen, Defang, Yin, Lizi, Yang, Shiyong, Jiang, Jun, Luo, Wei, He, Zhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355531/
https://www.ncbi.nlm.nih.gov/pubmed/35935240
http://dx.doi.org/10.3389/fmicb.2022.892026
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author Huang, Xiaoli
Li, Minghao
Huang, Ya
Yang, Hai
Geng, Yi
Ouyang, Ping
Chen, Defang
Yin, Lizi
Yang, Shiyong
Jiang, Jun
Luo, Wei
He, Zhi
author_facet Huang, Xiaoli
Li, Minghao
Huang, Ya
Yang, Hai
Geng, Yi
Ouyang, Ping
Chen, Defang
Yin, Lizi
Yang, Shiyong
Jiang, Jun
Luo, Wei
He, Zhi
author_sort Huang, Xiaoli
collection PubMed
description Ditchless rice-crayfish co-culture is an emerging model of rice-crayfish farming that circumvents the potential hazards of digging ditches in traditional rice-crayfish farming. However, due to the complex interactions among crayfish, ambient microbiota, and environmental variables, it is necessary to assess the differences in bacterial structure between ditchless and traditional rice-crayfish culture. In this study, the crayfish culture area in the Sichuan basin was selected as the study area, and the bacterial communities of two rice-crayfish culture systems were compared by high-throughput sequencing of 16S rDNA. The results showed that the ditchless system had lower water depth, higher dissolved oxygen, lower total ammonia nitrogen and lower morbidity. There are intuitive differences in the composition of environmental bacterial communities due to environmental changes, even if they are similar in composition at the phylum level. Microbiota in sediments from ditchless systems appear to produce less ammonia nitrogen. The abundance of the pathogens colonizing the intestine of ditchless crayfish was lower than ditched one, and the composition was similar to water. Ditch-farmed crayfish appear to be more susceptible to environmental microbes and have a more fragile intestinal structure. Water depth and dissolved oxygen are the main environmental factors that determine the distribution of microbiota. This study is the first to investigate the bacterial ecology of a ditchless rice- crayfish farming system. The results show that the ditchless rice-crayfish culture model has a more superior bacterial system than the traditional rice-crayfish culture.
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spelling pubmed-93555312022-08-06 Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode Huang, Xiaoli Li, Minghao Huang, Ya Yang, Hai Geng, Yi Ouyang, Ping Chen, Defang Yin, Lizi Yang, Shiyong Jiang, Jun Luo, Wei He, Zhi Front Microbiol Microbiology Ditchless rice-crayfish co-culture is an emerging model of rice-crayfish farming that circumvents the potential hazards of digging ditches in traditional rice-crayfish farming. However, due to the complex interactions among crayfish, ambient microbiota, and environmental variables, it is necessary to assess the differences in bacterial structure between ditchless and traditional rice-crayfish culture. In this study, the crayfish culture area in the Sichuan basin was selected as the study area, and the bacterial communities of two rice-crayfish culture systems were compared by high-throughput sequencing of 16S rDNA. The results showed that the ditchless system had lower water depth, higher dissolved oxygen, lower total ammonia nitrogen and lower morbidity. There are intuitive differences in the composition of environmental bacterial communities due to environmental changes, even if they are similar in composition at the phylum level. Microbiota in sediments from ditchless systems appear to produce less ammonia nitrogen. The abundance of the pathogens colonizing the intestine of ditchless crayfish was lower than ditched one, and the composition was similar to water. Ditch-farmed crayfish appear to be more susceptible to environmental microbes and have a more fragile intestinal structure. Water depth and dissolved oxygen are the main environmental factors that determine the distribution of microbiota. This study is the first to investigate the bacterial ecology of a ditchless rice- crayfish farming system. The results show that the ditchless rice-crayfish culture model has a more superior bacterial system than the traditional rice-crayfish culture. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9355531/ /pubmed/35935240 http://dx.doi.org/10.3389/fmicb.2022.892026 Text en Copyright © 2022 Huang, Li, Huang, Yang, Geng, Ouyang, Chen, Yin, Yang, Jiang, Luo and He. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Huang, Xiaoli
Li, Minghao
Huang, Ya
Yang, Hai
Geng, Yi
Ouyang, Ping
Chen, Defang
Yin, Lizi
Yang, Shiyong
Jiang, Jun
Luo, Wei
He, Zhi
Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode
title Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode
title_full Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode
title_fullStr Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode
title_full_unstemmed Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode
title_short Microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode
title_sort microbiome analysis reveals microecological advantages of emerging ditchless rice-crayfish co-culture mode
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355531/
https://www.ncbi.nlm.nih.gov/pubmed/35935240
http://dx.doi.org/10.3389/fmicb.2022.892026
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