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Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil

Rhizosphere microorganisms are essential parts in maintaining soil ecological functions. Reforestation using genetically modified trees might have great potential to enhance tree production in biotic and abiotic stress, however, their long-term impact on rhizosphere microorganisms is scant. In this...

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Autores principales: Zheng, Yu, Lv, Guan Bin, Chen, Kun, Yu, Qibin, Niu, Ben, Jiang, Jing, Liu, Guifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355801/
https://www.ncbi.nlm.nih.gov/pubmed/35942008
http://dx.doi.org/10.3389/fbioe.2022.965209
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author Zheng, Yu
Lv, Guan Bin
Chen, Kun
Yu, Qibin
Niu, Ben
Jiang, Jing
Liu, Guifeng
author_facet Zheng, Yu
Lv, Guan Bin
Chen, Kun
Yu, Qibin
Niu, Ben
Jiang, Jing
Liu, Guifeng
author_sort Zheng, Yu
collection PubMed
description Rhizosphere microorganisms are essential parts in maintaining soil ecological functions. Reforestation using genetically modified trees might have great potential to enhance tree production in biotic and abiotic stress, however, their long-term impact on rhizosphere microorganisms is scant. In this study, we studied soil enzyme activities and composition of rhizosphere microorganisms in 2-year-old transgenic PaGLK overexpression (OE), repressed expression (RE) and wild-type (WT) poplar (P. alba × P.berlinensis). The root exudates of PaGLK transgenic poplar (P.alba × P. berlinensis) were analyzed by liquid chromatography-mass spectrometry (LC-MS). The results showed that there were significant difference for soil sucrase, urease, catalase, neutral protease and cellulase between the transgenic and WT lines at different growth periods. Alpha diversity analysis showed that bacterial community abundance and diversity for RE lines were significantly lower than WT (p < 0.05), while RE lines for fungi were significantly higher than WT lines. At the genus level, Burkholderia was the dominant group of rhizosphere bacterial community, and the relative abundance for RE was significantly higher than WT. Tomentella was the dominant group for fungi community. Serendipita for RE was significantly higher than WT and OE. Main metabolite contents of (S)-ACPA, geniposidic acid, agnuside, hydroquinone and pyranocoumarins were significantly different among transgenic lines. These results suggest that transgenic activities have effects on root exudates, rhizosphere soil enzyme activities and soil microbial community composition, but long term effects need to be further investigated.
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spelling pubmed-93558012022-08-07 Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil Zheng, Yu Lv, Guan Bin Chen, Kun Yu, Qibin Niu, Ben Jiang, Jing Liu, Guifeng Front Bioeng Biotechnol Bioengineering and Biotechnology Rhizosphere microorganisms are essential parts in maintaining soil ecological functions. Reforestation using genetically modified trees might have great potential to enhance tree production in biotic and abiotic stress, however, their long-term impact on rhizosphere microorganisms is scant. In this study, we studied soil enzyme activities and composition of rhizosphere microorganisms in 2-year-old transgenic PaGLK overexpression (OE), repressed expression (RE) and wild-type (WT) poplar (P. alba × P.berlinensis). The root exudates of PaGLK transgenic poplar (P.alba × P. berlinensis) were analyzed by liquid chromatography-mass spectrometry (LC-MS). The results showed that there were significant difference for soil sucrase, urease, catalase, neutral protease and cellulase between the transgenic and WT lines at different growth periods. Alpha diversity analysis showed that bacterial community abundance and diversity for RE lines were significantly lower than WT (p < 0.05), while RE lines for fungi were significantly higher than WT lines. At the genus level, Burkholderia was the dominant group of rhizosphere bacterial community, and the relative abundance for RE was significantly higher than WT. Tomentella was the dominant group for fungi community. Serendipita for RE was significantly higher than WT and OE. Main metabolite contents of (S)-ACPA, geniposidic acid, agnuside, hydroquinone and pyranocoumarins were significantly different among transgenic lines. These results suggest that transgenic activities have effects on root exudates, rhizosphere soil enzyme activities and soil microbial community composition, but long term effects need to be further investigated. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9355801/ /pubmed/35942008 http://dx.doi.org/10.3389/fbioe.2022.965209 Text en Copyright © 2022 Zheng, Lv, Chen, Yu, Niu, Jiang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zheng, Yu
Lv, Guan Bin
Chen, Kun
Yu, Qibin
Niu, Ben
Jiang, Jing
Liu, Guifeng
Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil
title Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil
title_full Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil
title_fullStr Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil
title_full_unstemmed Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil
title_short Impact of PaGLK transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil
title_sort impact of paglk transgenic poplar on microbial community and soil enzyme activity in rhizosphere soil
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355801/
https://www.ncbi.nlm.nih.gov/pubmed/35942008
http://dx.doi.org/10.3389/fbioe.2022.965209
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